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Thermo Scientific Steri-Cycle i160 CR Operating Instructions Manual
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Thermo Scientific Steri-Cycle i160 CR Operating Instructions Manual

Co2 incubator
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Thermo Scientific
Steri-Cycle i160 CR/
Steri-Cycle i250 CR
CO
Incubator
2
Operating Instructions
50163206
March 2021

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Summary of Contents for Thermo Scientific Steri-Cycle i160 CR

  • Page 1 Thermo Scientific Steri-Cycle i160 CR/ Steri-Cycle i250 CR Incubator Operating Instructions 50163206 March 2021...
  • Page 2 Heracell Vios CR™, Steri-run™, Steri-cycle CR™, iCan™, THRIVE™ and Cell locker™ are registered trademarks of Thermo Scientific. Thermo Scientific is a brand of Thermo Fisher Scientific Inc. All other trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers.
  • Page 3 General Notes............................1 Identification of the Device and of the Documentation................1 Identification of the Device .......................1 Instruction of the Operating Personnel....................1 Applicability of the Instructions......................2 Original Document for Translation ....................2 Warranty ..............................2 Requirements for Warranty .......................2 Explanation of Safety Information and Symbols ..................3 Safety Information and Symbols used in the Operating Instructions ..........3 Additional Symbols for Safety Information: ..................4 Symbols on the Device ........................5...
  • Page 4 Installing the Support Stand with Castors ..................2-7 Support Stand with Castors and Supporting Feet (160i and 250i Unit) ......... 2-8 Installing the Floor Stand, 780 mm ..................... 2-10 Retrofitting/Modifications........................ 2-11 Steri-Cycle i160 / i250 CR Front View....................3-2 Steri-Cycle i160 / i250 CR Back View....................3-4 Safety Devices Steri-Cycle i160 / i250 CR ..................
  • Page 5 Mounting the Split Inserts (Optional) into the Steri-Cycle i250 CR ..........4-11 Gas Connection..........................4-12 Installing Gas Pressure Hoses ....................... 4-12 Gas Connection ........................... 4-13 Mains Connection ..........................4-14 Connecting the USB Interface: ......................4-15 Connecting the Alarm Contact: ......................4-15 Connecting the 4-20 mA Interface:....................
  • Page 6 Device Fusing Replacement......................9-11 Replacing the Outer Door Seal ......................9-11 Spare Parts............................9-12 Overview of the Materials Used: ....................... 10-1 Steri-Cycle i160 CR......................... 11-2 Steri-Cycle i160 CR......................... 11-4 Steri-Cycle i250 CR......................... 11-6 Steri-Cycle i250 CR......................... 11-8 USB Interface ..........................12-1 Installing the USB Port Driver ....................
  • Page 7 Example of a Code for a a Data Logger Query ................12-16 Program Steri-Cycle i160 / i250 CR ....................12-21 Installing the Steri-Cycle i160 CR & Steri-Cycle i250 CR ............12-22 Operating the Steri-Cycle i160 CR & Steri-Cycle i250 CR ............12-23 Installation of the Shelving .........................A-2...
  • Page 8 Steri-Cycle i160 / i250 CR...
  • Page 9 Figure 3-8. Interfaces (Right Side of Control Box) ................3-13 Figure 3-9. Steri-Cycle i160 / i250 CR with Glass Door ..............3-17 Figure 3-10. Steri-Cycle i160 CR with Threefold Gas-Tight Screen .............3-18 Figure 3-11. Water Reservoir ........................3-19 Figure 3-12. Fill and Drain Valve of Water Reservoir................3-19 Figure 3-13.
  • Page 10 Figure 4-10. Installation of Split Inserts ....................4-11 Figure 4-11. Installing Gas Pressure Hoses ....................4-12 Figure 4-12. Gas Connection ........................4-13 Figure 4-13. Mains Connection ......................4-14 Figure 4-14. Connection Example Alarm Contact ................4-16 Figure 4-15. Pin-out of the 4-20 mA Interface ..................4-17 Figure 5-1.
  • Page 11 Figure 6-31. Event Logging Menu......................6-28 Figure 6-32. Event Display........................6-28 Figure 6-33. Logging Cycle Setting ...................... 6-29 Figure 6-34. Displaying the Error Table....................6-31 Figure 6-35. Options Menu ......................... 6-32 Figure 6-36. Alarm Menu........................6-32 Figure 6-37. Alarm Relay Setting......................6-33 Figure 6-38.
  • Page 12 Steri-Cycle i160 / i250 CR...
  • Page 13 Identification data incubator Device name: Unit Name: Steri-Cycle i160 CR Steri-Cycle i250 CR Allocation of product documentation Documents CD-ROM 50163127 Certifications and quality audit: Certification: CE Certification Test mark: TÜV GS, cCSAus, EAC These operating instructions describe the CO incubator Steri-Cycle i160 / i250 CR. The CO incubator has been manufactured in keeping with the latest technological developments and is operationally safe.
  • Page 14 Should you encounter problems that are not detailed adequately in these operating instructions, please contact Thermo Scientific immediately for your own safety. Thermo Scientific warrants the operational safety and functions of the CO2 incubator for 2 years, only under the condition that: •...
  • Page 15 Indicates a hazardous situation which, if not avoided, could result in death or serious injuries. Indicates a hazardous situation which, if not avoided, could result in minor or moderate injuries. Indicates a situation which, if not avoided, could result in property damage. Is used for applicational hints and useful information.
  • Page 16 Wear safety gloves! Wear safety goggles! Harmful liquids! Electric shock! Hot surfaces! Fire hazard! Explosion hazard! Suffocation hazard! Tilting hazard! Steri-Cycle i160 / i250 CR...
  • Page 17 CE conformity mark: confirms conformity according to EU Guidelines TÜV test mark, 230 V devices TÜV test mark, 120 V devices, USA/Canada EAC conformity mark: confirms conformity according to all technical regulations of the Eurasian customs union (Russia, Kazakhstan and Belarus) Observe operating instructions! Hot surfaces! This CO...
  • Page 18 Typically, this CO incubator has been designed for being installed and operated in the following areas: • Laboratories for cytobiological and biotechnological experiments of safety levels L1, L2, and L3 • Medical-microbiological laboratories in accordance with DIN EN 12128 • Research labs in clinics and hospitals This device is intended for professional use only.
  • Page 19 Gases! No other gases than those specified in the intended use may be used. Installation work: Any work on supply lines and pressurized gas containers, cylinders or containers used for storing or O must only be carried out by expert personnel using the appropriate tools. It must be ensured that the exposure limits at work place for CO and/or O are not exceeded.
  • Page 20 is a gas that promotes combustion and may explode in combination with grease-containing materials. Oxygen explosion! Oxygen (O ) may explode in combination with oils, greases, and lubricants. If highly compressed oxygen comes in contact with grease- or oil-containing substances, the mixture may explode! Control panel and display window protective foil! •...
  • Page 21 • “Packaging” page 1-1 • “Examination on Receipt” page 1-1 • “Standard equipment Components Steri-Cycle i160 / i250 CR” page 1-2 • “Optional Equipment Components Steri-Cycle i160 / i250 CR” page 1-2 • “Optional Equipment Components Steri-Cycle i160 / i250 CR” page 1-3 The CO incubator...
  • Page 22 Steri-Cycle i160 / i250 CR Water reservoir cover panel Max. water level sensor Air duct, rear wall filter enclosure Air duct top Airbox with sealing package Prefilter Shelves Support rails for shelves Shelf support for inserts Plug for access port Exhaust filter for installation in rear wall filter enclosure - to be installed by the customer Power cord connecting hose set...
