Molecular Devices SpectraMax M2 User Manual page 47

Table A-2: Fluorescence Intensity Performance
Item
Sensitivity
Sensitivity (cuvette)
Excitation wavelength range
Emission wavelength range
Wavelength selection
Bandwidth (excitation, emission) 9 nm, 9 nm
Number of excitation/emission
pairs per plate
Dynamic range
System validation
Light source
Average lamp lifetime
Detector
0112-0102 E
Description
Top Read (M2 and M2e)
3.0 fmol/well in 200 µL FITC (96 well microplate and 15 pM)
3.0 fmol/well in 75 µL FITC (384 well microplate and 40 pM)
Bottom Read (M2e only)
5.0 fmol/well in 200 µL FITC (96 well microplate and 25 pM)
5.0 fmol/well in 75 µL FITC (384 well microplate and 67 pM)
< 15 pM fluorescein
250-850 nm
360-850 nm (M2)
250-850 nm (M2e)
Monochromators, tunable in 1 nm increments
4
6
10 in 96-well black plates: auto gain circuitry
Self-calibrating with built-in fluorescence calibrators
Xenon flash lamp (1 joule/flash)
1 billion flashes
Photomultiplier (R3896)
Appendix A: Specifications
47