Biotage Isolera Prime User Manual
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Biotage Isolera Prime User Manual

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Summary of Contents for Biotage Isolera Prime

  • Page 1 Isolera User Manual...
  • Page 2 Copyright Biotage Sweden AB reserves the right to make changes to the information contained herein without prior notice. No part of this manual may be reproduced or transmitted in any form or by any means, electronic or mechanical, for any purpose, without the expressed written permission of Biotage Sweden AB.

  • Page 3: Table Of Contents

    Table of Contents 1: Description and Specifications - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1-1 General System Description - - - - - - - - - - - - - - - - - - - - - - - - - - 1-1 Basic System Components - - - - - - - - - - - - - - - - - - - - - - - - - 1-2...
  • Page 4 Administrate the Solvent List - - - - - - - - - - - - - - - - - - - - - - - - - - 2-8 Add a Solvent - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2-8 Delete an Unused Solvent - - - - - - - - - - - - - - - - - - - - - - - - - 2-8 Edit a Solvent - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2-9...
  • Page 5 5: Maintenance - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 5-1 Contact Biotage 1-Point Support - - - - - - - - - - - - - - - - - - - - - - - - - 5-1...
  • Page 6 Manufacturer - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 7-1 Biotage Sweden AB - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 7-1...

  • Page 7: 1: Description And Specifications

    Biotage Leak Detector Touch Screen Cartridge Holder Power Switch SNAP Cartridge Collection Arm Leak Detector (optional on Isolera Prime, One, and Four and standard on Isolera LS) Collection Rack Leak Tray (optional) Collection Tray Sample Loading Pump (only on Isolera LS) Solvent Tray Figure 1-1.

  • Page 8: Basic System Components

    1.1.2 System Configurations There are four system platforms available: Biotage Isolera Prime™, Biotage Isolera™ One, Biotage Isolera™ Four, and Biotage Isolera™ LS (Large Scale). The differences are listed in the table below. Table 1: System Differences Isolera Prime* Isolera One*...

  • Page 9: Programmed Functions

    Back: Return to the last help topic that you viewed. Close: Exit the help. 1.1.5 Accessories Cartridges The Isolera system is designed to operate with Biotage SNAP™, Biotage SNAP Ultra™ and Biotage ® ® ZIP™ cartridges, which have easy-to-use Luer-lok™ connections. FLASH+ and ISOLUTE cartridges can also be used but may require tube adapters and a separate ring stand.
  • Page 10 Figure 1-2. SNAP 100g Cartridge and Samplets Note that SNAP and SNAP Ultra 10g to 30g and Biotage ZIP 5g to 45g cannot be used on Isolera LS, and SNAP 1500g is not recommended for use on Isolera Prime, Isolera One, or Isolera Four. SNAP 750g, Flash 75S, Flash 75M, and Flash 75L are not recommended for use on Isolera Prime.
  • Page 11 Sample Loading Methods for Biotage ZIP Cartridges Liquid samples can be injected into the Biotage ZIP cartridge inlet Luer fitting using a syringe or, when using an Isolera LS system, the sample loading pump. Dried, preadsorbed sample on media can be loaded externally using a dry load vessel (DLV-030 or DLV-070).
  • Page 12 The maximum collection volume for Isolera LS can be increased from 9.6 liters up to 320 liters by using a funnel rack kit from Biotage. The funnel rack kit comes with two racks (16 positions each) and a cart with wheels that holds the Isolera LS system and the collection vessels. For more information, please contact your local representative.

  • Page 13: Software Description

    1.2 Software Description 1.2.1 Main Menu The system’s main menu provides access to the three different software modes: • Chemistry: Set up, control, monitor, and review a purification. • Data Administration: Administrate cartridge types, methods, rack types, results, solvents, and user accounts. •...

  • Page 14: System Mode

    In the System mode, the software is divided into six (6) tabs that can be accessed in the right-hand panel (see Figure 1-6). Here is a short description of what you can do at each tab: • Detector: Enable or disable the internal detector, an external detector (e.g. Biotage ELSD- 1080), and Biotage Leak Detector. •...

  • Page 15: Chemistry Mode

    Purifications can be queued up when using an Isolera Four system. † The Air Flush feature is not available when using an Isolera Prime system. Task field Figure 1-7. Chemistry Mode (Isolera LS and Isolera Four EXP with Spektra License Shown)
  • Page 16 (in the System mode) and a system decompression process (Purge). It is also possible to manually instruct the system to perform an Air Flush and a Purge. Note that the Air Flush feature is not available with Isolera Prime. •...

