Sampling; Selection Of Media; Iq / Oq / Pq - Cantium Scientific MicroBio MB2 Operating Manual

Sampling

The selection of the sample volume is important for reliable sampling. If the
contact plate or petri dish is overloaded with colonies it is difficult to make an
accurate count. With experience, the user will anticipate the probable
bioaerosol concentration in an area, but it may be necessary to make a
preliminary survey at a number of sampling volumes to identify the optimum
setting. Each sample should be repeated several times and a statistical
mean value and confidence value determined.

Selection of Media

The agar media used in the contact plate or petri dish should be chosen to
suit the micro-organisms being monitored. For a wide range of micro-
organisms, consider using tryptone soy agar (TSA), casein soy peptone agar
(CPSA) or nutrient agar (NA). There are other selective agars for more
specific micro-organisms. For fungi (yeasts and moulds), consider using malt
extract agar (MEA) or rose bengal agar (RBA). Appendix C details various
culture media types.

IQ / OQ / PQ

Documentation templates are available from Cantium Scientific Limited or
your local distributor to support in-house Installation, Operational and
Performance Qualification.
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