Operating Method - Thermo Scientific HistoStar Operator's Manual

HistoStar Operator Guide A81010100 Issue 2

Operating Method

Note
The embedding procedure outlined below is a general method only. Different commercial moulds and cassettes
may require additional or different steps which are usually specified by the supplier. Ensure that any additional
steps are incorporated into this procedure and that common sense is used in all cases.
• Turn on HistoStar and allow the temperatures of both modules to stabilise - see Switching On HistoStar.
• Place the processed tissue cassettes in the tissue storage tank.
Note
Ensure that melted wax covers all of the cassettes.
• Remove a cassette from the tissue storage tank and remove the lid - the lid can be stored in the cassette lid
storage dish.
Note
When storing cassettes in the tissue storage tank, it is recommended that they be kept in an organizer basket.
Place the cassette on one of the hot areas in front of the tissue storage tank.
•
• Select a suitable clean base mould from the storage compartment and place it on the hot spot below the wax
dispenser.
Push the dispense lever backwards to fill the base mould to a level suitable for tissue orientation.
•
Do not allow a skin to form on the surface of the wax inside the base
mould. This can cause the wax to separate during microtomy.
Note
Wax can also be dispensed using the footswitch.
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