  • Page 17: Method Tab (Chemistry Mode)

    Base the new method on an existing method. You can either base the method on one of the preconfigured Biotage methods or a method created and saved in the system’s database, on a USB memory device, or in a share folder on the network. After editing, you can save the method under a new name by entering a unique method name.
  • Page 18 • Monitor and edit a purification in progress. While a purification is running, the programmed gradient and a dynamic chromatogram are displayed. The chromatogram can be magnified, dragged to the desired position, etc. If a Spektra license has been installed on your system, the absorbance spectrum for the whole detector range can be viewed in the gradient view by pressing the Show λ...
  • Page 19 The gradient graph shows the defined gradient (excluding the additive*). The actual percentage of pumped solvents (including the additive*, if used) are displayed underneath the graph. Not available when using an Isolera Prime system. 411829-L, Description and Specifications April 2012...

  • Page 20: Results Tab (Chemistry Mode)

    1.2.7 Results Tab (Chemistry Mode) Purifications that are processed on the system are stored as individual records in the system’s database. The records can, if desired, be saved by the user as XML files (text files including all raw data) on a USB memory device or in a share folder on the network. At the Results tab, in the Chemistry mode, it is possible to search and view the results of all purifications stored in the system’s database, on a USB memory device (if connected), or in a share folder on your network (if connected).

  • Page 21: Solvents Tab (Chemistry Mode)

    • Flush a cartridge with air to empty it of remaining solvents before unloading it. This feature is not available with Isolera Prime. • Purge a cartridge to release any remaining pressure before unloading it.

  • Page 22: Isolera Remote Viewer

    1.2.9 Isolera Remote Viewer With the system connected to your network, it is possible to perform the following tasks through a standard web browser (through the Isolera Remote Viewer feature): • Create a new method. If desired, the method can be based on a previous purification found in the Results view.

  • Page 23: Specifications

    1.3 Specifications 1.3.1 General System Specifications Power Input Voltage 100-240 VAC, 50/60 Hz Fuses 4.0 TA/250 V, P/N 411916 (2 required) Max Power Consumed 200 VA Certification Certificates CE, UL, CAN/CSA Directives LVD, Directive 2006/95/EC EMC, Directive 2004/108/EC WEEE, Directive 2002/96/EC Standards EN 61010-1: 2001 EN 1127-1:2007...

  • Page 24: Operational Performance Specifications

    Solvents Solvent Supply A maximum of 2 x 4 liter (Isolera Prime) or 4 x 4-liter (Isolera One, Four, and LS) non- glass reservoirs on top of the system (the solvent tray). Larger reservoirs can be placed on the side of the system. If using glass reservoirs, up to 2.5-liter reservoirs can be placed on the solvent tray.

  • Page 25: Solvent Specifications

    1.3.3 Solvent Specifications WARNING Many solvents are considered to be hazardous to humans and the environment, so take appropriate safety precautions when using them. Comply with Material Safety Data Sheets (MSDS) and any other applicable regulations for the safe use, handling, transporting, storage, and disposal of these solvents.

  • Page 26: Chemical Resistance Of The Peristaltic Pump Tube (Isolera Ls)

    1.3.4 Chemical Resistance of the Peristaltic Pump Tube (Isolera LS) The Isolera LS system is delivered with two kinds of peristaltic pump tubes. Ensure that the used pump tube is compatible with the solvents and sample to be used. NOTE The peristaltic pump tube has limited use.

  • Page 27: 2: Data Administration

    Chapter Data Administration (Data Administration Mode) WARNING Before performing any procedures in this chapter, please read and observe the safety requirements in the “Isolera Installation and Safety” document (P/N 411828). Failure to follow those requirements may result in personal injury and/or equipment damage. 2.1 Log into the Data Administration Mode You must log into the Data Administration mode to be able to administrate cartridge types, methods, rack types, results, solvents, and user accounts.

  • Page 28: Administrate The Cartridge List

    NOTE The preconfigured Biotage cartridges cannot be edited or deleted. SNAP and SNAP Ultra 10g to 30g and Biotage ZIP 5g to 45g cannot be used on Isolera LS. SNAP 1500g is not recommended for use on Isolera Prime, Isolera One, or Isolera Four. SNAP 750g, Flash 75S, Flash 75M, and Flash 75L are not recommended for use on Isolera Prime.