  • Page 23 Steri-Cycle i160 / i250 CR Threefold gas-tight screen Sixfold gas-tight screen Cell Locker Refill Funnel Steri-Cycle i160 / i250 CR Support brackets Split inserts Sixfold gas-tight screen Steri-Cycle i160 / i250 CR...
  • Page 24 Steri-Cycle i160 / i250 CR...
  • Page 25 • “Environmental Conditions” page 2-1 • “Site Ventilation” page 2-2 • “Space Requirements” page 2-2 • “Transport” page 2-3 • “Stacking” page 2-4 • “Retrofitting/Modifications” page 2-11 The device must only be operated at locations that meet the particular ambient conditions listed below: •...
  • Page 26 When CO is supplied, the work space of the incubator is slightly pressurized. The pressure is released through the pressure compensation opening into the operating room. Pressure compensation and any opening of the glass door/gas-tight screen during the operation of the device will release very small quantities of CO into the operating room.
  • Page 27 Lift the device only using the lift points. Heavy loads! Lift with care! The weights without accessories are for Steri-Cycle i160 CR - 95 kg and for Steri-Cycle i250 CR - 119 kg. To avoid injury through physical strain, such as strain traumata and slipped discs, do not attempt to lift the incubator alone! Use the required number of persons or lifting aids according to the weight.
  • Page 28 is suited for stacking up to two devices of the same device type. This is Steri-Cycle i160 / i250 CR accomplished by placing an optional stacking adapter (1/Figure 2-3) between the two units. As an option, a mobile base rack (2/Figure 2-3) is available in order for the units to be movable.
  • Page 29 1. Remove the 2 outer rear panel screws on both sides of the lower unit and store them. 2. Place the lower device on the mobile base rack on the fixing points so that the rear side rests against the connecting straps. 3.
  • Page 30 Condensate formation during the operation of stacked devices: As a rule when operating stacked devices of type Steri-Cycle i160 CR and Steri-Cycle i250 CR, an adapter plate should be placed between the two devices for thermal separation. If stacked devices are operated at an ambient temperature of more than 28 °C, an overtemperature alarm is triggered on the upper device while the Steri-run decontamination routine is run for the lower device.
  • Page 31 Possible stacking combinations Bottom stacking position Heracell Vios 160i CR Heracell Vios 250i CR Steri-Cycle i160 CR Steri-Cycle i250 CR 50148171 Heracell Vios 160i CR Steri-Cycle i160 CR 50154522 Heracell Vios 250i CR Steri-Cycle i250 CR Details see assembly instruction of stacking adapter kit.
  • Page 32 1 support stand, 4 screws M4x16 • Remove the 4 screws at the lower rear side of the unit. They will be replaced with the six new supplied screws. • Place the unit on the support stand, matching the nuts of the supporting feet. Make sure, that the holes of the removed screws are matching with the holes in the angles of the support stand.
  • Page 33 50161859 Support stand H = 76 mm with castors for Heracell Vios 160i CR and Steri-Cycle i160 CR 50162731 Support stand H = 76 mm with castors for Heracell Vios 250i CR and Steri-Cycle i250 CR...
  • Page 34 Side parts Spacer Hinged foot with check nut, height adjustable Fillister head screw M10 x 40, with distance tube Counter weight for 160 L units Open-end wrench SW 24 Allen key SW 8 • Screw the support profiles (20) onto the side parts (10) with a socket head screw / spacer tube (40). •...
  • Page 35 Vios CR and Steri-Cycle CR SW 24 The following components can be retrofitted to the standard version: Steri-Cycle i160 CR • threefold gas-tight screen (the gas-tight screen is installed in place of the glass door), • sixfold gas tight screen with or without Cell locker, •...
  • Page 36 Steri-Cycle i160 / i250 CR...
  • Page 37 • “Steri-Cycle i160 / i250 CR Front View” page 3-2 • “Steri-Cycle i160 / i250 CR Back View” page 3-4 • “Safety Devices Steri-Cycle i160 / i250 CR” page 3-4 • “Workspace Atmosphere” page 3-5 • “Door Switch” page 3-10 •...
  • Page 38 Steri-Cycle i160 / i250 CR Steri-Cycle i160 / i250 CR 1. Pressure compensation opening /Access port with plug 2. Door switch for glass door 3. Temperature Sensor 4. O sensor (optional) 5. Door handle for the glass door or the gas-tight screen 6.
  • Page 39 10. Water drain 11. Stand, height-adjustable 12. Glass door seal, replaceable 13. Airbox with seal and HEPA-filter 14. Shelves 15. Support rail 16. Insert with shelf support 17. - 18. IR CO sensor 19. Door handle, outer door 20. iCan™ Touchscreen (Control panel) 21.
  • Page 40 Steri-Cycle i160 / i250 CR Steri-Cycle i160 / i250 CR 1. Control box with supply interface for combined gas connection (optional) CO and O 2. Diagram: CO and O gas connection without gas monitoring 3. O gas cylinder 4. CO gas cylinder Steri-Cycle i160 / i250 CR The device is equipped with the following safety devices:...
  • Page 41 For continuous operation, a sufficient quantity of processed water with the recommended water quality must be provided: • Max. fill volume for Steri-Cycle i160 CR and Steri-Cycle i250 CR: 3 l. For trouble-free operation, fill the water reservoir with sterilized distilled water or water treated to an equivalent quality level.
  • Page 42 No tap or ultrapure water in humidity reservoir Sterile distilled water or water treated to an equivalent quality level is the recommendation for use in the integrated humidity reservoir. The acceptable conductivity should be within the range of 1 to 20 µS/cm (resistivity should be within the range of 50 K-Ohm-cm to 1 M-Ohm-cm).
  • Page 43 The air stream from the water reservoir towards the workspace is channeled through a HEPA-filter in order to minimize the risk of contamination. The filter works with separation rate of 99.998 % for a particle size of 0.3 µm (HEPA-filter quality). The HEPA-Filter (2/Figure 3-3) must be inserted into the airbox...
  • Page 44 The air duct consists of two shaped pieces of sheet metal: 1. Ceiling duct (1/Figure 3-4) 2. Rear wall duct (2/Figure 3-4) 3. Back wall of the workspace (3/Figure 3-4) The air duct and HEPA-filter can be installed and removed without any tooling. To ensure the growth conditions for the cell and tissue cultures, the workspace is supplied with CO The pH of the bicarbonate-buffered culture media largely depends on the CO content of the...
  • Page 45 If the oxygen content during operation is to be lowered to less than 21% (air oxygen content), the workspace is supplied with nitrogen. The O concentration in the workspace atmosphere can be controlled depending on sensor technology. A HEPA filter with 12 hexagonal bolts must be installed in the rear wall filter enclosure before the device is commissioned.
  • Page 46 A door switch is installed at the upper edge of the workspace opening. When the door switch is activated by opening the glass door, the gas supply and workspace heating are suspended. The display shows a corresponding message. An alarm sounds, if the door is open longer than 30 seconds. If the door remains open for more than 10 minutes, an audible alarm sounds and the alarm relay responds.
  • Page 47 • O sensor (optional) for the acquisition of the oxygen content in the workspace atmosphere (2/Figure 3-6). • CO sensor for the acquisition of the CO content in the workspace atmosphere (3/Figure 3-6). • rH sensor for monitoring the humidity of the workspace atmosphere (4/Figure 3-6).
