Molecular Devices FlexStation 3 Reader Manuals

Molecular Devices FlexStation 3 Reader Manuals

Manuals and User Guides for Molecular Devices FlexStation 3 Reader. We have 1 Molecular Devices FlexStation 3 Reader manual available for free PDF download: User Manual

Molecular Devices FlexStation 3 User Manual

Molecular Devices FlexStation 3 User Manual (173 pages)

Benchtop Multi-Mode Microplate Reader

Brand: Molecular Devices | Category: Card Reader | Size: 8 MB

Table of Contents

Table of Contents
3
Chapter 1: Description

7
- Introduction
  7
- How to Use this User Guide
  9
  - User Guide Organization
    9
- Safety Information
  9
- System Overview
  13
- System Components
  15
  - Covers and Instrument Panels
    17
  - Drawers
    21
  - Fluidics Module
    24
  - Detection Module
    26
  - Computer
    28
  - Accessories
    28
  - Consumables
    30
- Overview of Operation
  32
  - Choosing an Experiment
    32
  - Preparing the Instrument
    32
  - Preparing the Software
    33
  - Running the Experiment
    33
  - Analyzing the Data
    33
- Theory of Operation
  33
  - Instrument Design
    33
  - Assay Read Types
    38
- Softmax Pro Software
  41
- Flexstation 3 Benchtop Multi-Mode Microplate Reader User Guide
  48
Chapter 2: Installation

49
Chapter 3: Operating Procedures

65
- Overview
  66
- Starting up the System
  67
  - Using the Control Panel
    70
- Setting the Temperature
  72
- Setting up the Software
  75
- Softmax Pro Software Parameters
  78
  - Read Types
    79
  - Read Modes
    79
  - Wavelengths
    80
  - Sensitivity
    80
  - Timing
    81
  - Assay Plate Type
    82
  - Wells to Read
    83
  - Automix
    84
  - Autocalibrate
    85
  - Settling Time
    85
  - Autoread
    85
- Softmax Pro Software Parameters for Fluid Transfer
  86
  - Compound Source
    87
  - Compound Transfer
    88
  - Triturate Selection
    92
  - Pipette Tips Layout
    93
  - Compound and Tip Columns
    93
  - Pipette Tip Air Gap
    97
- Settings Displayed in Plate Sections
  98
- Other Software Settings
  99
- Reading a Microplate
  99
  - Loading Tips and Microplates
    99
  - Starting the Reading
    100
  - Selecting a Plate
    101
  - Replacing Data in a Plate
    101
  - Viewing Experiment Progress
    102
  - Data Display
    102
- Shutting down the System
  103
- Optimizing Fluorescence Assays
  103
  - Using Spectral Scanning to Optimize Excitation and Emission Wavelengths for Fluorescence Assays
    105
- Optimizing Time-Resolved Fluorescence Assays
  110
- Optimizing Fluorescence Polarization Assays
  111
- Optimizing Luminescence Assays
  112
Chapter 4: Maintenance

113
- Obtaining Support
  114
- Moving the Instrument
  114
- Cleaning the Instrument
  115
  - Cleaning up Spills
    116
  - Cleaning the Fan Filter
    116
  - Cleaning the Barrels on the Pipettor Head
    117
- Using the Microplate Adapters
  120
  - Microplate Adapter Installation
    120
  - Removing the Microplate Adapter
    120
- Using the Compound Baseplate
  121
- Replacing Fuses
  121
- Replacing the Flash Lamp
  123
- Long-Term Shutdown
  128
Chapter 5: Troubleshooting Procedures

129
- Problems During Startup
  130
- Opening a Drawer Manually
  132
- Understanding Potential Mechanical Problems
  133
  - Background
    133
  - Before Using the Instrument
    133
  - Avoiding Mechanical Problems
    134
  - In Case of Power Failure
    135
- Fluidics
  135
- General Error Messages
  147
  - Error Messages
    147
  - Other Error Messages
    148
- Tilting or Removing the Fluidics Module
  149
  - Tilting the Fluidics Module
    149
  - Removing the Fluidics Module
    151
Appendix A: Parts and Accessories

153
- Ordering Parts and Accessories
  154
Appendix B: Performance Specifications

155
Glossary

163
Index

167

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