  • Page 29: Edit A Cartridge Type

    (If the user wants to base a new method on a method that is not set as a default method, the user will have to browse for the method.) NOTE The preconfigured Biotage methods cannot be exported or deleted. To scroll a list down or up, press Figure 2-3. Methods Tab (Isolera LS Shown) 2.3.1...

  • Page 30: Export Methods

    (if file share has been set up; see page 3-7). The method(s) to import must be available at “USB”:\biotage\isolera\methods or “share folder”\biotage\isolera\methods. 1. If you want to import the method(s) to a certain user, select the user at the Methods tab.

  • Page 31: Administrate The Rack List

    NOTE The preconfigured Biotage racks cannot be edited or deleted. The funnel rack kit from Biotage can only be used with Isolera LS. To scroll a list down or up, press 2.4.1 Add a Rack Type 1.

  • Page 32: Delete An Unused Rack Type

    2.4.2 Delete an Unused Rack Type 1. Select the rack that you want to delete at the Racks tab. 2. Press Delete. The Confirm Delete dialog opens. 3. To confirm delete, press Yes. 2.4.3 Edit a Rack Type 1. Select the rack that you want to edit at the Racks tab. 2.

  • Page 33: Export And Delete Result Records

    2.5.1 Export and Delete Result Records You can either export a single or all listed records to a USB memory device or to a share folder on your network (if file share has been set up; see page 3-7). The records can be exported as CSV files (text files including all detector data), PDF files, or XML files (text files including all raw data).

  • Page 34: Administrate The Solvent List

    The maximum speed at which the solvent can be drawn into the pump during the fill stroke, in ml/min. The valid range is 1 to 200 ml/min when using an Isolera Prime, Isolera One or Isolera Four system and 50 to 500 ml/min when using an Isolera LS system. If this parameter is turned off, the system’s maximum fill rate will be used (i.e.

  • Page 35: Edit A Solvent

    (if monitoring is enabled), a waste reservoir needs to be emptied (if monitoring is enabled), and a leak is detected (if using Biotage Leak Detector). Privilege: A user can have chemist or system owner privilege: •...

  • Page 36: Change The Password Or Privilege

    Figure 2-8. Users Tab 2.7.2 Change the Password or Privilege 1. Select the user account that you want to edit at the Users tab. 2. Press Edit..The Edit User dialog opens. 3. Edit the user settings. (A setting can be entered when the corresponding text box has been selected.) If you change the user name, all methods and result records associated with the user will be updated.

  • Page 37: 3: System Settings

    Chapter System Settings (System Mode) WARNING Before performing any procedures in this chapter, please read and observe the safety requirements in the “Isolera Installation and Safety” document (P/N 411828). Failure to follow those requirements may result in personal injury and/or equipment damage. 3.1 Log into the System Mode You must log into the System mode to be able to change detector, network, reporting, and runtime settings, set the system clock, calibrate the touch screen, fraction collector, and internal detector,...

  • Page 38: Change Detector Settings

    NOTE The external detector and the tube assembly must be able to handle a maximum dispense rate of 200 ml/min (when using an Isolera Prime, Isolera One, or Isolera Four system) or 500 ml/min (when using an Isolera LS system) and a maximum pressure of 10 bar.

  • Page 39: Disable An External Detector

    The valid range is 1 to 200 ml/min when using an Isolera Prime, Isolera One, or Isolera Four system and 50 to 500 ml/min when using an Isolera LS system. If this parameter is turned off, the system's maximum dispense rate will be used (i.e.

  • Page 40: Change General Settings

    To disable Biotage Leak Detector: 1. Select the Detector tab in the right-hand panel. 2. Select the Enabled text box in the Leak Detector field and select No. 3.3 Change General Settings 3.3.1 Set the Date and Time Setting the date and time correctly ensures an accurate date and time stamp for your result records and can help you search for specific records.

  • Page 41: Enable The Λ-All Detection Mode And Tlc To Step Gradient Editor

    To install a Spektra license for the λ-all detection mode and the TLC to Step Gradient editor: 1. Save the Spektra license file in a “biotage/isolera/” directory on a USB memory device. 2. Connect the memory device to the USB port located underneath the touch screen.

  • Page 42: Change Report Settings

    Figure 3-6. Network Tab To configure the network connection: 1. Connect a shielded category 5 TP cable to the ETHERNET port. 2. Select the Network tab in the right-hand panel. 3. Select the Network Type text box and select Dynamic or Static. 4.

