Thermo Scientific invitrogen EVOS M5000 Manual page 35

EVOS M5000 Imaging System User Guide
™
9. The Refine section displays a histogram plot showing Count versus Intensity,
Area, or Circularity. In this example, Area is selected.
10. To refine your count, select Intensity, Area, or Circularity, move the gate
handles to set the upper or the lower boundary for the selected parameter.
You can refine the count by a single parameter or by multiple parameters.
The software applies the selected boundaries and recalculates the count.
11. When finished with the count, save your count results (see "Save analysis
results", page 38).
IMPORTANT! If you navigate away from the Auto Count screen, your count will
be lost. To preserve the count results, make sure to save it before you navigate
away from the count screen.
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