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Summary of Contents for Leica CM1850 UV
- Page 1 Cryostat Instruction Manual Leica CM1850 UV, V1.4 English – 10/2010 Always keep this manual near the instrument! Read carefully prior to operating the instrument!
- Page 3 Leica reserves the right to change technical of-the-art technology as we understand it follow- specifications as well as manufacturing pro- ing thorough investigation in this field.
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Page 4: Table Of Contents
Handwheel assembly ............................16 Setup ............................... 17 Connection to mains power ..........................17 Prior to operation ..............................17 Leica CM1850UV - Overview ..........................18 Mains switch and automatic mains fuse ......................20 Turning the instrument on ..........................20 Control panel operation........................21 Control panel 1 .............................. - Page 5 Error messages in the display ........................... 33 Temperature control button ..........................33 Possible causes and remedies ......................... 34 Cleaning, disinfection, maintenance ....................39 Cleaning ................................39 Spray disinfection with Leica Cryofect ......................40 Maintenance ................................ 41 9.3.1 General maintenance ............................41 9.3.2 Removal of the microtome ..........................
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Page 6: Important Information
Caution – UVC radiation! The Leica CM1850 UV is also suitable for IVD (in vitro diagnostics). Figures in brackets refer to item num-... -
Page 7: Safety
To unlock, push the locking pin (1) to the left from the recess at the handwheel. Only rotate the handwheel when the refrigeration system is on and the cryochamber is cold. Fig. 3 Leica CM1850 UV – Cryostat... -
Page 8: Knife Guard
For current information about applicable standards, please refer to the CE declaration of conformity on our Internet site: www.leica-microsystems.com In order to maintain this condition and to ensure safe operation, the opera- tor must observe the instructions and warnings contained in this instruction manual. -
Page 9: Operating Conditions
Failure to comply with this can cause severe damage to the instrument. Defrosting • The quick freeze shelf may become hot during defrosting! Therefore, do not touch it! Leica CM1850 UV – Cryostat... -
Page 10: Operating The Instrument
• It is not necessary to remove the microtome for routinely disinfecting the cryochamber. The instrument has been designed for UV disinfection. Spray disinfection with Leica Cryofect is also possible, thanks to the spe- cial insulation of the microtome. (Cryofect is not available in all countries!) -
Page 11: Removal Of The Microtome
Technical Service. If any metallic mer- cury is released, handle it carefully and dispose of it properly. If both disinfection indicator lights are blinking alternately, the UV tube must be replaced! Leica CM1850 UV – Cryostat... -
Page 12: Technical Data
Operating temperature range (ambient temperature): 18 °C to 35 °C. All specifications related to temperature are valid only up to an ambient temperature of 22 °C and an air humidity lower than 60 %! Type CM1850 UV CM1850 UV CM1850 UV CM1850 UV... - Page 13 F4: T6.25 A Fa. Schurter, Type FST; 6.3x32 mm or T6.25 A Fa. Littlefuse, Type 313; 6.3x32 mm F5: T4 A Fa. Schurter, Type FST; 6.3x32 mm or T4 Fa. Littlefuse, Type 313; 6.3x32 mm Leica CM1850 UV – Cryostat...
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Page 14: Unpacking And Installation
Unpacking and installation Site requirements Do not operate in rooms with explosion hazard! To ensure an ad- equate cooling capacity, the instrument must be set up with at least 10 cm distance from walls and furniture. • The place of installation must meet the following requirements: –... -
Page 15: Standard Delivery
1 Bottle of cryostat oil, type 407, 50 ml .................... 14 0336 06098 1 Bottle of OCT-Compound, mounting medium for cryosectioning, 125 ml ....... 14 0201 08926 1 Instruction manual Leica CM1850UV (+ language CD) ............14 0471 80002 Compare the delivered components with the parts list and your order. -
Page 16: Handwheel Assembly
Unpacking and installation Handwheel assembly • Insert the pin (1) of the handwheel shaft in to the hole (2) of the handwheel. • Mount the spring washer (3) on the screw (4) as shown in Fig. 16. • Tighten the screw (4) with an Allen key (5 mm). •... -
Page 17: Setup
Open the knife box with the knife and place it in the cryochamber for precooling. • Place all tools needed for specimen preparation in the cryochamber. • Close the sliding window. • Connect the mains plug to the mains power outlet at the wall. Leica CM1850 UV – Cryostat... -
Page 18: Leica Cm1850Uv - Overview
Setup Leica CM1850 UV - Overview Fig. 6 Instruction Manual V 1.4 – 10/2010... - Page 19 14 Thermal bloc (accessory) 15 Section waste tray 16 Brush shelf 17 Orientable specimen head 18 Knife holder base (accessory) 19 Knife holder CE (accessory) 20 Knife holder CN (accessory) 21 Knife holder CE-TC (accessory) Leica CM1850 UV – Cryostat...