  • Page 43: Set Up File Sharing And Auto Save Of Reports

    5. Select the Printer Model text box. The Printer Setup Wizard opens. 6. Read and follow the instructions that appear on the screen. Tip! If the Isolera system does not have a matching printer driver for the connected printer, select Generic in the wizard’s manufacturer list and check if there is a printer protocol available that is supported by your printer (check the printer user manual).

  • Page 44: Set Up Auto Send Of Reports

    If the Audible Alarm option is enabled, an audible warning will sound in the following situations: 1) rack change is needed, 2) leakage (if using Biotage Leak Detector), 3) high pressure, 4) low solvent volume (if solvent monitoring is enabled), 5) full waste reservoir (if waste monitoring is...

  • Page 45: Enable Or Disable Peak Mode

    • Automatic Vessel: The system allocates vessels in sequence, starting with the first vessel in rack A, the second in rack A, and so forth. With this rack allocation method, racks can be shared by several runs but the collection tray cannot be shared by several users. When enabling automatic rack allocation on an Isolera Four system, the following rules also apply: •...

  • Page 46: Enable Or Disable Run Requirement Estimation

    Line Flush: The system flushes the inlet line and the cartridge with 9 ml (when using an Isolera Prime, Isolera One, or Isolera Four system) or 20 ml (when using an Isolera LS system) of the specified solvent or solvent mix.

  • Page 47: Configure Collection And Fractionation

    3. If you want to save the system configuration and logs in the specified share folder, press Export in the System Configuration and Logs field. 4. The system configuration and logs are saved as a zip file at \biotage\isolera\logs\. Send the zip file to 1-Point Support; see “Contact Information” on page 7-1.

  • Page 48: Back Up And Restore The System's Database

    Press Restore in the Back Up and Restore Database field. 3. If the backup is available in the specified share folder (at \biotage\isolera\backup\), press Restore in the Back Up and Restore Database field. 4. In the Select Backup File dialog, select the backup file and press OK. The Restore from USB Device/Share Folder dialog opens.

  • Page 49: Calibrate The Fraction Collector

    Figure 3-9. Maintenance Tab 3.7.5 Calibrate the Fraction Collector If the fraction collector needs to be recalibrated, use the following procedure. 1. Select the Maintenance tab in the right-hand panel. 2. Press Calibrate in the Fraction Collector field. Read and follow the instructions that appear on the screen.

  • Page 50: Release Stuck Check Valves

    3.7.9 Release Stuck Check Valves Low or inconsistent flow delivery volume and/or superimposed periodic UV or UV-VIS signals can be signs of sticking check valves. Use the following procedure to release stuck check valves. 1. Select the Maintenance tab in the right-hand panel. 2.

  • Page 51: 4: Operation

    Chapter Operation (Chemistry Mode) WARNING Before performing any procedures in this chapter, please read and observe the safety requirements in the “Isolera Installation and Safety” document (P/N 411828). Failure to follow those requirements may result in personal injury and/or equipment damage. 4.1 Start Up the System It is recommended that you start up the system in the following order: 1.

  • Page 52: Control The Uv Lamp

    Open Method dialog. All methods of the selected user are listed. To select one of the preconfigured Biotage methods, select the user “Biotage Methods”. c. If the method is stored on a USB memory device, connect the device to the USB port located underneath the touch screen and press in the Open Method dialog.
  • Page 53 If you want to calculate the gradient from TLC data, see page 4-10. Isolera One, Four, and LS have four solvent inlets (A, B, C, and D) while Isolera Prime has two (A and B). 1. Select the Gradient tab.
  • Page 54 Data Administration mode, • the maximum dispense rate of the system (200 ml/min for Isolera Prime, One and Four, and 500 ml/min for Isolera LS) or, if using an external detector, the maximum dispense rate defined for the external detector in the System mode.
  • Page 55 As a single-piston pump only delivers liquid during the dispense stroke, the maximum flow rate when using an Isolera Prime system is equal to (Max Fill Rate x Max Dispense Rate)/(Max Fill Rate + Max Dispense Rate). Examples: If the fill rates for the used solvents are 50 ml/min and 80 ml/min and you use a external detector with the maximum dispense rate of 100 ml/min, the highest flow rate that can be used is (50x100)/(50+100)=33 ml/min.
  • Page 56 The maximum collection volume for Isolera LS can be increased from 9.6 liters up to 320 liters by using a funnel rack kit from Biotage. The funnel rack kit comes with two racks (16 positions each) and a cart with wheels that holds the Isolera LS system and the collection vessels. For more information, please contact your local representative.
  • Page 57 411829-L, Operation April 2012 Page 4-7...
  • Page 58 • External: Control the collection and fractionation using an external detector (e.g. an Biotage ELSD-1080 detector). Possible collection and fractionation parameters are Collect All and Start Threshold. 3. If you want the system to perform baseline correction, turn on the Baseline Correction option.
  • Page 59 5. Specify the collection and fractionation settings to be used in the Parameters field. Refer to Table 4-2 below for guidance. For more information; see the “Collection and Fractionation” appendix on page A-1. Table 4-2: Collection and Fractionation Parameters Parameter Description Start The shortest wavelength to be included in the λ-all signal.

