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Related Manuals for VWR XT96
Summary of Contents for VWR XT96
- Page 1 Instruction manual PCR Thermal Cycler XT / XTender ® EU cat. no US cat. no XT96 732-1359 76452-151 XT96 Gradient 732-3428 76452-153 XTender96 732-3657 76452-155 XTender96 Gradient 732-3658 76452-157 Version 2 Issue 17 October 2021...
- Page 2 VWR International Ltd Hunter Boulevard, Magna Park Lutterworth, Leicestershire, LE17 4XN uk.vwr.com United States VWR International LLC 100 Matsonford Rd Radnor, PA 19087 +1 800-932-5000 www.vwr.com Country of Origin : GERMANY vwr.com I Instruction manual VWR PCR Cycler XT ®...
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Page 3: Table Of Contents
Cleaning, maintenance and repair of the thermal cycler Returns only in original packaging Temperature check-up Calibration ORDERING INFORMATION TECHNICAL SERVICE WARRANTY COMPLIANCE WITH LOCAL LAWS AND REGULATIONS DISPOSAL INSTRUCTION LOCAL VWR OFFICES IN EUROPE AND ASIA PACIFIC vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 4: Introduction
INTRODUCTION The VWR thermal cyclers are the ideal PCR devices for all molecular biological and biochemical laboratories working in the field of basic research or routine diagnostics. The 250°C HTR (High Temperature Range) Peltier technology allows quick changes in temperature up to 4 °C/sec (max). The... -
Page 5: Licence Notice
Applera, including but not limited to U.S. Patent No. 6,814,934 and its non-U.S. counterparts, which describe and claim thermal cyclers capable or real- time detection. vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 6: Delivery Contents
1 Thermal cycler 1 Power cord 1 Instruction manual 1 Link PC-software VWR 'PCR Cycler XT Software' UNPACKING Remove the instrument from its packaging and inspect it for signs of damage. If any are discovered, inform your supplier immediately ... -
Page 7: Specifications
96 well Universal block with lid heating and automatic height adaption for 96 x 0.2 ml tubes • and 96 well PCR plates (non-skirted and half-skirted) Power supply 100 - 240 V AC, 50/60 Hz, max. 860 VA For indoor use, only! vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 8: Connections
CONNECTIONS Status LED 1x USB interface Main power switch 1x USB interface AC Power inlet Ethernet interface vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 9: Installation Of The Pcr Cycler Xtender 96
For safety reasons the device must be switched off and the mains plug pulled before cleaning is performed! INSTALLATION OF THE PCR CYCLER XTENDER The VWR thermal cycler XTender is a fully equipped PCR Thermal cycler without a Touch screen, the ideal device controlled by a PC or from another XT Thermal cycler. -
Page 10: Operating The Pcr Cycler
If the user has to enter numbers or text the corresponding field has to be touched on the touch screen or clicked with the mouse. An appropriate keyboard will appear on the screen according to the expected entry. vwr.com I Instruction manual VWR PCR Cycler XT ®... - Page 11 To enable a homogeny pressure of the heating lid, distribute single tubes and strips over the whole block. When using only one or a few tubes – load at least one empty tube in each corner of the block. Fig : Ideal positioning Tubes and Strips vwr.com I Instruction manual VWR PCR Cycler XT ®...
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Page 12: Main Menu
This dialogue is used for selecting, executing and monitoring existing PCR protocols. A detailed description of the 'Run' dialogue is given on page 14. Programs This dialogue is used for creating, editing and organizing PCR protocols (see page 20). vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 13: Change User
To change the settings or implement new users, please go to the User Management dialogue in the System dialogue (page 29) The factory-set password for the Administrator is admin. vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 14: Incubation Mode
Set temperatures of block and lid and time by entering the values of choice in the corresponding editor fields. By entering values < 35 °C / 104 ºF the lid heating will be turned off. vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 15: Run Dialogue
Run dialogue: Overview – “Simple Mode” The following functions are found in the 'Run' dialogue: Select Program Cycler selection User Program name Progressbar Time remaining Start Button Stop Button Expert Mode / Simple Mode vwr.com I Instruction manual VWR PCR Cycler XT ® Protocol Wizard... -
Page 16: Run Dialogue: Overview - "Expert Mode
The following functions are found in the 'Run' dialogue: Cycler selection Protocol window User Program name Select protocol Info Button Start Stop List view / Expert Mode / diagram view Simple Mode vwr.com I Instruction manual VWR PCR Cycler XT ®... - Page 17 PCR protocol can be selected and loaded. Also, the Protocol Wizard used create Protocols. If a recently used program is selected, the chosen program will be loaded in the run dialogue by clicking vwr.com I Instruction manual VWR PCR Cycler XT ®...
- Page 18 Using this button, a stored or recently used PCR protocol can be selected and loaded. Also, the Protocol Wizard can be used to create new Protocols. Info button Use this button to display details of a PCR protocol (name, program options, author, settings and comments). vwr.com I Instruction manual VWR PCR Cycler XT ®...
- Page 19 'Start' button. A program is running General: 'Pause' 'Stop' button After starting the chosen program, you can use these buttons to either pause or to stop the program. vwr.com I Instruction manual VWR PCR Cycler XT ®...
- Page 20 Remaining time Average temperature finished cycles is of PCR program of the thermal block shown Total Progress: Lid: Total Progress of Temperature of PCR program in % the heated lid vwr.com I Instruction manual VWR PCR Cycler XT ®...
