Instructions - cytiva Ettan IPGphor Cup Loading Manifold User Manual

3 Operation

3.1 Instructions

3.1 Instructions
Rehydrating the IPG strips
Follow the instructions below to rehydrate the IPG strips.
Step
1
2
3
Placing the Manifold on the IEF unit
Follow the instructions below to place the manifold on the IEF unit.
Step
1
14
Action
Rehydrate the IPG strips with the gel side down in the appropriate volume of
rehydration solution using the Immobiline DryStrip Reswelling Tray.
Rehydration in the Cup Loading Manifold is not recommended: the channel
is too wide to ensure proper rehydration.
Follow the instructions included with the Immobiline DryStrips. The table
below is provided as a reference.
IPG strip length (cm)
7
11
13
18
24
Cover the IPG strips with Immobiline DryStrip Cover Fluid and allow the
strips to rehydrate overnight (10–20 hours).
Action
Clean and dry the IPGphor bed before placing the manifold on the unit.
Position the manifold on the IPGphor platform.
Rehydration volume (μl)
125
200
250
340
450
Ettan IPGphor User Manual 11003460 AE