Thermo Scientific invitrogen EVOS M5000 Manual page 33

EVOS M5000 Imaging System User Guide
™
4. If needed, click Target again to identify further objects
to include in your count (for example, targets that might
appear different).
Note: For best results, follow these guidelines when identifying target objects:
•
When selecting objects, circle the entire object and include a slight border
around it.
•
To include objects of lower intensity in your count, select dimmer objects
during identification.
•
Circle only one object at a time to help define object size for segmentation.
5. To distinguish the target from background, click
Backround, then click and drag to draw a circle (red) in
a background area.
6. To count closely grouped cells that are touching or overlapping as distinct
objects, select from the Split Cells options:
•
None: Touching or overlapping objects are not counted separately.
•
Shape: Distinct objects are identified and counted based on shape.
•
Intensity: Distinct objects are identified and counted based on pixel
intensity.
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