Table Of Contents - AFM Workshop LS-AFM User Manual

1. Introduction to Atomic Force Microscopy..................................................................1-1
2. Introduction to the LS-AFM.........................................................................................2-1
2.1. Computer...................................................................................................2-1
2.2. Stage..........................................................................................................2-1
2.3. Ebox..........................................................................................................2-2
2.4. Video Optical Microscope.........................................................................2-2
2.4.1
2.4.2
3. Software..........................................................................................................................3-1
3.1. Installation Procedure...............................................................................3-1
3.1.1.
3.2. AFM-View Software....................................................................................3-2
3.2.1. Pre-Scan Window....................................................................3-2
3.2.3. System Window.....................................................................3-10
3.2.4.
3.3. Video-View Software................................................................................3-14
4. Measuring Images With the LS-AFM..........................................................................4-1
4.1. Operating the Video Microscope.............................................................4-1
4.2. Preparing Samples...................................................................................4-2
4.2.1
4.3. Exchanging Probes...................................................................................4-3
4.4. Aligning the Light Lever Force Sensor...................................................4-4
4.4.1.
4.5. Optically-Assisted Tip Approach.............................................................4-5
4.6. Contact Mode Imaging..............................................................................4-6
Contents
Top View Video Optical Microscope.....................................2-2
Inverted Optical Microscope.................................................2-2
Gwyddion Image Analysis Software......................................3-1
3.2.1.1. Files .........................................................................3-2
3.2.1.2. Modes.......................................................................3-3
3.2.1.3. Laser Align................................................................3-3
3.2.1.4. Tune Frequency........................................................3-3
3.2.1.5. Manual Z Motor Control............................................3-4
3.2.1.6. Range Check............................................................3-4
3.2.1.7. Automated Tip Approach...........................................3-5
3.2.2. Topo Scan Window.................................................3-5
3.2.2.1. Image Window..........................................................3-6
3.2.2.2. Oscilloscope Window................................................3-7
3.2.2.3 Scan Setup...............................................................3-8
3.2.2.4. Z Feedback...............................................................3-8
3.2.2.5. Display......................................................................3-9
3.2.2.6. Tip Retract...............................................................3-10
3.2.2.7. Scan ......................................................................3-10
3.2.3.1. Tip Approach...........................................................3-11
3.2.3.2. Z Parameters..........................................................3-11
3.2.3.3. XYZ Calibration.......................................................3-11
3.2.3.4. XY Parameters........................................................3-12
3.2.3.5. Other Controls.........................................................3-12
Force Distance Curves.........................................................3-13
Exchanging Samples.............................................................4-2
Tips for Aligning the Light Lever Force Sensor..................4-4