cytiva Ettan IPGphor Cup Loading Manifold User Manual page 17

Step
1
2
3
Placing paper wicks
Follow the instructions below to place the paper wicks.
Step
1
Ettan IPGphor User Manual 11003460 AE
Action
Place a strip of cups in the appropriate position as shown in the figure below,
for example ~1 cm from the end of the gel portion of the IPG strip. Do NOT
place the cup with the feet over a center protrusion. Push the cups into the
channels with gloved fingers, starting at one end of the strip and working
towards the other.
Align the insertion tool over the cups and push down to ensure that the feet
of the cups are properly seated at the bottom of the channel (wiggle the tool
gently while pushing down in order to ensure that the cups are seated as far
down as they will go). Take care not to move the cups while removing the
insertion tool.
Fill the cups with cover fluid to test for proper seating of the cups. Remove
the cover fluid after 10 minutes.
correct cup position
Note:
Cups must not straddle the centering protrusions on the bottom of the chan-
nels.
Action
Count out the appropriate number of precut paper wicks. Two wicks per strip
are required. Separate the wicks from each other. Add 150 μl distilled water
to each wick.
3 Operation
3.1 Instructions
incorrect cup position
17