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2-3
Fine sectioning of specimens
In general, both single sections as well as
ribbons can be generated with the STS.
Note:
Ribbons can only generated when the
correct clearance angle is selected and
the upper and lower edge of the
paraffin block is parallel and even.
Ribbons are generated when the upper edge
of the last section "clings" to the blade. The
lower edge of the following sections is then
being pressed on the upper edge of the
previous section. This way, the sections stick
together.
When cutting fine sections, the ribbons or
single sections are guided directly into the
water bath via the closed transfer bridge
(fig. 8.7):
For this, pull back the lever of the transfer
bridge (fig. 8.7) until the bridge lies on the rim
of the water bath.
Activate the lever (fig. 8.8) of the detaching
tongue to loosen the sections and/or the
ribbon.
Section and/or ribbon now glide on the water
flow into the water bath.
Then pull back the transfer bridge to
minimize the mixture of cold and heated
water.
Note:
16
Thermo Scientific Section Transfer System Microm STS
The STS offers two different possibilities to
evaluate the quality of the sections produced
and to make a selection:
First quality evaluation of sections:
Only after having evaluated the produced fine
sections on the transfer surface for good
quality, activate the detaching tongue to guide
the sections via the closed transfer bridge into
the water bath.
Second quality evaluation of sections:
The stretched sections are illuminated in the
water bath by the section illumination (fig. 5.7)
from below. This way, evaluation is even
better. Sections with the best quality can be
picked up by a slide.
Microm International GmbH
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English
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