cytiva Ettan IPGphor Cup Loading Manifold User Manual page 11

IEF electrode paper wicks
The small, precut rectangular wicks, shown below, must be placed at both the anodal
and cathodal ends of the rehydrated IPG strips just under the electrodes. The wicks
absorb excess water, salts, and proteins with pIs that lie outside the pH range of the IPG
strip.
Paper bridge loading pads
Paper bridge loading pads, as shown below, are used to load large volumes of sample
(375–500 μl). The paper bridges are 0.8 cm × 5.0 cm in size with pointed ends. One end
of the pad contacts the IPG strip and is held in place by the hold down teeth of the elec-
trode assembly. The other end of the paper bridge loading pad makes contact with a
paper wick placed under the platinum wire of the electrode assembly.
Ettan IPGphor User Manual 11003460 AE
2 Description
2.1 Overview
11