  • Page 48 The sensor for the acquisition of the workspace temperature as well as the CO sensor and the optional sensor are integral to the control system of the device. Their measured values are compared to the selected set values. Based on this data, the control system controls heating and CO supply.
  • Page 49 Steri-Cycle i160 / i250 CR...
  • Page 50 16 bit per channel, equivalent to 65536 incremental values. The interface port accepts various customer-supplied external measurement devices with 4-20 mA inputs - for example, the Thermo Scientific Smart-Vue Wireless Monitoring System (not supplied). Steri-Cycle i160 / i250 CR...
  • Page 51 The table below lists the output signals: Temperature 0.08 MA/°C 0...200 °C 4...20 mA concentration, 0.8 mA/% 0...20% CO 4...20 mA - concentration* 0.16 mA/% 0...100% O 4...20 mA Not used Parameters marked with an asterisk “*” are only output if the respective option is installed. The measured samples are governed by the following function: –...
  • Page 52 The device can be connected to an on-site, external alarm system (such as a private branch telephone exchange, a facility monitoring system, visual or audible alarm indicators. For this purpose, a potential-free alarm contact is preinstalled in the device. This contact is broken out to the control box on the backside of the unit (5/Figure 3-8).
  • Page 53 The standard version of the Steri-Cycle i160 CR and Steri-Cycle i250 CR units is equipped with a single-locked door made of tempered safety glass.
  • Page 54 Steri-Cycle i160 CR Each door of the gas-tight screen (2/Figure 3-10) is separately locked (1/Figure 3-10). The water reservoir is sunk into the interior container bottom and separated from the workspace by means of a cover panel. A drain (2/Figure...
  • Page 55 The water reservoir is monitored by the water level sensor described in section “Sensor System” page 3-10. A fill level indicator with the punched-out word “MAX“ (1/ Figure 3-11) is suspended above the water reservoir to indicate the maximum fill level. The maximum fill volume of the water reservoir is 3 liters. In order to minimize interference to the workspace atmosphere during water replacement without disruption of incubation operation, the unit has a front-side quick-drain valve.
  • Page 56 Through the water valve at the incubator, water can be filled with a re-filling funnel. Hang-in the hooks of the refill device in the locks of the glass doors as shown above. Connect the hose to the fill and drain valve. The refill funnel determines the height difference of the water level in the incubator, according to the principle of the communicating tubes.
  • Page 57 The access port with insert (Figure 3-15 Ø 42 mm / 1.66 inch) can be sealed with a plug (1/Figure 3-15) and allows for feeding cables, tubes or additional sensors into the workspace of the unit. The pressure compensation opening (3/Figure 3-15) located directly beneath the access port in the rear wall of the unit ensures pressure compensation between the workspace of the unit and the operating room.
  • Page 58 3-16) have an integrated tilt protection and withdrawal stop. Steri-Cycle i250 CR models come equipped with a single-piece bottom insert, as shown on the left side in Figure 3-16, Steri-Cycle i160 CR with a cutout for refilling (4/Figure 3-16). For details on using the shelf system, “Start-up”...
  • Page 59 The electromechanical door lock consists of a rotary latch (detail A in Figure 3-17) driven by a built-in motor, a built-in mechanical emergency release lever (detail C) and a closing hook (detail B) mounted on the inner side of the door. The door lock is a safety device that denies access to the incubator‘s workspace while the Steri-run decontamination routine is running.
  • Page 60 Steri-Cycle i160 / i250 CR...
  • Page 61 • “Adapting the Unit to the Ambient Atmosphere” page 4-1 • “Preparing the Workspace” page 4-1 • “Installing the “MAX” Fill Level Indicator and the Prefilter” page 4-2 • “Install the Air Duct” page 4-4 • “Installing the Shelf System” page 4-9 •...
  • Page 62 • Prefilter • Air duct • Airbox • Shelves • Workspace surfaces • Glass door seal • Glass door/gas-tight screen. Decontamination: For details about the cleaning and disinfection of the device see chapter “Cleaning and Disinfection” page 8-1. The “MAX” fill level indicator and the prefilter can be installed without any need for tools: 1.
  • Page 63 5. Insert the prefilter (4/Figure 4-2) into the water reservoir cover panel. Steri-Cycle i160 / i250 CR...
  • Page 64 A HEPA filter with 12 hexagonal bolts must be installed in the rear wall filter enclosure before the device is commissioned. Back View Front View HEPA filter holder Exhaust filter The HEPA filter is placed on the holder and fixed with the 12 hexagonal bolts. Do not touch the surface of the HEPA filter.
  • Page 65 Steri-Cycle i160 / i250 CR...
  • Page 66 4 – sealing parts Steri-Cycle i160 CR Do not remove the air duct seals on the Steri-Cycle i160 CR versions. The rear wall air ducts are important for a proper air flow on VIOS160 models with a stainless steel inner chamber.
  • Page 67 CLICK 1. Place the HEPA-filter (2/Figure 4-5) on a flat surface. 2. Tilt the airbox (1/Figure 4-5) to the left and insert the tabs on the left (3/Figure 4-5) into the corresponding slots provided in the HEPA-filter (4/Figure 4-5). 3. Insert the tabs (5/Figure 4-5) on the right side of the airbox into the HEPA-filter catches (6/Figure...
  • Page 68 5. Place the airbox (1/Figure 4-6) onto its seat (2/Figure 4-6) on the water reservoir cover panel. 6. Mount the water reservoir cover panel on the bottom of the workspace. 7. Lift the water reservoir cover at the front side and push it towards the rear wall (1/Figure 4-7).
  • Page 69 The HEPA-filter (2/Figure 4-5) must be inserted into the airbox (1/Figure 4-5) from below. The airbox rests on the water reservoir cover panel (2/Figure 4-6) and is pushed against the air intake. Tools are not required for the installation of the shelf system. The support rails are secured using spring pressure.
  • Page 70 2. Clamp the lockspring behind the upper embossing. 3. To remove the support rails, pull the lockspring tab down out of the embossing and remove the rail. 1. Insert the shelf supports into the perforations of the support rail (1/Figure 4-9) with the bar (2/Figure 4-9) facing down.
  • Page 71 3. Make sure that the shelf support is positioned in the two tilt protections in a way that allows it to move freely. With the Steri-Cycle i160 CR incubator be sure to install the insert with the cut-out for refilling at the bottom position only. For sixfold gas tight screen the shelf supports are installed in the rectangular holes only, installing the shelves, see Appendix.
  • Page 72 Gas quality: The gases must have one of the following qualities: • Purity 99.5 % min • medical gas quality. Overpressure! The operating pressure of the gas applied to the device must not exceed 1 bar (14.5 psi). If the gas is supplied at a higher pressure, the valves integral to the device may not close correctly and the gas supply control may be impaired.
  • Page 73 Pressure compensation opening To ensure permanent pressure compensation, the pressure compensation opening must not be connected to an exhaust air system. The pipe of the pressure compensation opening must not be extended or redirected. The gas supply from the gas supply system to the device is established using the supplied flexible gas pressure hoses: Steri-Cycle i160 / i250 CR...
  • Page 74 Electric shock! Contact with current-carrying components may cause a lethal electric shock. Before connecting the device to the power supply, check plug and power supply cable for damage. Do not use damaged components when connecting the device to the power supply! Connect the unit to a correctly installed and grounded power supply source fused with a B 16 circuit breaker.
  • Page 75 6-23. Skilled work: Thermo Scientific warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately...
