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Related Manuals for Biotage Selekt Enkel
Summary of Contents for Biotage Selekt Enkel
- Page 1 Biotage Selekt Enkel ® User Manual...
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Page 2: Table Of Contents
Search the Results Consumables and Accessories Analysis of the Result Manufacturer Customize the Report Contact Us Add a Report Note Notes Export the Report Base a New Run on a Previous Run Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 3: System Overview
System Overview System Overview QR Reader Biotage Selekt Enkel is an automated flash purification system ® with a built-in QR reader for Biotage Sfär columns, UV detector, ® All Biotage Sfär columns have a QR code that can be read ®... -
Page 4: Solvent Supply
( ) in the About view when the Biotage Solvent Detector option is enabled in the system settings. ® Otherwise, only one solvent detector can be used. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 5: Uv Detector
System Overview The status for each solvent detector connected to the system is displayed in the About view; see Figure 7. Figure 8. The expanded Collection panel. Network Share With the system connected to the network, it is possible to give Figure 7. -
Page 6: Mail Functionality
The safety valve is designed to open at 8 bar and discharge fluid until the pressure drops below 8 bar. Requires the Network Upgrade Kit (P/N 417501SP). Requires the RFID Upgrade Kit (P/N 417504SP). Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 7: Prepare The System
Note: With solvent and waste monitoring enabled (see page 24), the capacity and current fluid level must be entered Figure 12. Biotage columns up to 50g can be placed on the front of the system. each time you empty a waste reservoir or refill a solvent. -
Page 8: Prime The Solvent Inlets
Flush the System If the automatic line flush is disabled on your system (see page 24), ensure to flush the column flow path before each run (see page 15). Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 9: Set Up A Purification
CV larger than 0.8 L, a safety Figure 16. The run setup view. valve supplied by Biotage (P/N 417115SP) must be used. Specify the Gradient The gradient can be set up using the gradient graph or the gradient table. - Page 10 7. To calculate a purification gradient, press Create. Figure 18. The expanded gradient graph. Gradient markers along the top and right show the length and solvent percentage for the segments. Figure 19. The TLC to Gradient editor. Biotage Selekt Enkel User Manual | © Biotage 2022 ®...
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Page 11: Specify The Information Parameters
CV larger than 0.8 L, a safety detection phase. During this phase, the light absorbance valve supplied by Biotage (P/N 417115SP) must be used. of the used solvents (A and B) is measured for the »... -
Page 12: Specify The Rack Parameters
Rack panel, enable the Max Fraction Volume option (see Figure 23), and enter the desired volume. Note that this setting will only be applied on racks with a higher vessel volume. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 13: Start, Monitor, And Control A Purification
Monitor the Purification in Progress » Read and follow the safety precautions against static electricity While a purification is running, a dynamic chromatogram and in the Biotage Selekt Family Installation and Safety document ® the programmed gradient are displayed in the run view (see (P/N 417511) that is supplied with the system. -
Page 14: Manual Uv Zero
UV Zero: The system is setting the UV zero level using start of a run; see “Rack Sharing” on page 10. the solvent mix used at the start of the gradient. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 15: Manually Fractionate
Start, Monitor, and Control a Purification Empty a Rack Resume the Run For manually selected racks: Before you resume the run, ensure that the setup in the Rack panel corresponds to what is loaded onto the collection bed. » When a rack is removed from the system, it also has to be cleared from the software. -
Page 16: Pause, End, Or Abort A Purification
Gradient Run Completed dialog appears before cleanup is performed, giving the user the possibility to extend the run. The dialog can be minimized by pressing the arrows in the title bar. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 17: Unload A Purification
Unload a Purification Unload a Purification Warning Flush the System » Never flush or purge the system without a column mounted If the last purification of the day is performed with a or the column inlet and outlet tubing coupled together. halogenated solvent (e.g. -
Page 18: Results
The reports for the purifications that have been processed on the system can be accessed by pressing Menu and then Results. If you scan the Biotage columns using the QR reader, the system will be able to trace all runs performed using a specific column The full Selekt Enkel report contains the chromatogram, based on its ID. -
Page 19: Analysis Of The Result
Results Analyze the Spectrum If a Spektra software license is installed, it is possible to switch from viewing the rack(s) to viewing an absorbance spectrum by selecting Spectrum from the View drop-down list (see Figure 39). In this view, you can: »... -
Page 20: Customize The Report
Unit: The length unit. Spektra software license is installed, the spectrum. Note that the full Selekt Enkel report is not overwritten and To add a picture, press To Report in the racks or spectrum view can always be accessed by turning off the Apply Report (see Figure 38 and Figure 41). -
Page 21: Export The Report
1. Select the desired report in the Results view. 2. Select whether to export the full Selekt Enkel report or a customized version by turning the Apply Report Setup option off or on. To customize the report, see page 18. -
Page 22: Flushes And Purge
(see page 5) and flush with at least 30 mL. » Check for leaks in the tubing and fittings. Flow Paths Waste Pump Fraction Collector Bypass S1 S2 Collect Figure 45 . The flow paths in the system. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 23: Air Flush
