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A14.1065
Inverted Biological Phase Contrast Microscope
A16.1065
Inverted LED Fluorescent Microscope, B,G,U
Instruction Manual
This instruction manual is intended for users of the Inverted Series Biological Microscope
A16.1065. To ensure safety use, perform the best performance and to familiarize yourself with
the use of this microscope, please read this manual carefully before operating this product.
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Table of Contents
Related Manuals for OPTO-EDU A14.1065
Summary of Contents for OPTO-EDU A14.1065
- Page 1 A14.1065 Inverted Biological Phase Contrast Microscope A16.1065 Inverted LED Fluorescent Microscope, B,G,U Instruction Manual This instruction manual is intended for users of the Inverted Series Biological Microscope A16.1065. To ensure safety use, perform the best performance and to familiarize yourself with...
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Page 2: Table Of Contents
Contents Before Using …………………………………………………………………… Safety Symbol………………………………………………………………… 1 Safety Precautions …………………………………………………………… Care and Maintenance………………………………………………………… 2 Chapter 1 Introduction ………………………………………………………… 4 1.1 Technical Specifications ………………………………………………… 1.1.1 Main Technical Specifications and Configuration…………………… 4 1.1.2 Objective Parameters………………………………………………… 5 1.1.3 Electrical Parameters………………………………………………… 5 1.2 Components ……………………………………………………………… 6 Chapter 2 Overview of Agencies ………………………………………………... - Page 3 3.10 Operating the LCD …………………………………………………… 3.10.1 The status of LCD ………………………………………………… 3.10.2 Operating the illumination switch ……………………………… Chapter 4 Assembly……………………………………………………………… 18 …………………………………………………… 18 4.1 Installation diagram ……………………………………………………… 19 4.2 Installation Steps 4.2.1 Installing the Condenser …………………………………………… 19 4.2.2 Installing the Phase Contrast Slider ………………………………… 4.2.3 Installing the Moving Ruler …………………………………………...
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Page 4: Before Using
Before Using A16.1065 Safety Symbol Symbol Description The surface is hot and cannot be touched with bare hands. Please read this manual carefully before using the product. Incorrect usage can cause personal injury or property damage. The main power switch is turned on. The main power switch is turned off. -
Page 5: Care And Maintenance
A16.1065 5. Grounding the machine to avoid lightning strike. To ensure safety, always make sure the main switch is in "O" (disconnecting) and cut off the power while waiting for the LED lights and the lamp room to cool completely. Input voltage check: the input voltage indicated on the back of the microscope is consistent with the supply voltage, otherwise the microscope will be seriously damaged. - Page 6 A16.1065 Because the solvent such as ether and alcohol are extremely flammable, do not operate power switch of various electrical equipment when using, and keep away from open fire, please ensure indoor ventilation. Do not use organic solvents to clean the non-optical parts of the microscope. Please use neutral detergents when cleaning.
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Page 7: Chapter 1 Introduction
Chapter 1 Introduction A16.1065 1.1 Technical Specifications 1.1.1 Main technical specifications and configuration Technical Specifications Device A14.1065 A16.1065 ● ● Extra Wide Field Eyepiece 10× /22 ○ ○ Eyepiece Field Eyepiece 15× /16 ○ ○ Field Eyepiece 20× /12 Seidentopf Viewing Head, alterable angle, ●... -
Page 8: Objective Parameters
Choose component Technical Specifications Infinity plane semi-apochromatic objective（adjustable）20× 、40× 、60× Infinity plane semi-apochromatic contrast objective（adjustable）20× 、40× 、60× Infinity plane semi-apochromatic contrast objective 4× 、10× 、20× 、40× 、60× 1.1.2 Objective parameters Type A14.1065 A16.1065 Magnification 4× 10× 20× 40× 10× 20×... -
Page 9: Components
A16.1065 1.2 Components The microscope A16.1065 components ( following diagrams) . Eyepiece Contrast slider Condenser Viewing head Stage Objective Camera mount Moving stage Nosepiece Mainframe A16.1065... - Page 10 A16.1065 The microscope A16.1065-FL components ( following diagrams). Fluorescent baffle Contrast slider Eyepiece Condenser Upper lighting shading part Viewing head Stage Moving stage Camera mount Fluorescence illumination Objective Nosepiece Mainframe Filter Cube turret A16.1065-FL...
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Page 11: Chapter 2 Overview Of Agencies
Chapter 2 Overview of Agencies A16.1065 A14.1063 Condenser aperture adjustment knob Condenser brightness Condenser rotating knob Fine focus knob Condenser adjustment knob Coarse focus knob aperture diaphragm adjusting knob Power indicator Nosepiece Camera mount Power switch... - Page 12 A16.1065 A16.1063 Bracket horizontal movement knob Bracket vertical movement knob Fluorescence cube turret...
