Thermo Scientific invitrogen EVOS M5000 Manual page 47

EVOS M5000 Imaging System User Guide
™
10. Define the Z-Stack parameters to determine the number of "optical sections"
captured in the Z-Stack image set:
•
Automatically compute Z-Stack Parameters: Select to calculate the
number of images and their Z-positions automatically based on the
Z-Stack boundaries and the default Step Size.
•
Manually Set Step Size: Select to enter the Step Size (Z-distance in µm
between focal planes) in the corresponding text box. The system calculates
the number and Z-positions of the images based on your entry.
•
Manually Set Number of Images: Select to enter the Number of Images
(Z-distance in µm between focal planes) in the text box. The system
calculates the Step Size and the Z-positions of the images based on the
number of images.
11. Select Generate Projection Image to generate
a projection image.
When selected, the system uses the most in-focus pixels from images captured
at different focal planes to generate a composite in-focus image. Each image in
the Z-Stack is also separately saved.
When unselected, the system only saves the individual Z-Stack images.
12. Click Next to set up a Run.
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