Flushes and Purge Air Flush Use the air flush feature to e.g. empty a column of remaining solvents after a purification run. 1. Ensure that the correct column is chosen. The column in the current setup will be chosen in the Column Type field. If you want to change this, select the column mounted from the Column Type drop-down menu. -
Page 24: Data Administration
3.1 L. » Never exceed the maximum pressure or flow rate for the column. Note: The preconfigured Biotage column types cannot be edited or deleted. We recommend that you disable Visible for any column that will not be used. -
Page 25: Administrate Racks
(if monitoring is enabled), or Administrate Results a leak is detected by the optional Biotage Solvent Detector. Note: Deleted results cannot be recovered. One, several, or all results can be selected and deleted in the Result Administration view. -
Page 26: System Settings
(Continuous). When limited, the run is paused after 10 CV, enabling the user to decide whether to resume the isocratic hold for up to another 10 CV or end it. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 27: Optional Detectors
To install a license: 1. Create a directory called \biotage\selekt\ on a USB memory device and save the license file to this location. 2. Connect the memory device to a USB port on the left side of the system. -
Page 28: Configure Network Share And E-Mail Functionality
Figure 51. The Network Settings dialog using a static (left) or dynamic This is the network path that you must enter when giving the (right) setup. system access to the share folder; see below. 9. Click Close in the Properties dialog. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... - Page 29 \\server123\Biotage\Selekt where “server123” is the host name, “Biotage” is the name of the share folder, and “Selekt” is a subfolder. In Windows 10, the Network Path can be found by right- clicking the share folder, or the desired subfolder, and selecting Properties from the right-click menu.
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Page 30: Configure An E-Mail Server Connection
6. When you have received a test e-mail, press Save. Note: If you disable the E-mail option, the latest e-mail settings are saved until enabled again. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 31: Maintenance
(if applicable) and press Shut Down. The backup file Export the System Logs to a USB Memory Device is saved on the USB memory device at biotage\selekt\ 1. Connect the USB memory device to a USB port on the left Selekt_ serialnumber \backups\ or in the network share side of the system. -
Page 32: Calibrate The Uv Detector
. The flow cell of the UV detector. A = flow cell outlet tube, After cleaning, wipe dry with a clean, non-abrasive cloth. B = flow cell, C = flow cell inlet tube, and D = retaining nut. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 33: Monthly Maintenance Of The Flow Cell
Maintenance Monthly Maintenance of the Flow Cell 4. Flush the flow cell with a series of miscible solvents or a detergent solution (see “Wash solution” below) using the Perform the following flush procedure as part of your scheduled injection maintenance kit supplied with the system. preventive maintenance. -
Page 34: Leaks
» Shut down the system before replacing any tubing. Use only tubing designed for the Selekt Enkel system and supplied by Biotage. Shut Down the System at Leakage If a leakage is observed, shut down the system as follows: Figure 60. - Page 35 Ensure that the secondary solvent containment and solvent detector (including the space underneath the c. Contact Biotage 1-Point Support. detector) are cleaned and wiped dry. To remove the Internal Leakage solvent detector, remove the cable locking plate, open the Internal leakage, due to e.g.
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Page 36: Replace The Fuses
B in Figure 67. Figure 67 . Assemble the needle, ferrule (A), and nut, and assemble it on the collection arm. Ensure that the needle is touching the needle guide (B). Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 37: Troubleshooting
Ensure that there is nothing obstructing or restricting the arm movement. If this does not solve the problem, the collection arm may need to be recalibrated. Contact Biotage 1-Point Support. Longer delay than expected (approx. 2 CV instead of 1 CV) »... -
Page 38: Leak Detected
Warning » Shut down the system before replacing any tubing. Use only tubing designed for the Selekt Enkel system and supplied by Biotage. Figure 69. Removing the check valve cap from one of the CV OUT valves. 1. Once the pressure has decreased to ambient pressure (the... -
Page 39: Qr Reader Problems
Biotage 1-Point Support. Contact Biotage 1-Point Support ® ™ For assistance at any time during troubleshooting or if your problem persists, contact Biotage 1-Point Support. See contact information on the back of this document or visit our website www.biotage.com. -
Page 40: Solvent Specifications
Dean J. A. Lange’s Handbook of Chemistry, 15th edition, McGraw-Hill (1999). Snyder L. R. and Kirkland J. J. Introduction to Modern Liquid Chromatography, Wiley (1979). When water is used in reversed phase chromatography, the strength value is 0. Biotage Selekt Enkel User Manual | © Biotage 2022 ®... -
Page 41: General Information
Mains US Cable 416225SP 480 mL Flask RFID Rack (holds 8 flasks) 416321SP Biotage Selekt Secondary Solvent Containment ® For a complete list, please visit our website www.biotage.com. with Biotage Solvent Detector ® 416410SP Biotage Selekt Instrument Tray with Biotage ®... -
Page 42: Notes
Notes Notes Biotage Selekt Enkel User Manual | © Biotage 2022 ®... - Page 44 Part Number: 417510-C © 2022 Biotage. All rights reserved. No material may be reproduced or published without the written permission of Biotage. Information in this document is subject to change without notice and does not represent any commitment from Biotage. E&OE.
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