  • Page 76 Error power failure “ON” Legend: X: Contact closed / O: Contact open Switching structure: The alarm relay switches for all failures reported by the control loops (see “Error Messages” page 6-44). The connector for connecting the interface cable is not included in the scope of delivery and must be ordered separately.
  • Page 77 The following illustration shows the pin-out of the 4-20mA data interface: Skilled work: Thermo Scientific warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately...
  • Page 78 Connecting the 4-20 mA Interface: Steri-Cycle i160 / i250 CR...
  • Page 79 • “Preparing the Device” page 5-1 • “Starting Operation” page 5-2 The device must not be released for operation before all major start-up activities (see “Start-up” page 4-1) have been completed. Prior to starting operation, the following device components must be checked for their correct function •...
  • Page 80 When the filling level drops below a minimum value, it must be possible to top up water during the work process. Filling charge Steri-Cycle i160 CR and Steri-Cycle i250 CR: 3 l 1. Open glass door or gas-tight screen. 2. Remove prefilter (2/Figure 5-1).
  • Page 81 Through the water valve at the incubator, water can be filled with a re-filling funnel. Hang-in the hooks of the refill device in the locks of the glass doors as shown above. Connect the hose to the fill and drain valve. The refill funnel determines the height difference of the water level in the incubator, according to the principle of the communicating tubes.
  • Page 82 5. Do not exceed the maximum level mark “MAX” (1/Figure 5-4). The maximum fill volume of the water reservoir (5/Figure 5-4) is 3 l. 6. Wipe off excess water from the cover of the water reservoir. 7. Reinstall prefilter (2/Figure 5-1).
  • Page 83 18. Load the workspace with cultures. Duration of the auto-start routine: When the device is cold and when the ambient temperature is low, the auto-start routine may take up to 10 hours. Loading: To ensure sufficient air circulation and even heating of the samples, the loading surface within the workspace should be used up to 70% max.
  • Page 84 Steri-Cycle i160 / i250 CR...
  • Page 85 • “Power Switch” page 6-2 • “Operating Panel and Operating Structure” page 6-3 • “Factory Presettings of the iCan™ Touchscreen Controls” page 6-7 • “Heat-up Phase of the Control Loop Sensors” page 6-7 • “Behavior of Keys during Adjustments” page 6-8 •...
  • Page 86 The power switch is mounted in the side panel of the unit. • Powering up: Press the power switch; the switch illumination comes on. • After a short beep the blank screen vanishes and the touchscreen is displayed. • The control loop sensors pass the heat-up phase (“Heat-up Phase of the Control Loop Sensors”...
  • Page 87 The control panel works as a touchscreen (iCan™ Touchscreen) and can be controlled performing light pressure using a finger or a blunt pin. The following screen areas of the operating panel are touch-sensitive and accept operating commands: • Temperature display T, •...
  • Page 88 Function keys and actual value readouts on the operating panel for versions without O gas supply source: 1. Workspace temperature display field with actual value (large numeric readout at center), set value (small numeric readout top right) and physical unit (bottom right) 2.
  • Page 89 Function keys and actual value readouts on the operating panel for versions with a combined gas supply source: 1. Workspace temperature display field with actual value (large numeric readout at center), set value (small numeric readout top right) and unit (bottom right) 2.
  • Page 90 Steri-Cycle i160 / i250 CR...
  • Page 91 Upon delivery of the device, the following set values have been preset: • Temperature: 37 °C • CO -content: 5.0 % • O -content (optional): 21.0 % control: Since the CO concentration of the air is nearly 0%, the CO control and the control loop error monitoring system are disabled at a set value of 0%.
  • Page 92 Pressing a key can increase or reduce a value gradually: • Keeping the “–“ or “+” key depressed switches to rapid scan mode, • after the key has been kept depressed for more than 3 seconds, the quick run speed increases. Saving the settings: To save changed values, press the Enter key.
  • Page 93 To increase the set value: • Press the + key. To reduce the set value: • Press the - key. 4. To accept and save the set value: • Press the Enter key. • The system returns to the main menu. The temperature displays shows the actual value currently measured in the workspace.
  • Page 94 3. To set the CO set value: • Press the Set value key. To increase the set value: • Press the + key. To reduce the set value: • Press the - key. 4. To accept and save the set value: •...
  • Page 95 2. To exit the O menu: • Press the End key. 3. To set the O set value: • Press the Set value key. To increase the set value: • Press the + key. To reduce the set value: • Press the - key. 4.
  • Page 96 Ventilate the inner chamber after changing the O setpoint so that no alarm occurs. Factory settings: Depending on the type of the O sensor, one of the two O control ranges has been preset at the factory: Control range I: 1 % to 21 % Control range II: 5 % to 90 % Usage of the process gases: For set values below 21 % O...
  • Page 97 Notes on using the auto-start routine: To ensure that the specified accuracy of the CO measuring system is maintained, the device should always be started using the auto-start routine if: • a difference of more than 1 °C is entered upon setting the temperature set value, •...
  • Page 98 Auto-start routine aborted due to error: The auto-start routine is aborted if: • an error is detected in the temperature control loop, • an error is sensed in the CO control circuit, • the water filling level is insufficient, • the CO value to be set is out of tolerance.
  • Page 99 6. Close both device doors. • A status display window showing data on the process is displayed. Cancellation: The auto-start routine can be canceled at any time! Press the Stop key. Automatic restart: The auto-start routine is restarted automatically if the routine is canceled due to one of the following events: •...
  • Page 100 3. To accept the failure message: • Press any position on the display. • The Error dialog box with the description of the error is displayed. 4. To accept the failure message: • Press the End key. • The system returns to the main menu. The Steri-run decontamination routine is used to decontaminate the complete workspace.
  • Page 101 The user configuration settings allow the user interface and the additional device functions to be adjusted to the requirements of everyday operation.The user configuration selection menu (Figure 6-17) is invoked by pressing the Menu key on the main screen. The user configuration menu is split into six categories: •...
  • Page 102 The keypad lock prevents the unauthorized changing of the operational settings. Only those keys are locked where values can be entered. The keypad is locked by entering four numbers. • Upon delivery, the default code is: 0000. This default can be changed into a user-defined code that is then enabled using the KEYPAD LOCK dialog box (“Enabling/Disabling the Keypad Lock”...
  • Page 103 4. Confirm your input: • Press the Enter key. • The Code new prompt appears in the display. Four blanks indicate that a new key code may be entered. Entering the new 4-digit code: • Press the corresponding numeric keys. •...
  • Page 104 3. For changing the date, choose the Date option. • The input dialog shown in Figure 6-22 is displayed. 4. To enter the date: • Press the numeric keys. • The input numbers are displayed in the input box. 5. To set the cursor to the left to overwrite a value: •...
  • Page 105 9. To enter the time: • Press the numeric keys. • The input numbers are displayed in the input box. 10. To set the cursor to the left to overwrite a value: • Press the Backspace (<<) key. 11. To accept and save the input value: •...
  • Page 106 4. To increase the value: • Press the + key. 5. To reduce the value: • Press the - key. 6. The value change appears in the display. The New message indicates that the selection has been changed, but not saved yet. 7.
  • Page 107 1. To increase the value: • Press the + key. 2. To reduce the value: • Press the - key. 3. The value change appears in the display. The New message indicates that the selection has been changed, but not saved yet. 4.
  • Page 108 3. Select the option Interface USB (Figure 6-26). • The input dialog shown in Figure 6-27 is displayed. 4. Press the + or key to set the Baud rate of the USB interface (Figure 6-27). • To increase the baud rate: Press the + key. •...