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Page 20: Mains Switch And Automatic Mains Fuse
Setup Mains switch and automatic mains fuse The automatic mains fuse is used as mains switch. To turn the automatic mains fuse on, the switch must be set in the upper position (pos. 1). To turn the automatic mains fuse off, the switch must be set in the lower position (pos. -
Page 21: Control Panel Operation
Press UVC key once briefly to start the 30 min mode Press UVC key once for a longer time (approx. 4 sec) to start the 180 min mode Opening the sliding window interrupts the disinfection cycle. Press the UVC key to resume the disinfection cycle! Leica CM1850 UV – Cryostat... -
Page 22: Programming The Desired Values
Control panel operation Programming the desired values 6.2.1 Setting the time The actual time is set on the panel marked with the clock symbol using the keys. When pushing the button for more than 1 second, the time value increases or decreases continuously (autorepeat function). ... -
Page 23: Activation Of The Peltier Element (Option)
To turn off the manual defrosting cycle of the quick freeze shelf prior to the automatic deactivation, press again Fig. 13 Quick freeze shelf and cryochamber defrosting can be run inde- pendently. However, it is not possible to defrost both systems si- multaneously. Leica CM1850 UV – Cryostat... -
Page 24: Manual Defrosting Of The Cryochamber
Control panel operation 6.2.6 Manual defrosting of the cryochamber The manual defrosting of the cryochamber is activated by subsequently pressing the button (audible signal turns on) and the button on the panel for the cryochamber temperature (audible signal turns off). During the defrost cycle, the indication is flashing. -
Page 25: Control Panel 2 - Electric Coarse Feed
The advance movement operates as long as the button is pressed. slow This is a safety feature to protect both the specimen and knife from damage! rapid On reaching the front limit, the LED (2) of the button starts illuminating. Leica CM1850 UV – Cryostat... -
Page 26: Daily Use Of The Instrument
Daily use of the instrument Specimen freezing • Select the appropriate sectioning temperature (cryochamber tempera- ture) for the sample material (please refer to 7.4 Temperature selection chart‘). 7.1.1 Quick freeze shelf The cryochamber has a quick freeze shelf (5, Fig. 17) for up to 10 specimen discs. The temperature of the quick freeze shelf is always lower than the cryochamber temperature. -
Page 27: Specimen Discs
Fig. 18 • Retighten screw (1). 7.2.2 Specimen orientation • To release, loosen screw (4). • Orient the specimen surface with lever (5). • Retighten screw (4). Leica CM1850 UV – Cryostat... -
Page 28: Sectioning
Daily use of the instrument Sectioning All necessary adjustments on the knife holder and anti-roll guide are described in the separate instruction manual for your knife holder in detail. 7.3.1 Trimming the specimen Take care when handling microtome knives and disposable blades. -
Page 29: Temperature Selection Chart (In Minus °C)
Skin without fat Spleenal or bloody tissue Testicular Thyroid Tongue Uterus curettage The temperature values given above are based on long-term experience, however, these are only approximate values, as any tissue may require particular adjustments. Leica CM1850 UV – Cryostat... -
Page 30: Defrosting
Daily use of the instrument Defrosting Defrosting the cryochamber actually means defrosting the evaporator to prevent excessive frost buildup. The evaporator is flushed with hot gas dur- ing defrosting. The cryochamber virtually is frostfree and does not need to be defrosted. The condensation water that is produced during defrosting is collected in a container, which is located at the front of the cryostat cabinet. -
Page 31: Manual Defrosting Of The Quick Freeze Shelf
• Turn out the cryochamber illumination. • Lock control panel 1 (Fig. 8) with the KEY button. • Do not turn the instrument off with the automatic mains fuse as there would be no cooling. Leica CM1850 UV – Cryostat... -
Page 32: Shutdown For A Longer Period
Daily use of the instrument 7.6.2 Shutdown for a longer period If you do not intend to use the instrument for several weeks you may turn it off. Please note, however, that it may take up to several hours to cool the cryochamber down to very low temperatures after turning the instrument on again. -
Page 33: Troubleshooting
--> Clean the ventilation slits (see 9.3.1). Fig. 21 After eliminating the possible source of error, push the temperature control button (1) to turn the in- strument back on. If the instrument fails to turn on, make a service call. Leica CM1850 UV – Cryostat... -
Page 34: Possible Causes And Remedies
Troubleshooting Possible causes and remedies Problem Cause Remedy Frost on chamber walls and mi- - Cryostat is exposed to air cur- - Change place of installation for crotome rents (open windows and the cryostat. doors, air conditioning). - Sliding window was open and exposed to air currents too long. - Page 35 - Very hard, inhomogeneous - Increase section thickness; specimen. reduce specimen surface area if necessary. - Blunt knife/blade. - Use different part of the cutting edge or replace the knife/ blade. Leica CM1850 UV – Cryostat...
- Page 36 8. Troubleshooting Problem Cause Remedy - Knife profile inappropriate for - Use knife with different profile. the specimen to be cut. - Incorrect clearance angle. - Set correct angle. Condensation on anti-roll plate - Brush, forceps and/or cloth are - Store all tools on shelf in the and knife during cleaning too warm.
- Page 37 - Check the mains plug is prop- nected. erly connected. - Defective fuses. - Replace the fuses. - Temperature control switch - Check site conditions as de- activated. scribed in section 5.2, and reset the temperature control switch. Leica CM1850 UV – Cryostat...