- Page 21 GLP file will be saved as a default setting, to change this parameter go to ‘System/Thermal Block’ and deselect – ‘Always save GLP´s’ function. If the GLP Report is not needed press to deleted. vwr.com I Instruction manual VWR PCR Cycler XT ®...
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Page 22: Program Dialogue
Once a command is added the corresponding parameters will need to be adapted. To enter a parameter the desired input field has to be selected and click on or by double click. vwr.com I Instruction manual VWR PCR Cycler XT ®... - Page 23 This global ramp rate will be used in all temperature and gradient steps of the PCR program but could be changed in a single temperature or gradient step manually by entering the desired value at the step. vwr.com I Instruction manual VWR PCR Cycler XT ®...
- Page 24 The parameters of a command can be modified by selecting the corresponding command line of the program and changing it with the 'Edit' button. (The same function has a double click on the command line). vwr.com I Instruction manual VWR PCR Cycler XT ®...
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Page 25: Deleting A Command From The Program
List view Push this button to view the selected program in list view. Diagram view Push this button to view the selected program in diagram view. vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 26: Available Commands (Program Steps)
Incremental change of temperature with each successive cycle. The sign indicates whether the temperature will increase or decrease. Time Incr./Decr Incremental change of hold time with each successive cycle. The sign indicates whether the hold time will increase or decrease. vwr.com I Instruction manual VWR PCR Cycler XT ®... - Page 27 Use this button to define a program cycle (loop), consisting of several individual program steps. In the field 'Number of Cycles' enter the desired number of cycle repeats, (max. 99 cycles). vwr.com I Instruction manual VWR PCR Cycler XT ®...
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Page 28: Glps Dialogue
Displaying a GLP report After pushing the GLP button a dialogue appears to select a GLP report, which will then be shown. The dialogue can also be opened by pushing the button vwr.com I Instruction manual VWR PCR Cycler XT ®... - Page 29 . The GLP report is shown as displayed. Save Use this button to save a pdf file of the selected GLP report. Printer Use this button to print out the selected GLP report. vwr.com I Instruction manual VWR PCR Cycler XT ®...
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Page 30: Explorer Dialogue
Files and Folders Marked files and folders are shown with a blue background. Delete Use this button to delete marked Files and Folders Marked files and folders are shown with a blue background. vwr.com I Instruction manual VWR PCR Cycler XT ®... - Page 31 Start Pushing the button will start a selected PCR program directly from the Explorer mode. Only possible if no other PCR Program is running. vwr.com I Instruction manual VWR PCR Cycler XT ®...
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Page 32: Diagnose Dialogue
(un-selected zones are marked in gray) Navigation button: left / right Zoom-in and -out - Automatic scrolling Navigation button: Zoom-in and - horizontal up and down out vertical active or inactive vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 33: System Dialogue
The name of a user appears in the program header of a program generated by the user as well as in the GLP report of a program run by the user. vwr.com I Instruction manual VWR PCR Cycler XT ®... - Page 34 'Edit User'. Important: If a new user should only have limited rights, it might be useful to set up a new user group with the appropriate rights to access. vwr.com I Instruction manual VWR PCR Cycler XT ®...
- Page 35 Using this button, the time and date can be set. Remote Control This dialogue is used to connect a thermal cycler via network, to enable a remote control off the instrument. The following command buttons are available: vwr.com I Instruction manual VWR PCR Cycler XT ®...
- Page 36 Once a remote-control connection has been made via LAN the target cycler will be displayed in the LAN control window (in our example: XT96). To reconnect with this cycler, mark it in the overview and use the button 'Connect/Disconnect' to start a new connection.
- Page 37 Using this dialogue, the IP address for the network printer, can be entered. Please refer your network administrator for the correct printer path. Use the button produce a test print. vwr.com I Instruction manual VWR PCR Cycler XT ®...
- Page 38 Enable Gradient Enables the gradient function on a non- Gradient Thermal Cycler. Software key can be ordered separately (732-3482). Please ask your local VWR partner. Export Logs For troubleshooting, the thermal cycler record internal communication. The protocol files can be stored on a USB stick.
- Page 39 XTender Thermal Cycler. Note: Every Thermal Cycler needs a separate “XT96 Config File” where the instrument name is unique. Plug the “Network Configuration USB-Stick” into an USB port of the Thermal cycler or XTender vwr.com I Instruction manual VWR PCR Cycler XT ®...
- Page 40 Remove the “Network Configuration USB-Stick” from the USB port of the XTender power on the XTender thermal cycler Note: For each thermal cycler, a new “XT96 Config File” must be created. Licence Info Software licence of the XT GUI will be...
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Page 41: Maintenance
If there is a reasonable suspicion that the device has deviated from the calibration range, because the variations of the temperature check-up are out of the acceptable tolerance, a new calibration must be carried out by the manufacturer. Please contact your VWR representatives. vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 42: Ordering Information
(2) years from date of delivery. If a defect is present, VWR will, at its option and cost, repair, replace, or refund the purchase price of this product to the customer, provided it is returned during the warranty period. -
Page 43: Disposal Instruction
By doing so, you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health. Thank you vwr.com I Instruction manual VWR PCR Cycler XT ®... -
Page 44: Local Vwr Offices In Europe And Asia Pacific
LOCAL VWR OFFICES IN EUROPE AND ASIA PACIFIC Austria Hungary Poland VWR International GmbH VWR International Kft. VWR International Sp. z o.o. Graumanngasse 7 Simon László u. 4. Limbowa 5 1150 Vienna 4034 Debrecen 80-175 Gdansk Tel.: +43 1 97 002 0 Tel.: +36 52 521130...
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