Thermo Scientific invitrogen EVOS M5000 Manual page 39

EVOS M5000 Imaging System User Guide
™
4. Click Target, then click and drag on the image to draw a
circle around an area that contains cells (green circle).
5. Click Background, then click and drag on the image to
draw a circle around a background area that does not
contain any cells (red circle).
Note: You can select up to 5 targets and 5 background areas. Increasing the
number of targets and background areas improves the accuracy and
reproducibility of the confluence measurements.
6. Refine the sensitivity of the Confluence measurement using the Sensitivity
slider. Decreasing the sensitivity reduces the confluence value.
7. The software automatically calculates the confluence of your culture and
displays the results as a percentage of confluence.
8. When finished with the confluence measurement, save your results (see "Save
analysis results", page 38).
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