  • Page 60: Calculate Gradient From Tlc Data

    4.4.2 Calculate Gradient from TLC Data Use the TLC to Linear Gradient editor or the TLC to Step Gradient editor (requires a Spektra license) to calculate a purification gradient and get cartridge and sample load recommendations. TLC to Linear Gradient The software’s TLC to Linear Gradient editor is used to enter the following parameters obtained from a TLC procedure: •...
  • Page 61 TLC to Step Gradient The software’s TLC to Step Gradient editor is used to enter the following parameters: • The R value for each compound of interest, for each TLC plate. • The percentage of the strongest solvent in the TLC solution, for each TLC plate. •...
  • Page 62 after. If no peak of interest has been set, the load capacity for each cartridge type is calculated according to the smallest CV. 12. To close the TLC to Step Gradient editor and open a method containing the purification gradient at the Method tab, press OK.

  • Page 63: Create A Method Using The Method Wizard

    Linear gradients can be automatically optimized by converting them to step gradients which reduces solvent use. NOTE Gradients that are optimized on an Isolera Prime, Isolera One, or Isolera Four system cannot be run on an Isolera LS system due to the different system volumes used for the calculation, and vice versa.

  • Page 64: Create A Method Using A Web Browser

    Select the curve to base the optimization on ( ) Press to toggle between showing ( ) and hiding the original gradient Use this slider Use this slider to adjust the to adjust the peak sensitivity threshold Original gradient Optimized step gradient Figure 4-9.

  • Page 65: Prepare And Run A Purification

    4.5 Prepare and Run a Purification 4.5.1 Prepare the System for a Run 1. If no equilibration is to be performed and an internal dry loading technique is to be used (see page 1-4), load the sample. See Table 4-3 on page 4-21 for cartridge loading capacity. 2.
  • Page 66 Figure 4-10. Tube Connections 411829-L, Operation April 2012 Page 4-16...
  • Page 67 Prepare the Sample Loading Pump for a Run (Only Isolera LS) NOTE The peristaltic pump tube has limited use. To avoid the risk of cross- contamination and decreased pump performance, it is recommended that the peristaltic pump tube is discarded after each run. The system is delivered with two kinds of peristaltic pump tubes (PharMed and Fluran).
  • Page 68 To reuse the peristaltic pump tube used in the previous run (not recommended – see the note above), flush and fill the tubing with a solvent suitable for the next purification: 1. Place the pump’s inlet line into the solvent of your choice. 2.

  • Page 69: Run A Purification

    4.5.2 Run a Purification WARNING If you at any time during a purification need to pause the system, press the Pause button in the right-hand panel (in the software). The collection arm returns to its home position (the inner right corner) and the system is paused.
  • Page 70 NOTE When using an Isolera Four system, queued equilibrations take priority over queued gradient runs. Isolera One EXP and Isolera LS Rack positions for 120 ml racks: Isolera Four EXP Rack positions for 16x100 mm racks: Figure 4-15. Run Parameters and Estimation Dialogs Task field Figure 4-16.
  • Page 71 FLASH 75L Cannot be used on Isolera LS. † When using Isolera Prime, the maximum flowrate is limited to 100 ml/min. When using Isolera One or Isolera Four, the maximum flow rate is limited to 200 ml/min. ‡ Not recommended for use on Isolera Prime, Isolera One, or Isolera Four.

  • Page 72: Change The Processing Order

    “Specify How Flushes Are Performed” on page 3-10. Note that the Air Flush feature is not available with Isolera Prime. 4.6 Change the Processing Order When using an Isolera Four system, it is possible to change the processing order.