  • Page 109 The input dialog allows the language of the display screen to be set. Seven languages are available: • German, • English, • Spanish, • French, • Italian, • Chinese, • Japanese. Selecting the display language: 1. Press the Menu key. 2.
  • Page 110 The reminder intervals are integral components of the alarm and monitoring system of the device control. For the two essential functions Steri-run and auto-start as well as for routine service work, the user can set dates that trigger an alarm whenever they occur. The counting begins at 00:00 hrs of the day on which the previously set reminder interval has elapsed.
  • Page 111 1. To increase the number of days: • Press the + key. 2. To reduce the number of days: • Press the - key. 3. The value change appears in the display. The New message indicates that the selection has been changed, but not saved yet.
  • Page 112 The event display uses short, single-line entries specifying date and time to report the events that were logged during the operation of the device. The entries are listed in chronological order with the most recent event at the top position. The list can be displayed but not edited.
  • Page 113 1. To browse forward in the list: • Press the Continue key. 2. To browse backward in the list: • Press the Previous key. 3. Exit the display: • Press the End key. • The system returns to the Event logging menu. Due to limited memory resources, the oldest entries are deleted as new entries are logged.
  • Page 114 10 s 22.5 hours 30 s 2.8 days 60 s 5.6 days 120 s 11.2 days 180 s 16.8 days 3600 s 336 days The setting controls the logging cycle in sections of seconds during which the control loop measured values are logged during the operation of the device and displayed by the trend display (“Scaling the Trend Display”...
  • Page 115 Troubleshooting: For a detailed overview of causes for errors and their correction, please refer to the end of this chapter! 1. To browse forward in the error table: • Press the Continue key. 2. To browse backward in the list: •...
  • Page 116 The alarm relay is the electrical interface for wiring the internal control subsystem of the unit to an external power monitoring system. Depending on the required input signal of the external monitoring system, network monitoring can be enabled or disabled. If network monitoring is enabled, a power failure is detected as an error.
  • Page 117 1. To toggle between two states: • Press the + key. • Press the - key. 2. To accept and save the change: • Press the Enter key. • The system returns to the Options menu. If condensation occurs on the culture containers due to high relative humidity, the humidity in the workspace can be set to a lower level.
  • Page 118 1. To toggle between two states: • Press the + key. • Press the - key. 2. To accept and save the change: • Press the Enter key. The system returns to the Options menu. Upon return to the main menu the Low humidity icon appears. Low humidity: The enabling/disabling of the Low humidity function is entered into the event list.
  • Page 119 3. Select the menu command Gas tight screen. • The input dialog shown in Figure 6-39 is displayed. 1. To toggle between two options: • Press the + key. • Press the - key. 2. To accept and save the change: •...
  • Page 120 1. To toggle between two states: • Press the + key. • Press the - key. 2. To accept and save the change: • Press the Enter key. • The system returns to the Options menu. If the device-integral monitoring system detects an error: •...
  • Page 121 1. To toggle between two states: • Press the + key. • Press the - key. 2. To accept and save the change: • Press the Enter key. • The system returns to the Options menu. Depending on the requirements to the work process, the O control can be switched on and off.
  • Page 122 1. To toggle between two states of the O control: • Press the + key. • Press the - key. 2. The value change appears in the display. The New message indicates that the selection has been changed, but not saved yet. 3.
  • Page 123 If the unit is to be operated without the built-in HEPA-filter, the latter must be de-activated in the configuration menu to avoid malfunctions. 1. Press the Menu key. 2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed.
  • Page 124 2. To accept and save the change: • Press the Enter key. • The system returns to the Options menu. • After 5 minutes the activity indicator ISO 5 for the HEPA filter appears in the icon bar on the main menu (Figure 6-2 page...
  • Page 125 This function indicator shows that the keypad lock has been activated so that currently the settings cannot be changed (see “Enabling/Disabling the Keypad Lock” page 6-41). This function indicator shows that the relative humidity in the workspace has been lowered from approx.
  • Page 126 This menu shows the unit software versions in the display. • Exit the display: • Press the End key. • The system returns to the User configuration menu. The trend display of the three control loops: • temperature, • 0...20% CO •...
  • Page 127 • The CO menu (Figure 6-9) is displayed. 2. Press the Show trend icon. • The progress indicator appears. 3. To display an enlarged section: • Open a rectangular section in the desired area of the diagram using a finger or a pen. The size of the rectangle is determined by dragging a diagonal from the start (press onto screen at left of upper diagram frame) to the end (release at right of lower diagram frame.
  • Page 128 4. To return to the total trend: • Open rectangle over a small diagram section and press any position outside the marked area. 5. To exit the trend display: • Press the End key. • The system returns to the main menu. Logging cycle: The time interval of the data logging cycle can be redefined in the Recording interval dialog box (“Changing the Logging Cycle Time:”...
  • Page 129 When the controller of the unit has activated overtemperature protection and switched over to emergency control mode, the main menu is overlaid by a flashing warning triangle and the pertinent icon. 1. Displaying the cause of an error: • Press anywhere on the touchscreen. •...
  • Page 130 2. To exit the error display: • Press the End key. • The error message disappears. • The temperature display field is displayed with a red bounding box. 3. Resetting the error message: • Turn the device off. 4. Open the door and allow the workspace to cool down. 5.
  • Page 131 The error tables indicate source of error, cause of error, and possible corrections. For any communication with the Technical Service of Thermo Fisher Scientific, please have the device data ready. Steri-Cycle i160 / i250 CR...
  • Page 132 System Device door open too Doors open for more Close device doors long than 10 minutes Failure: Display Display not Reset unit. If the communicating with error occurs the main board repeatedly, call service. Failure: EEPROM EEPROM on main Reset unit. If the main board board is defective error occurs...
  • Page 133 Tempera- Sensor breakage Measured value exceeds Request service. ture accepted limit Dry the heaters. Actual value above Actual value > Set value Do not exceed per- *2) *4) + 1 °C missible ambient temperature / call field service. Actual value low Actual value >...
  • Page 134 0...20% Sensor breakage Measured value exceeds Run auto-start. accepted limit When the error occurs again, trou- bleshoot the unit on the basis of section “Measures After Power Outage” page 6-46. When the error persists, request service. Actual value above Actual value > Set value automatic + 1% Actual value below...
  • Page 135 0...100% Sensor breakage Measured value exceeds Request service accepted limit Actual value above Actual value > Set value Check the gas supply + 1% source. Reduce primary pressure to 1 bar (14.5 psi) max. Actual value below Actual value < Set value Check the gas supply - 1% source.
  • Page 136 When this error occurs, a special control mode is activated to protect the samples. An icon appears to indicate this mode. waiting time before error message: - 45 min. after a door was opened, - 159 min. after a setpoint change. This setting can be changed by field service.
  • Page 137 • “Shutting the Device Down” page 7-1 Contamination hazard! The surfaces of the work space may be contaminated. If the useable space surfaces are contaminated, germs may spread to the environment of the incubator. In case of a shut-down, the device must be decontaminated! 1.
  • Page 138 8. Turn the device off using the power switch when the Steri-run decontamination routine has been completed. 9. Unplug the power connector and protect it against accidental reconnection. 10. Close shut-off valves of the supply system. 11. Disconnect the gas pressure hoses from the sleeve at the rear of the device. 12.
