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Related Manuals for Cantium Scientific MicroBio MB1
Summary of Contents for Cantium Scientific MicroBio MB1
- Page 1 MicroBio MB1 Bioaerosol Sampler Operating Manual...
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Page 2: Operating Manual
MicroBio MB1 Bioaerosol Sampler Operating Manual This work is licensed under a Creative Commons Attribution, Non-Commercial, ShareAlike 4.0 International License. https://creativecommons.org/licenses/by-nc-sa/4.0/ Cantium Scientific Limited Clarendon Gardens Dartford Kent DA2 6EY United Kingdom Tel: +44 (0) 1322 252000 www.cantiumscientific.com ... -
Page 4: Table Of Contents
Contents Regulatory Compliance ................... Warranty ......................MicroBio MB1 Technical Specification ............. Introduction ...................... Installing Battery ....................Sampling ......................Selection of Media ................... IQ / OQ / PQ ....................Inserting a Contact Plate or Petri Dish ............Switching On and Off .................. - Page 5 Appendix E - Bioaerosols ................What is a bioaerosol? ..................Sources of Bioaerosols ..................Monitoring of Bioaerosols ................
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Page 6: Regulatory Compliance
Regulatory Compliance EC Declaration of Conformity This is to certify that MicroBio MB1 products manufactured from April 2014 meet the following European Community Directives: Electromagnetic Compatibility (EMC) Directive 89/336/EEC repealed by 2004/108/EC to the following standard: EN 61326-1 Scientific, Test and Measurement Equipment Referencing: ... - Page 7 2003/108/EC. Outside of the European Community, consult local regulations or your local distributor. The MicroBio MB1 enclosure and fan housing is manufactured from ABS plastic. The petri dish support plate and fan body are manufactured from glass reinforced nylon.
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Page 8: Warranty
Warranty The manufacturer warrants this product to be free from defects in materials and workmanship for 24 months from the date of purchase. If your product is found to be defective within that period, please contact Cantium Scientific Limited or your local distributor who will arrange for repair of the instrument, or if necessary a replacement. -
Page 9: Microbio Mb1 Technical Specification
Technical Specification MicroBio MB1 Flow Rate: 100 L.min -1 ‡ Sample Volume: 10 to 2,000 litres in varying steps Sampling Volume Capacity: ~ 60,000 litres before recharge* 1.7 µm (220 x 1mm hole head) d50 Particle size: 1.35 µm (400 x 0.7mm hole head) 9.62 ms... -
Page 10: Introduction
Introduction The MicroBio MB1 is part of the MicroBio range of bioaerosol samplers and is one of the most economical hand-held samplers in the world for monitoring airborne micro-organisms or bioaerosols. The MicroBio range meets the standard required for a reference sampler, as fully validated by the UK Department of Trade and Industry Validation of Analytical Methods (VAM) programme. -
Page 11: Installing Battery
Installing Battery The battery is held in a compartment at the back of the MicroBio MB1. The unit is supplied with 4 x AA NiMH rechargeable cells. The cells must be removed for re-charging. To open the battery compartment press firmly on the “OPEN” marking on the battery compartment lid near the serial number label and then slide downwards. -
Page 12: Sampling
Sampling The selection of the sample volume is important for reliable sampling. If the contact plate or petri dish is overloaded with colonies it is difficult to make an accurate count. With experience, the user will anticipate the probable bioaerosol concentration in an area, but it may be necessary to make a preliminary survey at a number of sampling volumes to identify the optimum setting. -
Page 13: Inserting A Contact Plate Or Petri Dish
Inserting a Contact Plate or Petri Dish Carefully remove the sampling head cover from the MicroBio MB1, unless it is being carried separately in a sterile container. Only hold the edge of the head. Do not touch the perforated or inside surfaces. -
Page 14: Switching On And Off
When the battery needs re-charging the display will show four dots below the volume setting. If the battery level is too low, the MicroBio MB1 will not allow samples to be taken. -
Page 15: Starting / Stopping Samples
When running the display will countdown showing how many litres remain to be sampled. The MicroBio MB1 will stop sampling when the required volume of air has been sampled. Press and hold the stop button at any time to stop sampling. -
Page 16: Temperature And Humidity
Temperature and Humidity It is useful to take a note of these values at the time of each sample. Temperature and humidity are important factors in the likely concentration and viability of airborne micro-organisms. For example, some bacteria survival rates are 35 to 65 times higher at 80%RH compared to 40%RH. Determining Results Once the sample has been taken, the contact plate or petri dish should be removed and sealed with its protective lid. - Page 17 If the counted colonies, (n ) exceeds the number of sampling head holes ), then the equation will fail and the results cannot be trusted. If this is the case, then the sample dish can be considered as overloaded with micro- organisms and the user should consider lower sampling volumes.