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Page 13: Chapter 3 Regulation And Operation
Chapter 3 Regulation and Operation A16.1065 3.1 Turning the Power on Turn on the power and turn the main switch on the back of the microscope body to "-" (on). Power switch 3.2 Adjusting the Illumination Brightness As shown in the direction of rotation, the light source is enhanced, whereas the light source weakened. -
Page 14: Pallet
A16.1065 3.3.2 Holders There are a variety of holders to choose from. Model Terasaki microplate Universal holder 54mm Petri dish holder/ Slide holder 60 mm Petri dish holder/ Slide holder 90 mm Petri dish holder 3.3.3 Auxiliary stage Connect the auxiliary stage to the left and Auxiliary stage right of the stage to expand the stage width. -
Page 15: Focusing On The Specimens
A16.1065 3.5 Focusing on the Specimens 3.5.1 Focus knob To adjust the focus， rotate the focus knobs on the right and left sides of the microscope to limit screw move the objective up and down. The figure to the right illustrates the relationship between the rotational direction of the focus knobs and the vertical notion of the objective. -
Page 16: Adjusting The Diascopic Illumination Aperture Diaphragm
A16.1065 3.6 Adjusting the Transmitted Illumination Aperture Diaphragm Insert a centering telescope in the eyepiece tube, adjust the focal length, and view the objective pupil plane (a bright circle) and the aperture diaphragm image. Adjust the aperture diaphragm so that the size of the Aperture diaphragm diaphragm image is 70-80% of the size of open/ close lever... -
Page 17: Operation The Phase Contrast Microscope
A16.1065 3.8 Operation the Phase Contrast Microscope Phase contrast microscopy requires phase contrast (PH) objectives and PH sliders. Hollow position Annular diaphragm Centering hold Centering hold (on the side) (on the side) If PH Slider is used, it is possible to center the annular diaphragm. Turn the centering screw of the PH slider to merge the annular diaphragm image with the phase ring image in the objective. -
Page 18: Operating The Episcopic Illumination Microscopy
A16.1065 3.9Operating the Episcopic Illumination Microscopy (for the 410-FL Only) （ 3.9.1 Episcopic illumination A16.1065-FL） Changing the illumination switch to switch between episcopic illumination LED and diascopic illumination fluorescent mode, see section 4.3.2. Fluorescent LED illumination will turn weaker, when rotating direction illumination brightness adjuster as right... -
Page 19: Fluorescence Filter Cube
A16.1065 position, seen from the front of the microscope: If you select the bright-field position, the filter cube is removed from the optical path. The three bright-field positions have the same function. SIGN Rotate the turret so that the fluorescence positions indicated by the arrows on the left and right are the same (all points to “1”... -
Page 20: Operating The Lcd
A16.1065 3.10 Operating the LCD（for A16.1064 series） 3.10.1 The status of LCD Start & working mode Standby SLEEP Lock Time setting TIME 3.10.2 Operating the illumination switch ⚫ Click brightness adjuster：becoming standby mode，it’s“SLEEP”in the screen。 Click again，it becomes working mode。 ⚫... -
Page 21: Chapter 4 Assembly
Chapter 4 Assembly A16.1065 4.1 Installation diagram The order of installation of each component is shown in the following figure, and the digital indicate the installation step. Save the supplied hexagonal spanner. You need it when you change parts. CAUTION Before installation, make sure all the parts are free of dust and dirt. -
Page 22: Installation Steps
4.2 Installation Steps A16.1065 4.2.1 Installing the Condenser Unscrew the captive screws on the condenser a certain distance before aligning the tube with the dovetail groove of the condenser, then pushing lightly to the lowest position horizontally. and tighten the set screws. -
Page 23: Installing The Objective
A16.1065 4.2.4 Installing the Objective 1. Adjust the coarse focus knob until the converter drops to low limit. 2. Screw the objectives with the lowest magnification to the converter from the left or right and then push the nosepiece clockwise to install the other objectives in descending order of magnification. -
Page 24: Assembly Episcopic Illumination Microscopy
4.3 Assembly for Episcopic Illumination Microscopy 4.3.1 Attaching the Filter Cube 1) Remove the cover from the filter cube mounting port. 2) Turn the turret to a position where a Filter cube filter cube can be mounted easily. 3) Check the address on the turret, and align the filter cube with the guide Cover groove before inserting. -
Page 25: Chapter 5 Maintenance And Storage
Chapter 5 Maintenance and Storage A16.1065 5.1 Cleaning Clean or disinfect the lenses and other components according to the following instructions. Cleaning tools ⚫ Blower Soft brush ⚫ ⚫ Soft cotton cloth, lens cleaning tissue, gauze, etc. ⚫ Absolute alcohol (ethyl alcohol or methyl alcohol), medical alcohol ⚫... -
Page 26: Cleaning Off Immersion Oil
A16.1065 Cleaning off Immersion Oil 5.1.3 (1) Wipe off using petroleum benzene. (2) Then, wipe off using absolute alcohol (ethyl alcohol or methyl alcohol) for a better finish. If petroleum benzene is unavailable If petroleum benzene is unavailable, use methyl alcohol. Note that methyl alcohol is less effective, and requires more wipes. -
Page 27: Chapter 6 Troubleshooting
Chapter 6 Troubleshooting A16.1065 6.1 Optical System TROUBLE CAUSE SOLUTION The nosepiece is not in the located Locate the nosepiece properly position (objective and light path not The edge of the field where it clicks coaxial) of view is dark or the brightness is not uniform A lens (the objective, condenser,... -
Page 28: Electrical Parts
A16.1065 6.2 Mechanical System TROUBLE CAUSE SOLUTION image cannot focus Turn it over; Specimen is placed reversely; with the high-power Use standard cover glass with Cover slip is too thick objective thickness of 0.17mm The objective will Turn it over; touch the Specimen Specimen is placed reversely;...