Operation; Software; Quality Check Procedure; Setting Up The Quality Check - SYSMEX CyStain VitalCount Manual

4 Operation

4.1 Software

CyView™ is used for control of the device, data acquisition, data analysis and storage. For
more information, please refer to the CyFlow™ Cube 6 V2m manual.
FCS Express™ is used for reporting and analysis of data. The software can be accessed
directly from within CyView™. Refer to FCS Express™ manuals and tutorials for detailed
information about the software.
4.2

Quality Check procedure

4.2.1

Setting up the Quality Check

Requirements

Logged in to CyView™ with privileges of Main User level or above (Administrator)

LOT and FCS Express™ template match, see chapter 3.4 QC material LOT
dependency

CyStain™ VitalCount configuration is active

Priming has been performed

Sample tube with 850 μl Count Check Beads green, see chapter 3.3 Quality Check
material

Sample tube with 1500 μl Sheath Fluid
Procedure
1. Select [Work Mode].
2. Switch Measure Mode Sample Source to "Sample Port".
3. Connect the Sample tube with 850 µl Count Check Beads green.
4. Click [Start].
5. Adjust gain values in a way that the signals of the Count Check Beads green appear
exactly in the center of the quality gate "QC (CCB)" within the main Plot.
1
E.g. VitalCount_A_AK01_Robby.cvc85
CyStain™ VitalCount QC Procedure | QC Manual | June 2021
1 .
Figure 6:Selecting Work Mode
DQC Plot 1 DQC Plot 2
Figure 7:Adjust gain values
4 Operation
7