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CALIBRATE VOLUME
DEFINE SAMPLE
CALIBRATE VOLUME:
↵
PRESS
WHEN READY!
TAKING ___ ml
CALIBRATE SAMPLE
VOLUME DELIVERED:
___ ml
___!
ARE YOU
SURE?
YES
4.6 Operate the Pump
Manually
↵
PRESS
TO
PUMP REVERSE
↵
PRESS
TO
PUMP FORWARD
Calibrating the sampler can enhance sample volume accuracy. To
calibrate sample volumes, follow the steps below. These steps
assume that the sampler has been correctly installed (section 2)
and the suction line has been cut and its length entered in the
sampler configuration (section 3.3.3). These steps also require a
graduated cylinder to measure the delivered sample volume.
Note
Teledyne Isco offers a 1000 ml graduated cylinder. Order part
number 299-0020-00.
To calibrate the sample volume:
1. At the Main Menu, press the Calibrate button.
2. Select the CALIBRATE VOLUME option and press Enter.
3. Pull the lower pump tube from the bulkhead fitting. Hold
the end of the tube over the graduated cylinder. Press the
Enter button when you are ready.
4. Hold the graduated cylinder while the sampler collects the
sample.
5. Measure the sample volume collected in the graduated cyl-
inder and enter it in the VOLUME DELIVERED screen.
6. If there is a large difference between the expected volume
and your entered volume, the sampler asks you to confirm
NO
the entered volume. If the volume you entered is correct,
select YES. Otherwise, select NO and re-enter the measure
sample volume.
7. Return the pump tube to the bulkhead fitting.
An alternative method using manually defined sample volumes
may be helpful for difficult applications. See section 3.5.10.
The sampler pump can be operated manually from the Main
Menu display.
To operate the pump:
1. Press the "1" Number button to prepare the pump for
reverse operation (line purge).
Or, press the "3" Number button to prepare the pump for
forward operation (sample).
2. Press the Enter button to start the pump in the selected
direction.
3. Press the Stop button to stop the pump and return to the
Main Menu.
Isco 4700 Refrigerated Sampler
Section 4 Operation
4-9
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