  • Page 73: Monitor And Control A Purification

    4.7 Monitor and Control a Purification 4.7.1 Monitor the Purification in Progress Select the Status tab in the right-hand panel. While a purification is running, one graph displays the programmed gradient and the other a dynamic chromatogram. If a Spektra license has been installed on your system, an absorbance spectrum for the whole detector range can be viewed in the gradient view by pressing the Show λ...

  • Page 74: Collect Through The Waste Channel

    4.7.4 Collect Through the Waste Channel If you want to start collecting fractions through the waste channel, press the Bypass Tray button at the Status tab during the gradient run ( = collection through waste is enabled). You will be requested to connect a collection vessel to the waste channel and enter the max collection volume of the vessel.

  • Page 75: Pause, End, Or Abort A Purification

    5. If you extended a finished run or edited a gradient run that was not started or queued up*, press Gradient to start (or queue up*) the run. Note that if you extend a run on an Isolera Four system and UV Zero has been performed on another cartridge in between, the system will perform a UV Zero.

  • Page 76: Unload A Purification

    Solvents tab. See “Flush a Cartridge with Air” below and “Purge a Cartridge” on page 4-27. Note that the Air Flush feature is not available with Isolera Prime. 2. If using an Isolera Four system and the system is processing, press Pause.

  • Page 77: Purge A Cartridge

    To manually instruct the system to perform an air flush: 1. Select the Solvents tab in the right-hand panel. 2. Select the Air Flush tab. 3. If using an Isolera Four system, select the Channel text box and select the cartridge to be flushed and the waste reservoir to be used.

  • Page 78: Access Result Records

    4.9 Access Result Records 4.9.1 Search for Records Purifications that are processed on the system are stored as individual records in the system’s database. Possible search criteria for these records are 1) user name, 2) project name, 3) method name, 4) sample name, and 5) date when the purification was run. To search for records in the system’s database: 1.

  • Page 79: View, Print, And Save Reports On A Usb Memory Device Or The Network

    4.9.2 View, Print, and Save Reports on a USB Memory Device or the Network Two result reports are available for each purification: 1) an archive report with purification details, a chromatogram, a 3D absorbance spectrum for the whole detector range (requires a Spektra license), and TLC data (if entered in the TLC to Step Gradient editor) and 2) a fraction report.
  • Page 80 7. To save the selected report as a PDF file in a share folder on your network, press Save. The report is saved at “share folder”\biotage\isolera\results\. (If the button is disabled, no file sharing has been set up. See “Set Up File Sharing and Auto Save of Reports” on page 3-7.)

  • Page 81: Save Records On A Usb Memory Device Or The Network

    Figure 4-25. Report Setup Tab Color Legend The colors used at the Report Setup tab are: = The vessel has not been used for the viewed purification. = The vessel is deselected. = The vessel is selected and contains flush liquid. , and = The vessel is selected and contains a fraction.

  • Page 82: Solvent And Waste Handling

    4.11 Solvent and Waste Handling 4.11.1 Assign Solvents to the Solvent Inlets When a purification is run, the software references the solvent assignments to determine which solvent inlets that are connected to the solvents used in the method. 1. Select the Solvents tab in the right-hand panel. 2.

  • Page 83: Prime The System

    If you want to fill a solvent inlet with a new solvent, we recommend that you prime with at least 40 ml (when using an Isolera Prime, Isolera One, or Isolera Four system) or 50 ml (when using an Isolera LS system) of that solvent. If you also want to fill the liquid lines from the pump to...
  • Page 84 (selected*) waste valve with the same solvent, we recommend that you prime with at least 60 ml when using an Isolera Prime, Isolera One, or Isolera Four system or 100 ml when using an Isolera LS system. 9. Select the Flowrate text box and enter the flow rate. The maximum flow rate that can be used depends on: •...

  • Page 85: Access The Isolera Remote Viewer

    4.12 Access the Isolera Remote Viewer With the system connected to your network, it is possible to perform the following tasks through a standard web browser (through the Isolera Remote Viewer feature): • Create a new method. If desired, the method can be based on a previous purification found in the Results view.

  • Page 86: Contact Biotage 1-Point Support

    Biotage 1-Point Support immediately. 5.1 Contact Biotage 1-Point Support If your system has been damaged and does not function properly, shut it down and contact Biotage 1-Point Support. See contact details on page 7-1. 5.2 Accessories To order cartridges, please see the Biotage Product Catalog.

  • Page 87: Isolera Prime, Isolera One, And Isolera Four

    Leak detector for systems with one collection tray (Prime, One, and Four 412019 systems) Leak detector for systems with two collection trays (EXP and LS systems) 412062 5.2.2 Isolera Prime, Isolera One, and Isolera Four Accessory Quantity Part Number Test tube rack, 13 x 100 mm 411789...