  • Page 139 • “Cleaning” page 8-1 • “Decontamination Procedures” page 8-2 • “Preparing for Disinfection / Steri-run” page 8-2 • “Wipe/Spray Disinfection of the Workspace” page 8-3 • “Steri-run Decontamination Routine” page 8-7 Incompatible cleaners! Some device components are made of plastic. Solvents can dissolve plastics. Strong acids or caustic solutions can cause to become brittle of the plastic.
  • Page 140 For Cell locker cleaning and disinfection see separate Cell locker operating instructions in the Appendix). The operator must prepare hygiene regulations for the decontamination of the device in accordance with the application of the device. The following disinfection procedures are compatible with the device: The wipe/spray disinfection is used as the standardized manual disinfection procedure for the device and for all accessories.
  • Page 141 The manual wipe/spray disinfection is carried out in three stages: • Predisinfection • Cleaning • Final disinfection • Alcoholic disinfectants! Disinfectants having an alcohol content of more than 10% may form, in combination with air, easily combustible and explosive gas mixtures. When using such disinfectants, avoid open flames or exposure to excessive heat during the entire disinfection process! •...
  • Page 142 Health hazard! The surfaces of the workspace may be contaminated. Contact with contaminated cleaning liquids may cause infections. Disinfectants may contain harmful substances. When cleaning and disinfecting, always observe the safety instructions and hygiene regulations! • Wear safety gloves. • Wear safety goggles. •...
  • Page 143 CLICK 3. Pull top part the air duct (1/Figure 8-3) towards the front side of the unit, then lift out with a downward movement when the keyholes in the front-side mounting tabs clear the retaining pins in the workspace ceiling. 4.
  • Page 144 5. Unhinge the rear part of the air duct (2/Figure 8-3) from the rear wall and remove it. 6. Remove prefilter. The prefilter is autoclavable. 7. Remove the cover panel from the water reservoir. 1. Thoroughly remove dirt residues and deposits using a solution of tepid water and dishwashing agent. 2.
  • Page 145 90 minutes. The effectiveness of the Steri-run decontamination routine has been certified by independent institutes. The germ reduction efficiency achieved is equal to 10 (6-log reduction) to the ISO 11138 standard. On request, Thermo Scientific will supply information on the pertinent tests.
  • Page 146 1. Before starting the decontamination routine, remove the silicone plug from the access port in the workspace. The silicon plug on the rear must be fitted. 2. Before starting the 180°C disinfection routine, one (of two possible) gas inlet filters should be screwed into the pressure compensation opening.
  • Page 147 The remaining run time of the Steri-run decontamination routine describes the time between the start or the current time status to the end of the cool-down phase. The indicated remaining run times are not measured values but merely used for orientation. The routine is divided into three phases: 1.
  • Page 148 The Steri-run decontamination routine is used to decontaminate the complete workspace. 1. Press the Steri-run key. To avoid damage to the silicone, sure to remove the silicone plug from the inner chamber before starting the Steri-run decontamination routine. • The Steri-run instruction menu is displayed. 2.
  • Page 149 • remaining run time. The Steri-run decontamination routine can be interrupted at any time. 1. Cancel Steri-run: • Press the Stop key. • If the Stop key in the status display is depressed, the Steri-run stop dialog box is displayed for a safety scan.
  • Page 150 If an error occurs while the decontamination routine is run, an error message is displayed and the following actions are initiated: • the decontamination routine automatically changes to the cool-down phase, • the audible alarm sounds. 1. To confirm the audible alarm: •...
  • Page 151 When all three phases have been completed, the Steri-run End (Figure 8-8) dialog window is displayed automatically. The decontamination routine must be stopped manually. • To stop Steri-run: • Press the End key. • The system returns to the main menu. Opening door during Steri-run decontamination: If the door will be opened and closed again whilst Steri-run decontamination is running, the routine returns to a phase which will ensure a continuation without faults.
  • Page 152 The door cannot be opened immediately after the Steri-run decontamination routine has been aborted by the user or due to an error. To release the outer door lock before the temperature has dropped to a non-hazardous level of 65°C, you must actuate the emergency release lever (item 2 in Figure 8-9): •...
  • Page 153 • “Inspections and Checks” page 9-1 • “Service Intervals” page 9-2 • “Preparing Temperature Calibration” page 9-2 • “Temperature Calibration Procedure” page 9-3 • “Preparing the CO2 Calibration” page 9-5 • “CO2 Calibration Procedure” page 9-6 • “Replacing the HEPA Filter in the Workspace” page 9-7 •...
  • Page 154 • Perform Technical Service service inspection. Service contract: Thermo Scientific offer a device-specific service contract that comprises all test and service works required. To determine the exact measured value of the incubator’s integral temperature sensor, a temperature comparison measurement must be performed every three months. If a major temperature deviation is found during this check, temperature calibration is required.
  • Page 155 1. Turn on the device using the power switch. set value and allow the device to heat up. This may take several hours. Set the temperature 3. Place the measuring instrument (2) onto the center area of the workspace. Alternatively, a temperature sensor may be positioned in this location. Route the connecting cable either through the measurement opening in the glass door (3) or through the access port (1) in the rear panel of the device.
  • Page 156 2. To exit the Temperature menu: • Press the End key. 3. To enter the Calibration submenu: • Press the Calibration key. 4. Enter the measured value (target value): The targeting value can be increased or reduced in increments; if you keep the + key or the – key depressed, the function switches to a rapid increase/reduction;...
  • Page 157 • The system returns to the main menu. The temperature displays shows the actual value currently measured in the workspace. Excessive workspace temperature: Excessive workspace temperatures after the calibration can be reduced by leaving the doors open for approx. 30 seconds. Value reset: If the value isn’t changed within the next 30 seconds, the system automatically exits the menu, and the most recently confirmed value is preserved.
  • Page 158 1. Turn on the device using the power switch. 2. Adjust the CO concentration to 4% and wait until the unit has fully heated up and developed the correct humidity level. 3. Insert the measuring instrument probe through the measurement opening into the workspace. Wait until the CO value displayed by the instrument has stabilized.
  • Page 159 4. Enter the measured value (target value): The targeting value can be increased or reduced in increments; if you keep the + key or the – key depressed, the function switches to a rapid increase/reduction; after approx. 3 seconds, another increase/reduction occurs.
  • Page 160 3. Lift the water reservoir cover at the front side (1/Figure 9-6). 4. Pull the water reservoir cover out at the front (2/Figure 9-6). 5. Remove the airbox with the HEPA filter. 6. Turn the airbox upside down, unlatch the tabs (5/Figure 9-7)on the left-hand side from the HEPA filter tabs...
  • Page 161 CLICK 8. Install the new HEPA-filter in airbox (1/Figure 9-7) and engage the locking tabs. 9. Place the airbox on its seat in the water reservoir cover panel. 10. If the unit has been operated without the HEPA-filter previously, activate the HEPA-filter in the user configuration according to instructions “Activating / De-Activating the HEPA-Filter:”...
  • Page 162 The HEPA filter in the rear wall filter enclosure is fixed with 12 hexagonal bolts. Loosen the 12 hexagonal bolts and store them in a safe place. Take the new HEPA filter out of the packaging and make sure that the inner filter surface is not touched. Insert the new filter and screw in the 12 hexagonal bolts crosswise and then tighten them.
  • Page 163 4. Unscrew the gas inlet filter(1/Figure 9-8) from the threaded hole (2/Figure 9-8). 5. When installing the new gas inlet filter, make sure that the plastic thread is not canted. Install the filter using caution. Procedure for gas supply gas inlet filter: 6.
