Contact Mode Imaging; Pre-Scan Tab - AFM Workshop LS-AFM User Manual

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4.6 Contact Mode Imaging

These instructions assume the AFM-View software is properly loaded and working, and that
the material presented in section 4 has been reviewed.

4.6.1 Pre-Scan Tab

1.
Follow the instructions in section 4.2. and place a sample in the instrument.
2.
Follow the instructions in section 4.3. and place a contact mode probe in the instru-
ment. See Appendix E for probe recommendations.
3.
Focus the video optical microscope on the AFM probe.
4.
Follow the instructions in section 4.4. to align the laser on the probe. Make sure the
Top – Bottom signal is between 0.4 and 0.8 for contact-mode imaging.
5.
Select Non-Vibrating in the Scan Mode window.
6.
Focus the video microscope on the sample surface.
7.
If this is the first scan since opening the AFM-View software, click on Start in the
Range Check window.
NOTE: The probe must NOT be in contact with the sample surface dur-
ing Range Check. The piezoelectric ceramics will move to measure the
usable range, which may cause damage to the tip.
8.
Use the Manual Z Motor Control to move the light lever towards the sample (see sec-
tion 4.5.).
9.
Initiate Automated Tip Approach. Wait for the green light to indicate that feedback was
achieved.
10.
If the Z Drive indicator is above the center of its range, click the Adv button and use
the Re-Center function. If the Re-Center function does not initiate a new tip approach,
increase the setpoint value by 100mV.
11.
If the Z Drive indicator is below the center of its range, click the Adv button and jog the
motor up by small increments until the indicator is at the center of the Z range.
12.
Once true feedback has been achieved, open the Topo Scan tab at the top of the AFM
Workshop window.
V 1.1 / LS-AFM Users Guide
Section 4-6

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