Thermo Scientific invitrogen EVOS M5000 Manual page 58

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8. Click the Browse button to open Choose Save Folder dialog.
9. Go to the destination folder for the Z Stack images, then click Select Folder.
10. Select the File Type: PNG or TIFF.
11. Select Generate Projection Image to generate a projection image.
When selected, the system uses the most in-focus pixels from images captured
at different focal planes to generate a composite in-focus image. Each image in
the Z-Stack is also separately saved.
When unselected, the system only saves the individual Z-Stack images.
12. Click Start to capture the Z-Stack image sets based on your specifications.
The system provides information about the progress of the Z Stack protocol.
EVOS M5000 Imaging System User Guide
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