  • Page 164 50164468 Magnetic door seal 160L 50164469 Magnetic door seal 250L 50164488 Set of filter mats for supply air 50162778 HEPA filter for exhaust air 50145367 Sintered metal filter 50141920 Airbox HEPA filter 50148315 Airbox seal 50142223 Silicone plug 50050737 Gas inlet filter 50145362 PM Kit Vios 50034772...
  • Page 165 • “Overview of the Materials Used:” on page 10-1 Contamination hazard! The device may have been used for treating and processing infectious substances. Therefore, the device and device components may have been contaminated. Prior to disposal, all device components must be decontaminated! •...
  • Page 166 Overview of the Materials Used: Outer door Stainless steel 1.4307, brushed. Door inner panel 1.4301 stainless steel sheeting Control panel and display window protective foil Polyethylene Heater Silicone-sheathed resistance heater wires Workspace containers, installed components and Stainless steel 1.4301, shelves Copper Insert for pressure compensation opening Stainless steel 1.4301 (base), 1.4404 (sinter filter)
  • Page 167 • “Steri-Cycle i160 CR” page 11-2 • “Steri-Cycle i250 CR” page 11-6 Steri-Cycle i160 / i250 CR...
  • Page 168 Steri-Cycle i160 CR Outer dimensions (W x H x D) 637 x 901 x 881 Inner dimensions (W x H x D) 470 x 607 x 576 Inner space volume, approx. 165 of which useable volume approx. 100 Inserts (B x T)
  • Page 169 Sound pressure level (DIN 45 635, part 1) dB(A) < 50 Relative ambient humidity % rH max. 80 Location elevation m above sea max. 2000 level determined on the basis of DIN 12880 for devices with standard feature set. See calibration instructions for detailed information Steri-Cycle i160 / i250 CR...
  • Page 170 Steri-Cycle i160 CR Gas purity min. 99.5 or med. quality Primary pressure min. 0.8 - max. 1 (11.6-14.5 psi) Measurement and control range Vol -% 0...20 Control deviation over time Vol -% ± 0.1 Accuracy at 37 °C and 5% CO ±...
  • Page 171 Circuit breaker 16 A Rated power 230 V: Decontamination: 1.10 Incubation: 0.56 220 V: Decontamination: 0.97 Incubation: 0.51 120 V: Decontamination: 1.01 Incubation: 0.55 100 V: Decontamination: 0.72 Incubation: 0.39 EMV class Steri-Cycle i160 / i250 CR...
  • Page 172 Steri-Cycle i250 CR Outer dimensions (W x H x D) 774 x 964 x 934 Inner dimensions (W x H x D) 607 x 670 x 629 Inner space volume, approx. 255 of which useable volume approx. 162 Shelves - Standard (W x D) 560 x 500 (22.05 x 19.68 inch) Shelf - gas-tight screen option 500 x 400...
  • Page 173 Constant humidity at 37 °C (high humidity mode) % rH approx. 93 Constant humidity at 37 °C (low humidity mode) % rH approx. 90 Sound pressure level (DIN 45 635, part 1) dB(A) < 50 Relative ambient humidity % rH max.
  • Page 174 Steri-Cycle i250 CR Gas purity min. 99.5 or med. quality Primary pressure min. 0.8 - max. 1 (11.6-14.5 psi) Measurement and control range Vol -% 0...20 Control deviation over time Vol -% ± 0.1 Accuracy at 37 °C and 5% CO ±...
  • Page 175 Circuit breaker 16 A Rated power 230 V: Decontamination: 1.26 Incubation: 0.76 220 V: Decontamination: 1.16 Incubation: 0.69 120 V: Decontamination: 1.25 Incubation: 0.75 100 V: Decontamination: 0.89 Incubation: 0.53 EMV class Steri-Cycle i160 / i250 CR...
  • Page 176 Steri-Cycle i160 / i250 CR...
  • Page 177 • “Structure of the Command Sequences for Data Communication” page 12-4 • “Overview of General Parameters (Addresses 0xxx)” page 12-5 • “Overview of Incubator Parameters (Addresses 2xxx)” page 12-5 • “Error Memory Structure” page 12-8 • “Data Logger Structure” page 12-11 •...
  • Page 178 The driver can be run under the following operating systems: WIN 7, WIN 8, WIN 2000, WIN XP, WIN VISTA. Plug the USB cable into the USB interface port (option) in the control box of the Steri-Cycle i160 / i250 and connect with a PC.
  • Page 179 2. Select the Install from a list or specific location (Advanced) option. 3. Select the data CD as source. 4. On the data CD, select the DRIVER sub-directory. 5. The installation routine installs the driver: EVAL22 Board USB. After the installation has been completed successfully, the routine is completed with FINISH.
  • Page 180 The interface transmission speed can be selected within the defined baud rates (9,600, 19,200, 38,400, 57,600 Baud) at the touchscreen of the (see “Settings / Setup” Steri-Cycle i160 / i250 CR page 6-17). All characters sent and received in the data exchange between a PC and the incubator Steri-Cycle i160 / are ASCII characters that can be displayed on a conventional terminal.
  • Page 181 Description of the response elements: aaaa parameter address, amount of payload (always 02) Checksum: CRC8-CCITT: x8 + x2 + x1 + 1 = 0x07 without cc and <CR> XX = 2 bytes error message (see table below) Example of an unknown command: Query: ?:0005:00::cc<CR>...
  • Page 182 2000 Device status (Error-) 33 bytes / hexadecimal value Status of the control loops with the format temperature, xxxxxxxx;xxxx; ... , rH, ref. temp. ;xxxx;xxxx;xxxx 2010 Specified, actual and reference 23 bytes / decimal value temperature with the format +xxx.xx;+xxx.xx;+xxx.xx 2020 Specified and actual CO 15 bytes / decimal value...
  • Page 183 2100 Status of run 25 bytes / decimal value Remaining run time with the format [hours:minutes] xx;+xxx:xx;xx.xx.xx;xx:xx Disinfection, date and time of last start 2105 Status of run , current 25 bytes / decimal value offset + waiting period with the format [minutes:seconds] xx;xx.x;+xxx:xx;xx.xx.xx;xx:xx auto-start, Date and time of last start...
  • Page 184 Note on *3) disinfection and auto-start run status: 0x00 Steri-run not activated auto-start not activated 0x01 Initialization Initialization 0x02 Wait until open door time expires Wait until open door time expires 0x03 Wait until door closes Wait until door closes 0x04 Start Start...
  • Page 185 Example of a response: !:2300:fb:10b01060f372280000002:20b01060f38100001..:80 First data set: !:2300:fb:10b01060f372280000002: (with 21 bytes) Second data set: 20b01060f38100001..:80 (beginning of the second data set after 01060 bytes of the first data set and of the separator [1 byte]) 8000 0002 Type of erroneous control loop Typ fehlerhafter Regelkreis 0x00 = Error in control...
  • Page 186 0x00 Temperature control loop 0x01 control loop 0x02 control loop 0x07 Water level 0x08 General device status 0x0002 Device Door open too long 0x0004 Display fails to communicate 0x0008 Mainboard parameter implausible (EPROM faulty) 0x0010 Data logger defective (unit can continue to function) 0x0020 Error for disinfection / Steri-run 0x0040...
  • Page 187 0x0004 Actual value low 0x0010 Calibration values too high/low 0x0020 Error communication (to sensor) 0x0040 Error communication (to gas cylinder changing unit) 0x0080 No gas, cylinders A and B empty 0x0200 Gas cylinder A empty 0x0400 Gas cylinder B empty 0x0001 Sensor breakage 0x0002...
