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3.
Align the zoom tube such that the hole in
the scanner top plate is directly below the
zoom tube optical objective.
4.
Adjust the height of the zoom tube so that
the bottom of the tube is about 115 mm
from the top of the AFM sample holder.
Move the stage by hand and adjust the
optical focus.
5.
Place a probe in the probe holder (see
section 4.3) and place the probe holder
into the microscope.
6.
With the Manual Z Motor Control, move
the probe such that is about ½ in. above
the sample plate.
7.
With the focus adjustment knob, raise the
zoom tube approximately ½ in.
8.
Slowly move the stage by hand until the
probe is viewed in the video microscope
window. As required, adjust the focus, the
lighting, and the position of the stage until
the probe is in the center of the optical
view.
A.5.2. Aligning the Inverted Microscope with the Cantilever
Viewing the probe through the inverted optical microscope requires moving the the entire
AFM stage until the probe is directly over the optical axis of the inverted microscope. This
alignment should be made with no sample in the microscope sample holder.
1.
Using the Manual Z Motor Control, lower the probe such that it is at the focal plane
of the inverted optical microscope.
2.
Select the lowest magnification available in the inverted microscope turret.
3.
Using the micrometer screws on the back right-hand side of the stage, move the LS-
AFM stage until the probe is visible in the center of the inverted microscope image.
NOTE: The micrometers will move the LS-AFM stage in one direction only.
To move in the opposite direction, the user must manually push the stage.
Appendix A-4
V 1.1 / LS-AFM Users Guide
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