Vibrating Mode Imaging; Pre-Scan Tab - AFM Workshop LS-AFM User Manual

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4.7 Vibrating Mode Imaging

These instructions assume the AFM-View software is properly loaded and working, and that
the material presented in section 4 has been reviewed.

4.7.1 Pre-Scan Tab

1.
Follow the instructions in section 4.2. and place a sample in the instrument.
2.
Follow the instructions in section 4.3. and place a vibrating mode probe in the instru-
ment. See Appendix E for probe recommendations.
3.
Focus the video optical microscope on the AFM probe.
4.
Follow the instructions in section 4.4. to align the laser on the probe. Make sure the
Top – Bottom signal is close to 0.0 for vibrating mode imaging.
5.
Select Vibrating in the Scan Mode window.
NOTE: The probe must NOT be in contact with the sample surface dur-
ing Range Check. The piezoelectric ceramics will move to measure the
usable range, which may cause damage to the tip.
6.
If this is the first scan since opening the AFM-View software, initiate the Range Check
function.
7.
Select the frequency under the Tune Frequency window.
a.
Set the Lower limit to the lowest frequency on the probe box.
b.
Set the Higher limit to the highest frequency on the probe box.
c.
Set the Selected value to any frequency between the two limits. If the selected
frequency exceeds either limit, the software will automatically change the values.
d.
Set the number of steps (50-100) and the Demod Gain (4dB).
e.
Set the setpoint in the Automated Tip Approach box to 3000mV to fully retract the
z ceramic, so it does not interfere with the frequency sweep.
f.
Scan the frequency.
g.
Move the green and red cursors toward the resonance peak to decrease the
range, and place the blue cursor corresponding with the selected frequency 80%
up the left side of the resonance peak.
h.
Repeat steps (iv) and (v) until a resonance peak no greater than 2.5V is observed.
If the amplitude of the resonance peak exceeds 2.5V, adjust the driving amplitude
of vibration by altering the Amplitude scroll bar.
i.
For phase mode imaging, the resonance peak must correspond with the slope
of the phase curve. To alter the phase degree, adjust the Phase scroll bar
accordingly.
8.
Focus the video microscope on the sample surface.
9.
Use the Manual Z Motor Control to move the light lever towards the sample (see sec-
tion 4.5.).
10.
Initiate Automated Tip Approach. Wait for the green light to indicate that feedback was
achieved.
V 1.1 / LS-AFM Users Guide
Section 4-10

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