  • Page 188 Query: ?:2402:00::cc<CR> Repeated output of the most recently read data, this command does not move the reading pointer. This command can be used to avoid data loss after a communication error has occurred. Each query command is responded with up to 7 data sets that follow one another without a separator. These data sets consist of 16 bytes and are encrypted into 32 ASCII characters prior to transmission.
  • Page 189 37 0000 0177 0028 00d4 0000 Type of data logger entry 0x01 = Standard entry which is saved every 60 s Date / Day 0x0b = 11th of months Date / Months 0x01 = January Date / Year 0x06 = 2006 Time / Hours 0x0f = 15 o’clock Time / Minutes...
  • Page 190 0x01 Set values from all servo control Current values for temperature, circuits (periodically in one-minute and rH cycles) 0x02 Set value change (at beginning of Set value for temperature, CO new section) and rH 0x10 Change temperature set value Set value for temperature, CO and rH 0x11 Change CO...
  • Page 191 0x52 auto-start completed with error Status / error registration for temperature, CO and rH 0x53 auto-start stopped manually Status / error registration for temperature, CO and rH 0x60 Start Steri-run Status / error registration for temperature, CO and rH 0x61 Steri-run successfully completed Current actual values for temperature, CO...
  • Page 192 The following code example for reading the data logger uses six functions: • ahex // converts the received ASCII character into a hexadecimal number, • send_telegramm // sends a query to the data logger, • get_telegramm // receives a response data the data logger, •...
  • Page 193 // send telegram ComWrt (COM_NR, string, 14); return; int get_telegramm(char *p) int reading_count = 0; // reading the telegram character by character ComRd(COM_NR, &p[reading_count], 1); // until reception of <CR> while ((p[reading_count++] != ‘\r’)); // return = number of received characters return (reading_count);...
  • Page 194 rest = z % 10; // cut off decimal place z = z / 10; // calculate and copy number before decimal separator for (i = 0; i < 12; i++){ // calculate value a[i] = z%10+0x30; // reduce default value z = z/10;...
  • Page 195 if (!len) { GetError ++; // requery send_telegramm („2402“); len = get_telegramm (buffer); // again, no telegram received if (!len) return 1; // increase telegram counter GetTele ++; // length of sent payload len = (ahex(buffer[7]) * 0x10 + ahex(buffer[8])) / 2; // converting ASCII string into usable numeric string for (i = 0;...
  • Page 196 Entries, Part I:” page 12-14) if (status & DOOR_LONG){ str_cpy (&excelstring[len], „Door open too long;“, 19); len += 19; else { if (status & DOOR_OPEN){ str_cpy (&excelstring[len], „Door open;“, 10); len += 10; // query remaining device errors now · ·...
  • Page 197 // query the remaining events from here case EVENT_FORMAT_DATALOG: WriteFile (FileHandle, excelstring, len); WriteFile (FileHandle, „Data logger erased;\n“,20); break; case EVENT_POWER_ON: // update of set values SetTemp = numeric string [8+i*SIZE_DATA2]*0x100+ numeric string[9+i*SIZE_DATA2]; SollCO2 = numeric string[10+i*SIZE_DATA2]*0x100+ numeric string[11+i*SIZE_DATA2]; SollO2 = numeric string[12+i*SIZE_DATA2]*0x100+ numeric string[13+i*SIZE_DATA2];...
  • Page 198 The information of the service file is very useful for systematic troubleshooting. The data sets are stored in the proprietary format *.SRF. Installing the Steri-Cycle i160 CR Steri-Cycle i250 CR 1. Starting the installation routine: • On the data CD in the subdirectory PROGRAMS, double-click on the file SETUP.EXE to open this file.
  • Page 199 Operating the Steri-Cycle i160 CR Steri-Cycle i250 CR User menu structure: The user surface is divided into two main menus: • MAIN with two functional elements: • Program version output: FIRMWARE VERSION • Switch for exiting the program: QUIT • GENERAL with the submenus: •...
  • Page 200 PRESETTING The submenu PRESETTING is used to set the transmission speed and to select the serial port. 1. Select a transmission speed within the range of 9,600 to 115,200 baud. 2. Select the serial port of the PC. If the USB driver is installed, the (virtual) Com Port assigned to the USB port can be selected (see “USB Interface”...
  • Page 201 TEST COM The submenu TEST COM is used for testing the communication connection with the settings defined in the submenu PRESETTING. 1. Example of a query for the currently measurable temperature values of the incubator: • Query: ? (default, not changeable) •...
  • Page 202 DATE & TIME The submenu DATE & TIME is used for adjusting date and time to the desired time zone. 1. Data in the two text boxes must be entered in the format DD.MM.YY (day, month, year). 2. Accept the input data: •...
  • Page 203 The data sets can be stored in the metaformat *.CSV. 1. Read the data sets: • Press the READ key. 2. The progress of the data transmission is indicated in the three text boxes: • AMOUNT DATA: Total number of transmitted data sets. •...
  • Page 204 PASSWORD The submenu PASSWORD can only be accessed by the service personnel of Thermo Fisher Scientific. Steri-Cycle i160 / i250 CR...
  • Page 205 Please list carried out works here: Device type: Part No.: Serial number: Service number: Location: Operator’s note: Work carried out: Comments: Date Signature Steri-Cycle i160 / i250 CR...
  • Page 206 Steri-Cycle i160 / i250 CR...
  • Page 207 Enquiries from Europe, Middle East and Africa: Phone + 49 (0) 6184 / 90-6940 Fax: + 49 (0) 6184 / 90-7474 Email info.labequipment.de@thermofisher.com Postal address USA: Thermo Scientific 275 Aiken Road Asheville, NC 28804 Enquiries from North America: Phone +1 800-879 7767 +1 800-879 7767 Fax +1 828-658 0363 Email: info.labequipment@thermofisher.com...
  • Page 208 Enquiries at address USA: Thermo Scientific 275 Aiken Road Asheville, NC 28804 Enquiries from USA/Canada +1 866 984 3766 Sales: Service+1 800 438 4851 Enquiries from Latin America +1 866 984 3766 Sales: Service:+1 866 984 3766 Enquiries from Asia:...
  • Page 209 Germany: Postal Address Germany: Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Phone Sales Toll free 0800 1 536 376 or +49 6184 90 6940 Service Toll free 0800 1 112110 or +49 6184 90 6940 E-Mail info.labequipment.de@thermofisher.com Italy +39 02 95059 341 Sales...
  • Page 210 Steri-Cycle i160 / i250 CR...
  • Page 211 The sixfold gas tight screen is a segmented inner door concept designed for the Cell Locker system. The sixfold gas tight screen divides the existing interior of the CO incubator into 6 sample rooms (Cell Locker). In order to be able to remove samples, glass doors (passage openings) are provided in the front plate, which can be opened and closed separately.
  • Page 212 Insert the shelf supports (1) in the lower, center and upper rectangular holes of the support rails. In the upper and center position place the perforated shelves with the unseparated guiding rails. F i g u r e : P e r f o r a t e d S h e l f w i t h U n s e p a r a t e d G u i d i n g R a i l s Do not remove silicone stoppers (1).
  • Page 213 F i g u r e : P e r f o r a t e d S h e l f w i t h S e p a r a t e d G u i d i n g R a i l s f o r t h e L o w e r P o s i t i o n This guiding rails cannot be removed.
  • Page 214 Steri-Cycle i160 / i250 CR...

This manual is also suitable for:

Steri-cycle i250 crHeracell vios 160i crHeracell vios 250i cr