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Page 18: Change Plate / Dish Type
Change Plate / Dish Type The MicroBio MB1 can accommodate both 55mm / 65mm contact plates or 90mm petri dishes. The MicroBio MB1 is factory fitted with the petri dish springs, but these can be removed and replaced with the supplied contact plate springs (Cantium Scientific Limited part number P0001M007). -
Page 19: Cleaning
All equipment should be handled aseptically. The MicroBio MB1 is suitable for use with Hydrogen Peroxide Vapour (H vapour) bio-decontamination. As the MicroBio MB1 has a fan within the... -
Page 20: Validation
Validation Some industries require sampling equipment to be validated before being used. For the MicroBio MB1 this can be achieved using the MicroBio Validation Kit, part number P0059 available from Cantium Scientific Limited or your local distributor. Calibration It is recommended the MicroBio MB1 is regularly calibrated in accordance with specific industry best practice. ... -
Page 21: Troubleshooting
Troubleshooting The MicroBio MB1 will give many years of trouble-free service with minimal routine maintenance. However, below are some common questions and answers relating to the use of the MicroBio MB1 Bioaerosol Sampler. Q. The unit will not switch on. ... -
Page 22: Appendix A - 220 Hole Count Correction Table
Appendix A - 220 Hole Count Correction Table Count Corrected Count Corrected Count Corrected Count Corrected... -
Page 23: Appendix B - 400 Hole Count Correction Table
Appendix B - 400 Hole Count Correction Table Count Corrected Count Corrected Count Corrected Count Corrected... -
Page 24: Appendix C - Culture Media Types
Appendix C - Culture Media Types Incubation Micro-organism Agar Culture Medium Temperature Bacteria: Human Flora Blood Agar 35 - 37°C Heart Infusion Agar Possible Pathogens 35 - 37°C Soya bean-casein digest agar (SCDA) Environmental saprophytic SCDA or R2A 25 - 30°C Thermophylic EMB or Endo Agar 35 - 37°C... -
Page 25: Appendix D - Replacement Parts
Appendix D - Replacement Parts HEAD RETAINING SPRING (PART NO. P0001M009) Replacement sampling head retaining spring. Sold individually Head Retaining Spring CONTACT PLATE HOLDING SPRINGS (PART NO. P0001M007) Replacement springs used to hold 55 mm contact plates. 3 per pack. Contact Plate Springs PETRI DISH RETAINING SPRINGS (PART NO. - Page 26 SPRING RETAINING SCREWS (PART NO. P0001SCR003) Replacement stainless steel M3 x 6 screws used to fasten the dish, plate and head retaining springs. Sold in a pack of 10. MicroBio Head Screws...
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Page 27: Appendix E - Bioaerosols
Appendix E - Bioaerosols What is a bioaerosol? Bioaerosols are airborne particles, solid or liquid. They can be large molecules or volatile compounds. They contain living organisms. They will vary in size from a fraction of a micron to around 100 microns. As with inert “dust”... -
Page 28: Monitoring Of Bioaerosols
The simplest quantitative method of monitoring is to use impact samplers such as the MicroBio MB1 or MB2 units. These are single stage sieve impactors, which collect bacteria and fungal spores from air flowing at 100 litres per minute through a series of air inlets, onto an agar filled 55 mm... - Page 29 NOTES:...
- Page 30 Cantium Scientific Limited MicroBio MB1 Bioaerosol Sampler Operating Manual www.cantiumscientific.com October 2016...