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Appendix C: Calibration
Calibration of the LS-AFM requires scanning a standard sample that has known X,Y and Z
dimensions. If a sample with squares is used, the pitch in X and Y should be around 10 µm.
The height of the features in Z should be around 100 nm.
1.
Scan a standard sample with known dimensions in X,Y and Z in both X (0°) and Y
(90°).
2.
Open the AFM Calibration software. Make sure to "allow blocked content."
3.
Insert the current calibration values, located on the System tab, under "System Cal"
in the calibration software.
4.
Insert the known values for the calibration standard in the "Actual" column in the
calibration software.
5.
To calibrate X, open the completed 0° scan in Gwyddion and level the image.
6.
Extract a profile
"Apply."
V 1.1 / LS-AFM Users Guide
over the nicest row of squares, in this case, and click
Appendix C-1
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