Tripod Mounting; Temperature And Humidity; Determining Results - Cantium Scientific MicroBio MB2 Operating Manual

Tripod Mounting

The MicroBio MB2 incorporates a standard
¼"-20 UNC tripod mount, as defined in
ISO1222:2010. Tripods designed for most
photographic equipment are compatible
with the MicroBio MB2.

Temperature and Humidity

It is useful to measure these at the time of each sample. Temperature and
humidity are important factors in the likely concentration and viability of
airborne micro-organisms. For example, some bacteria survival rates are 35
to 65 times higher at 80%RH compared to 40%RH.

Determining Results

Once the sample has been taken, the contact plate or petri dish should be
removed and sealed with its protective lid. A note should be made on the lid
regarding time, location, volume sampled etc., and stored appropriately.
The plate should be incubated for a period of time and temperature
dependant on the requirements of the media.
Once incubated, the colony growths are then counted, either manually or
using an automatic colony counter. Due to the statistical nature of the
sampling method and the chance more than one colony impinged at one
point on the dish, a count correction needs to be performed.
The tables in Appendix A and Appendix B give the corresponding
corrections for the 220 and 400 hole sampling heads respectively for
uncorrected counts up to 160 CFU.
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