Preparing Samples; Exchanging Samples - AFM Workshop LS-AFM User Manual

4.2 Preparing Samples

Samples can be prepared on glass slides or on petri dishes. It is critical that the samples are
properly adhered to the slides/dishes, to avoid additional vibrations.

4.2.1 Exchanging Samples

Follow the steps below to successfully exchange samples with the LS-AFM.
1. Raise the Z motor until the probe is
at least ¼" from the sample's surface.
2. Remove the probe holder (see sec-
tion 4.3).
3. Remove the thumb screw on the
sample XY positioning stage, and
rotate the sample holder away from
the AFM.
4. Place either the glass slide or the
petri dish in the sample holder.
5. Rotate the sample holder back under
the AFM stage and replace the thumb
screw.
6. Align the sample using the video
optics (see section 4.1).
7. Replace the probe holder with the
probe tip facing the sample.
V 1.1 / LS-AFM Users Guide
Thumb Screw Sample Holder
Section 4-2