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Summary of Contents for cytiva Filter Kit HR 10
- Page 1 Filter Kit HR 10, Filter Kit HR 16 Instructions for Use These instructions describe how to change filters and O-rings on HR 10 and HR 16 columns. cytiva.com 52177700 AN...
- Page 2 Table of Contents Filter kit contents ................... 3 When to change the filter ..............3 How to change the filter ............... 3 Troubleshooting ..................7 52177700 AN...
- Page 3 1 Filter kit contents These filter kits (Code Nos. 18357501/18358501 respectively) contain 10 filters and 4 O-rings. 2 When to change the filter Change the filter on the top of your column when you observe an increased backpressure, a space which has become visible between adapter and filter, a loss of resolution and always before you perform a thorough column cleaning.
- Page 4 Step Action Pull the adapter out by the red ring. Disconnect the column top inlet tubing from the valve. Check that some buffer remains over the chromatography medium bed. Carefully and slowly bring up the loose filter by using the hook (part of the Filter tool).
- Page 5 Step Action Wet a new filter in 20% ethanol. Put the filter into the column, avoiding air bubbles under it. Push it gently down to the medium surface with the plunger. For HR 16 columns, use the wider end of the plunger. Note: As the filter is inserted, any medium which has been stirred up will be...
- Page 6 Step Action Adjust the adapter to the medium bed by clockwise rotation of the red adjusting ring until there is no space visible between the adapter and the filter. Check that no large air bubbles have been trapped between the medium and the adapter.
- Page 7 Use a flow rate of 1 to 1.5 to ml/min for 10 to 30 to minutes. For further information contact your local Cytiva representative. 52177700 AN...
- Page 8 Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco LLC or an affiliate. All other third-party trademarks are the property of their respective owners. © 2020 Cytiva – All rights reserved. All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business.
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