Nikon ECLIPSE Ei Instructions Manual page 51

10
Adjust the diopter.
Swing the 40x objective in the optical path and rotate
the focus knobs to focus on the specimen.
Then, change the objective to the 10x (or 4x) objective
and focus on the specimen by rotating the diopter ring
without using the focus knob. Perform this procedure
while looking into the right eyepiece with your right eye
and the left eyepiece with your left eye. → Section 3.4,
"Adjustment of Diopter"
11
Change the objective to the magnification
used for observation.
Rotate the revolving nosepiece to bring the objective
of the desired magnification into the optical path.
Do not directly switch from the 4x objective to
the 60x or 100x objective. Be sure to focus on
the specimen with the 10x objective before
using the objective of a higher magnification.
12
Adjust the aperture diaphragm.
Set the aperture diaphragm lever to the adjustment
position mark of aperture diaphragm (the same figure
as the magnification of the objective). → Section 3.5,
"Adjustment of Aperture Diaphragm"
13
Observe the specimen.
When you need to observe the specimen with a
different magnification, repeat steps 11 and 12.
14
Turn off the microscope.
Set the light ON/OFF switch to OFF (the status display
LED turns orange), and unplug the power cord.
Chapter 2 Observation Steps
(1) Focusing with the 40x objective by rotating the focus
(2) Focusing with the 10x objective by rotating the diopter
Switch to the objective to be used for observation.
Lights up in orange
49
knobs
rings
Adjust the diopter.
100x 60x 40x 20x
Adjust the aperture diaphragm.
OFF (Push)
Turn off the microscope.
10x 4x