Molecular Devices SpectraMax M2 User Manual page 16

Temp
The Temp p and q buttons enable you to enter the set point at which to regulate the
temperature inside the cuvette chamber and the plate chamber. The temperature for the
instrument chambers is isothermal at ambient temperature as well as at elevated
temperatures. The cuvette chamber temperature displays on the instrument control panel
and the plate chamber temperature displays in the software.
You cannot set a temperature beyond the upper (45°C) or lower (4°C above ambient)
instrument limits. To set the temperature at 45°C, the ambient temperature of the room
must be at least 20°C.
Wavelength (λ)
The λ p and q buttons enable you to set the wavelengths to read the cuvette. The control
panel contains two sets of λ wavelength buttons. The left buttons enable you to set the
absorbance/excitation (fluorescence) wavelength and the right buttons enable you to set the
emission (fluorescence) wavelength.
Note: The control panel does not display the wavelength if you use the software to
set the wavelength.
%T/A/RFU
The %T/A/RFU button enables you to change the display of the cuvette reference read data
between percent transmittance (%T), absorbance (A) or relative fluorescence units (RFU).
Read Cuvette
The Read Cuvette button enables you to start a cuvette read.
Note: The Read Cuvette button is disabled when the software does a read.
REF
The REF button enables you to do a cuvette reference read of buffer, water, or air. The
software uses this value as I
reference read, the instrument uses the I
Note: The REF button is disabled when the software does a read.
Drawer
The Drawer button enables you to open and close the plate drawer.
0112-0102 E
to calculate Absorbance or % Transmittance. If you do not do a
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value that is stored in the instrument.
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Chapter 3: Getting Started
16