Read A Plate - Molecular Devices SpectraMax M2 User Manual

Fluorescence Fluorophores Wavelengths
Fluorophore
HPPA
4-MeU, NADH, NADPH
Biotinidinase
PKU
Green Fluorescent
Protein
Attophos /Attofluor
FITC
Ethidium Homodimer
(DNA)
TRITC, Ethidium Bromide
Texas Red
TAMRA
Tryptophan

Read a Plate

CAUTION! Before you read standard 96-well or 384-well plates from the top, you must
insert the plate adapter into the plate drawer. Before you do a bottom read, or when
you use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the
plate adapter from the plate drawer. See
Before you place a plate in the drawer, the underside of the plate must be dry. If the plate
has fluid on the underside, you must dry it with a lint-free cloth before you place it in the
drawer.
1. On the rear of the instrument, turn on the power switch.
The instrument carries out a diagnostic check. During this check, the plate drawer opens,
and then closes. When the diagnostic check completes, the plate drawer opens and
remains open.
2. Power on the computer.
3. Start the SoftMax Pro Software and connect the software to the instrument.
4. In the software, in the Ribbon, on the Home tab, click
the Settings dialog where you define the acquisition settings for the Plate section.
0112-0102 E
Excitation Wavelength (nm)
320
355
355
390
390
444
485
530
544
584
547
280
Plate Adapter on page
Chapter 4: Operating the Instrument
Emission Wavelength (nm)
405
460
544
485
510
555
538
620
590
612
580
340
19.
Acquisition Settings to display
22