  • Page 88: Spare Parts

    5.3 Spare Parts A list of spare parts is available at www.biotage.com. 5.4 Clean the Exterior of the System Regular cleaning of the touch screen, if performed properly, extends the touch screen life and protects it from contaminants that cause unnecessary wear. Always turn the system off before cleaning the screen.

  • Page 89: Clean Or Release Valves

    7. Ensure that the cartridge’s inlet and outlet tubing are coupled together. 8. Select the Volume text box and enter the total flush volume. We recommend that you flush with at least 30 ml (Isolera Prime), 60 ml (Isolera One and Isolera Four), or 100 ml (Isolera LS). 411829-L, Maintenance...

  • Page 90: Release Stuck Check Valves

    9. Select the Flowrate text box and set the flow rate to 100 ml/min (Isolera Prime), 200 ml/min (Isolera One and Isolera Four) or 500 ml/min (Isolera LS). 10. Enter 100 percentage for the solvent inlet line used for methanol. Enter “0” or press Clear for the other solvents that are connected.

  • Page 91: Replace The Sample Loading Pump Tubing

    (sonicated) or replaced every 1000 hours of operation or every 12 months, whichever comes first (P/N 412720 for Isolera Prime, One, and Four systems and P/N 412628 for Isolera LS systems). If using a system where the filers are not screwed onto the solvent inlets lines, we recommend that you replace both the inlet lines and the filters (P/N 413008 for Isolera One and Isolera Four, and P/N 413017 for Isolera LS, both kits include four inlets lines and four filers).

  • Page 92: Leaks

    4. If an air flush (started by the user) is in progress, press End Air Flush at the Solvents tab. The Air Flush feature is not available with Isolera Prime. 5. If using an Isolera Four system, remove any queued purifications at the Status tab by pressing Remove.

  • Page 93: External Leakage

    2. Disconnect the power cord from the power outlet. 3. Remove the spillage using the appropriate safety precautions. 4. If using Biotage Leak Detector, ensure that the leak detector and the leak tray (including the space underneath the detector) is cleaned and wiped dry.

  • Page 94: Assembling Tubes Correctly

    4-33. Prime with water or another suitable solvent. 9. If the problem persists, press End Prime, shut down the system (see page 4-1), disconnect the power cord from the power outlet, and contact Biotage 1-Point Support (see contact details on page 7-1).

  • Page 95: Replace The Fuses

    The system uses double-pole fusing (both sides of the line are fused). Fire hazard; always replace with the same type and rate fuses only. If the system is damaged or does not function properly, contact Biotage 1-Point Support for repair information (see contact details on page 7-1).

  • Page 96: Replace The Detector Lamp(S)

    4. Mount the needle on the collection arm. Ensure that the needle is touching the needle guide. Mount the needle here The needle should touch the needle guide Figure 5-11. Mount the Needle on the Collection Arm 5.13 Replace the Detector Lamp(s) 1.
  • Page 97 7. Pull out the UV lamp and disconnect it from the power cable; see Figure 5-14. Press here and disconnect the UV lamp Figure 5-14. Disconnect the UV Lamp NOTE Do no touch the UV lamp glass. 8. Mount a new UV lamp (P/N 09830 for all internal Isolera detectors except UV-VIS and P/N 412970 for UV-VIS).

  • Page 98: Contact Biotage 1-Point Support

    Biotage 1-Point Support immediately. 6.1 Contact Biotage 1-Point Support If your problem persists, or for assistance at any time during troubleshooting, contact Biotage 1-Point Support. See contact details on page 7-1. 6.2 Accessories and Spare Parts A condensed list of accessories is available on page 5-1.

  • Page 99: Pump-Related Problems

    Re-circulating the solvent is not recommended. Particulate-free solvent is required. If the problem persists, the pump piston seals or the check valve seals may be worn. Please contact Biotage 1-Point Support; see contact details on page 7-1. 2. Low or inconsistent a.

  • Page 100: Internal Detector-Related Problems

    UV Zero. c. Sample in the flow Retry performing the UV Zero when there is no sample cell. in the flow cell. If the problem persists, please contact Biotage 1-Point Support (see page 7-1). 411829-L, Troubleshooting April 2012 Page 6-3...