- Page 38 8. Troubleshooting Problem Cause Remedy No or insufficient refrigeration - Compressor defective. - Call technical service. - Leak in the cooling system. - Call technical service. - Inappropriate site conditions. - Check site conditions as de- scribed in section 5.2. - Ventilation grilles of the con- - Clean the ventilation slits as denser dirty.
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Page 39: Cleaning, Disinfection, Maintenance
Do not use organic solvents or any other aggressive substances for cleaning and disinfecting! Only use the cleaning agents specified in this instruction manual such as Leica Cryofect (alcohol or com- mon disinfectants based on alcohol)! • Drain the cleaning liquid through the hose after the prescribed reagent time is over and collect it in the waste container (1). -
Page 40: Spray Disinfection With Leica Cryofect
Spray disinfection with Leica Cryofect Basically, we recommend UV disinfection (see page 21 for detailed instruc- tions). For easy-to-use spray disinfection we recommend Leica Cryofect. (Cryofect is not available in all countries!) The cryostat has to be disinfected after each daily use. -
Page 41: Maintenance
To do so, turn the handwheel to place the specimen head to the uppermost position and apply some drops of cryostat oil on to the slot cover; after that place the specimen head to Fig. 24 Leica CM1850 UV – Cryostat... -
Page 42: Removal Of The Microtome
• Do not carry out any repairs on your own as this will invalidate the war- ranty. Repairs may only be carried out by qualified service engineers autho- rized by Leica. The microtome can be removed for thorough cleaning and disin- fecting, or for extensive drying after a long power failure! The germicidal UV lamp is a simple and safe disinfection device which considerably reduces the risk of infection. - Page 43 Fig. 25 • Slightly lift the microtome and pull it to the left to disengage the plastic coupling (5) connect- ing the axes. • Take the microtome out of the cryochamber. Fig. 26 Leica CM1850 UV – Cryostat...
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Page 44: Removal Of The Microtome Cover
Cleaning, disinfection, maintenance 9.3.3 Removal of the microtome cover The microtome cover may be removed to expedite thorough drying of the mi- crotome in an oven. Note: Place the microtome in an oven at 40 °C to 50 °C for several hours. After repeatedly drying the microtome in this manner, it may become neces- sary to relubricate the cross roller... - Page 45 Replace the storage shelves, heat extractor, brush shelf and knife holder in the cryochamber. • Replace the sliding window. Fig. 29 Ensure that all components removed from the cold environment are com- pletely dry before placing them back Fig. 28 into the cryochamber. Leica CM1850 UV – Cryostat...
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Page 46: Replacement Of Uvc Lamp
Cleaning, disinfection, maintenance 9.3.5 Replacement of the fuses Turn the instrument off with the automatic mains fuse and pull the mains plug, before replac- ing the fuses! Only use fuses of the same specification! For the required values, please refer Chapter 3 ‘Technical Data‘. - Page 47 30 seconds, the running-time meter for the UVC lamp is reset. This is required every time the UVC lamp is replaced to Fig. 33 ensure sufficient power for disinfection! Dispose of the UVC lamp separately! Leica CM1850 UV – Cryostat...
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Page 48: Replacement Of The Lamp
9. Cleaning, disinfection, maintenance 9.3.7 Replacement of the lamp • Turn the instrument off with the automatic mains fuse. • Pull the mains plug. • Slightly lift the sliding window (1) holding it by the grip (2) and pull it out to the front. •... -
Page 49: Ordering Information, Optional Accessories
Transfer block, 19 x 75 x 25 mm ..................14 0416 38207 Fixture for specimen clamps, non-orienting ..............14 0458 26771 Thermal block ........................14 0398 18542 Miles-Adapter, for TissueTek specimen discs ............. 14 0436 26747 Leica CM1850 UV – Cryostat... - Page 50 OCT cryoembedding compound, 125 ml ................. 14 0201 08926 Cryostat oil, type 407, 250 ml .................... 14 0336 06100 Leica Cryofect, 4 x 250 ml ....................14 0387 36193 UV tube ..........................14 0471 40422 Instruction Manual V 1.4 – 10/2010...
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Page 51: Optional Accessories
If the cap is too loose, readjust it with the small screw (10). Do not over- tighten the screw. • Once the specimen is removed, take the thermal block out of the cold cryochamber. Fig. 38 Leica CM1850 UV – Cryostat... -
Page 52: Warranty And Service
Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control proce- dure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed char- acteristics warranted. -
Page 53: Decontamination Certificate (Master)
(master) Dear Customer, any product that is to be returned to Leica Microsystems or serviced on site, must be cleaned and decontami- nated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica... - Page 54 Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging or hand it over to the service engineer. Customer as- sumes all responsibility for the immediate return shipment of articles sent to Leica without proper decontamination documentation.
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Page 55: Information For The People´s Republik Of China
13. Information for the People´s Republik of China Leica CM1850 UV – Cryostat... - Page 56 Notice Instruction Manual V 1.4 – 10/2010...
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