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UVP Minidizer
Hybridization Oven
Instruction Guide
___________________________________________________________________
Analytik Jena US
Ultra-Violet Products Ltd.
2066 W. 11th Street, Upland, CA 91786
Unit 1, Trinity Hall Farm Estate
Tel: (909) 946-3197
Nuffield Road, Cambridge CB4 1TG UK
Fax: (909) 946-3597
Tel: +44(0)1223-420022 Fax: +44(0)1223-420561
Web Site: www.us.analytik-jena.com
81-0169-02 Rev J
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Summary of Contents for Endress+Hauser Analytik Jena UVP Minidizer
- Page 1 UVP Minidizer Hybridization Oven Instruction Guide ___________________________________________________________________ Analytik Jena US Ultra-Violet Products Ltd. 2066 W. 11th Street, Upland, CA 91786 Unit 1, Trinity Hall Farm Estate Tel: (909) 946-3197 Nuffield Road, Cambridge CB4 1TG UK Fax: (909) 946-3597 Tel: +44(0)1223-420022 Fax: +44(0)1223-420561 Web Site: www.us.analytik-jena.com 81-0169-02 Rev J...
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Page 2: Table Of Contents
UVP Minidizer Hybridization Oven Table of Contents Introduction ................................... 3 Hybridization Oven Specifications..........................4 Installation ..................................5 Operation ..................................6 Service Procedures ................................. 6 Hybridization Techniques ............................10 81-0169-02 Rev J... -
Page 3: Introduction
UVP Minidizer Hybridization Oven Introduction The UVP Minidizer Hybridization Oven is a cost-effective, personal desktop hybridization oven with a capacity of four 35x150mm bottles, eight 50ml or 15ml conical plastic tubes. The Minidizer operates with a state-of-the-art microprocessor as well as temperature and variable speed controls. The Minidizer enables consistent saturation of samples, whether it for washing or hybridizing. -
Page 4: Hybridization Oven Specifications
UVP Minidizer Hybridization Oven Hybridization Oven Specifications UVP Minidizer Hybridization Oven Part Number Volts/Hz 95-0330-01 115V/60Hz 95-0330-02 230V/50Hz 95-0330-03 100V/50Hz Specifications Net Weight: 11.3 lbs (5 kg) Temperature: Ambient +10°C to 80°C Heating Element: 500 watts Temperature Display: Rotation Speed: 12 RPM Bottle Capacity: Four (4) 35x150mm, Eight (8) 50ml, or Eight (8) 15ml... -
Page 5: Installation
UVP Minidizer Hybridization Oven Installation The Minidizer Hybridization Oven has capacity for four 35x150mm bottles and eight 50ml or 15ml conical plastic tubes. Combinations of multiple-sized bottles may be used at the same time. To insert bottles or tubes into the carousel, gently press them into the clips. -
Page 6: Operation
UVP Minidizer Hybridization Oven Operation Caution: This equipment is not intended for interconnection with any other devices. Use of this equipment other than as intended may create a safety hazard and/or malfunction. Using the Hybridization Oven Place the unit on a level working surface and provide adequate room in front of the door to allow easy opening. Plug the female end of the power cord into the back of the unit and the male end into a surge-protected outlet. - Page 7 UVP Minidizer Hybridization Oven The units are built to provide trouble-free operation for the life of the system. To ensure correct operation: Wipe any water from inside and outside the unit with a soft cloth or sponge. • Use soap and water with a soft cloth or sponge to clean the unit. •...
- Page 8 UVP Minidizer Hybridization Oven 68-0088-01 Carousel Pillow Block 1 Required 46-0024-02 Motor Drive Coupler Assembly 1 Required 56-0022-01 Fuse, 3AMP/250V 5x20mm (Sold Individually) 2 Required 88-0004-01 Bottle Cap and O-Ring/PTFE Seal 07-0194-01 Borosilicate glass bottle with polypropylene cap and PTFE seal, 35 x 150 mm 58-0085-01 Power Cord (115V) 58-0085-03...
- Page 9 UVP Minidizer Hybridization Oven Warranty Analytik Jena’s quality hybridization ovens are guaranteed to be free of defects in materials, workmanship and manufacture for one (1) year from the date of purchase. Consumable and disposable parts including, but not limited to bottles, tubes and filters, are guaranteed to be free from defects in manufacture and materials for ninety (90) days from the date of purchase.
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Page 10: Hybridization Techniques
UVP Minidizer Hybridization Oven Hybridization Techniques There are really two main steps to a hybridization reaction: hybridizing two strands of complementary DNA, and detection of the hybridized DNA. Nucleic acid hybridization is a mechanism where strands of DNA in a single stranded state have their complements bind together. - Page 11 UVP Minidizer Hybridization Oven 3. Salt Concentration and Temperature Nucleic acid requires salt (monovalent cations) to reduce the ionic effects of the phosphate backbone, and heat as a form of non-denaturing kinetic energy. Because the salt concentration and temperature effect each other, knowing the thermostability of the hybrid probe is helpful. Hybridization rate varies directly with the sodium ion concentration between 0.03 and 1.2 M.
- Page 12 UVP Minidizer Hybridization Oven Protocol 1: Random Priming Method for Tagging DNA with Fluorescein-Labeled Nucleotide and Others This method uses DNA polymerase to incorporate Fluorescene-11- dUTP into double stranded DNA probes. This protocol can be used to incorporate any tagged nucleotides. Equipment Micropipettes and tips •...
- Page 13 UVP Minidizer Hybridization Oven Protocol 2: Hybridization to Nylon or Nitrocellulose Hybridization to nylon or nitrocellulose membranes containing Nucleic Acid is accomplished by adding single- stranded probe to the membranes that have been previously incubated with prehybridization solution. The prehybridization and hybridization solutions both contain buffers designed to prevent adventitious binding of the probe to the filters.
- Page 14 UVP Minidizer Hybridization Oven Non-Radioactive Probes Wash blots in 2X SSC, 0.1% SDS for 3 minutes at room temperature (repeat one) Wash filter in 0.15X SSC, 0.1%SDS for 15 minutes at 50 °C (repeat once) Store blots in 2X SSC at room temperature Radioactive Probes Additionally you will need: X-Ray film holder...
- Page 15 UVP Minidizer Hybridization Oven Protocol 3: Chemiluminescence Detection: HRP-Tagged, Alkaline Phosphatase (AP) Probes or Antibody Conjugates Equipment Clear plastic cling-wrap or Clear transparent sheet protector • UVP EC3 or AC1 Darkroom with Cooled CCD camera • Pipette • Reagents for Chemiluminescence ECL™...
- Page 16 UVP Minidizer Hybridization Oven 81-0169-02 Rev J...
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