  • Page 101: Gradient Problems

    6.6 Gradient Problems Problem Solutions Low composition When generating solvent mixtures where the composition contains less than 10% gradient is not correct. of one solvent: 1. Ensure that the solvent with the lowest percentage is pumped first, i.e. is mounted on a solvent inlet with a lower number than the solvent with the highest percentage.

  • Page 102: Leak Detected

    6.7 Leak Detected See “Leaks” on page 5-7. 6.8 Overpressure Detected Possible Causes Solutions 1. Blockage due to kinked a. Press Purge... in the alarm dialog. tubing. b. When the pressure has been reduced to an acceptable level, press End in the Purge dialog.

  • Page 103: Manufacturer

    Sales Offices and Distributors Please visit www.biotage.com for contact information. Technical Support Call, fax, or e-mail Biotage 1-Point Support, or request support online (www.biotage.com). When contacting 1-Point Support, please have the following information ready: • Company name and contact information.

  • Page 104: A: Collection And Fractionation

    UV detector with the range 200-400 nm, or a UV-VIS detector with the range 200-800 nm. (The UV-VIS detector is not available with Isolera Prime and the fixed UV detector is not available with Isolera LS.) It is also possible to connect an external detector to the system, e.g. an Biotage ELSD-1080 detector.

  • Page 105: Possible Combinations

    4. Manual: The collection is started and stopped by the user. Fractionation only occurs on volume. • External detector: The system uses the signal from the external detector for collection only. A.1.4 Possible Combinations The possible combinations of collection and fractionation methods are listed in Table A-1. Table A-1: Possible Combinations of Methods Fractionation Method Collection...

  • Page 106: Collection And Fractionation Parameters

    It is possible to combine the Collect All with the Threshold and/or the Slope collection method. This means that everything will be collected and fractionation will occur on the Start Threshold level and/or the Start Slope setting. Fractionation will also occur on detected valleys as well as on shoulders, if enabled.
  • Page 107 Table A-2: Method Parameters Parameter Description Start Slope The value of the collect start slope. The range is [Off | 1 to 5000 mAU/CV]. (Only available with the UV1 and UV1+UV2 detection modes.) Shoulder Slope The value of the fractionation shoulder slope. The range is [Off | 1 to 5000 mAU/CV]. (Only available with the UV1 and UV1+UV2 detection modes.) System Parameters The system parameters are defined for all methods and can be changed (in the System mode) only...

  • Page 108: Shoulder Slope And Valley Fractionation

    A.2 Shoulder Slope and Valley Fractionation A.2.1 Fractionation Using the Shoulder Slope Parameter When the Shoulder Slope parameter is enabled (the range is 1 to 5000 mAU/CV), the Isolera system fractionates on rising and falling shoulders and double fractionates on valleys. Figure A-1.
  • Page 109 Figure A-2. Fractionation on Falling Shoulder Figure A-3. Double Fractionation on Valley 411829-L, Collection and Fractionation April 2012 Page A-6...

  • Page 110: Fractionation Using The Valley Slope Parameter

    A.2.2 Fractionation Using the Valley Slope Parameter When the Valley Slope parameter is enabled (the range is 1 to 5000 mAU/CV) while the Shoulder Slope parameter is disabled (Off), the Isolera system only fractionates on valleys. Figure A-4. Single Fractionation on Valley 411829-L, Collection and Fractionation April 2012 Page A-7...

  • Page 111: Collection And Fractionation Examples

    A.3 Collection and Fractionation Examples A.3.1 Threshold Collection A.3.2 Slope Collection 411829-L, Collection and Fractionation April 2012 Page A-8...

  • Page 112: Slope Collection And Threshold Fractionation

    A.3.3 Slope Collection and Threshold Fractionation A.3.4 Valley Fractionation 411829-L, Collection and Fractionation April 2012 Page A-9...

  • Page 113: Shoulder Slope Fractionation

    A.3.5 Shoulder Slope Fractionation A.4 Auto Extend of Gradient When the system enters the Auto-Extend mode, the gradient is extended with an isocratic segment that is 25% of the total gradient length using the end solvent mix of the current gradient. The criteria for deciding if the gradient should be extended depend on the collection method and the parameters defined for the collection method: Collection Method...
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  • Page 116 Support Order Distributors North America Please visit our website North America Phone: +1 800 446 4752 press 3 Phone: +1 704 654 4900 www.biotage.com Outside US: +1 704 654 4900 Fax: +1 704 654 4917 for contact details. ordermailbox@biotage.com Fax: +1 704 654 4917 US-1-pointsupport@biotage.com...

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