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Thermo Scientific TriPlus 500 User Manual

Thermo Scientific TriPlus 500 User Manual

Headspace sampler
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Thermo Scientific
TriPlus 500
User Guide
Headspace Sampler
P/N 31716106 Revision A
January 2019

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  • Page 1 Thermo Scientific TriPlus 500 User Guide Headspace Sampler P/N 31716106 Revision A January 2019...
  • Page 2 © 2019 Thermo Fisher Scientific Inc. All rights reserved. Thermo Scientific™ TriPlus™ 500 Headspace Sampler and TRACE™ 1300 Series GC (TRACE™ 1300 GC, TRACE™ 1310 and TRACE™ 1300E) are trademarks of Thermo Fisher Scientific; VICI ® is a registered mark of the Valco Instrument Co.
  • Page 3 Reader’s Survey TriPlus 500 Headspace Sampler User Guide, PN 31716106, Revision A Strongly Strongly Agree Neutral Disagree Agree Disagree The manual is well organized. The manual is clearly written. The manual contains all the information I need. The instructions are easy to follow.
  • Page 5 – FCC rules: CFR no. 47 Part 15 Subpart B Section 15.107 and 15.109 Safety – EN 61010-1:2010, IEC 61010-1:2010 – EN 61010-2-010: 2014, IEC 61010-2-010: 2014 (TriPlus 500 HS only) – EN 61010-2-081: 2015, IEC 61010-2-081: 2015 – UL 61010-1:2012/R:2016-04...
  • Page 6 – UL 61010-2-081:2015 – CAN/CSA C22.2 No. 61010-1:2012/U2:2016-04 – CAN/CSA C22.2 No. 61010-2-010:2015 (TriPlus 500 HS only) – CAN/CSA C22.2 No. 61010-2-081:2015 Note This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.
  • Page 7 Notice on the Susceptibility to Electromagnetic Transmissions Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument.
  • Page 9 WEEE Directive 2012/19/EU Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product. See www.thermofisher.com/ rohsweee for further information on Thermo Fisher Scientific compliance with these Directives and the recyclers in your country.

  • Page 11: Table Of Contents

    Chapter 1 Getting Familiar with Your TriPlus 500 Headspace Sampler ....1 Introduction ........... . 2 Instrument Basics .
  • Page 12 TriPlus 500 Web Interface ........
  • Page 13 Using the TriPlus 500 Web Interface ........
  • Page 14 Manufacturing Menu ......... 116 Logging Out of the TriPlus 500 Web Interface ......116 Chapter 8 Ordering Parts .

  • Page 15: Preface

    This guide contains detailed information for the use of the Thermo Scientific™ TriPlus™ 500 Headspace Sampler (TriPlus 500 HS). This guide is intended for frequent or new TriPlus 500 HS users who are experienced at using automated systems to run existing analytical methods.

  • Page 16: About Your System

    Input 100-240 Vac; 50/60 Hz; 1.3 A — Output 24 Vdc; Power 90 W; 3.75 A WARNING You must only use the portable external power supply provided with the instrument by Thermo Fisher Scientific. Detailed instrument specifications are in the Product Specifications Sheet. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 17: Contacting Us

    In addition to this guide, Thermo Scientific™ provides the following documents for TriPlus 500 HS. • TriPlus 500 Headspace Sampler Preinstallation Requirements Guide, PN 31716105 • TriPlus 500 Headspace Sampler Hardware Manual, PN 31716107 • TriPlus 500 Headspace Sampler Spare Parts Catalog, PN 31716108 To suggest ways we can improve the documentation, follow this link to complete our Reader’s...

  • Page 18: Safety Symbols And Signal Words

    HARMFUL. Indicates that the presence of harmful material will, could, or might occur. INSTRUMENT DAMAGE: Indicates that damage to the instrument or component might occur. This damage might not be covered under the standard warranty. xviii TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 19: Instrument Markings And Symbols

    Table 1 explains the symbols used on Thermo Fisher Scientific instruments. Only a few of them are used on TriPlus 500 HS, which are annotated with an asterisk below. Table 1. Instrument Marking and Symbols (Sheet 1 of 2) Symbol...

  • Page 20: Safety Information And Warnings

    Thermo Fisher Scientific representatives during installation, and repair of TriPlus 500 HS, or parts of it (following the life cycle principle), as well as for the end user of TriPlus 500 HS in the lab during the learning phase, and in routine work.

  • Page 21: Electrical Hazards

    Poor grounding represents a danger for the operator, and might seriously affect the performance of the instrument. Do not connect TriPlus 500 HS to power lines that supply devices of a heavy duty nature, such as motors, refrigerators and other devices that can generate electrical disturbances.

  • Page 22: Other Hazards

    The power supply for TriPlus 500 HS has the symbols I/O on the label for the power switch to indicate On/Off. It is important that the power On/Off switch is accessible to unplug the AC power cord from the power supply/wall outlet in case of emergency.

  • Page 23: Working With Toxic Or Other Harmful Compounds

    Safety Data Sheet (SDS) supplied by the manufacturer, referring to the relevant CAS (Chemical Abstract Service) number. TriPlus 500 HS may require the use of several chemical products with different hazard characteristics, change which are present in vials. Before using these substances, please read the hazard indications and information reported in the SDS supplied by the manufacturer referring to the relevant CAS number.

  • Page 24: Maintenance

    Maintenance Any external cleaning or maintenance must be performed with TriPlus 500 HS turned off and the power cord disconnected. Avoid using solvents and spraying on electrical parts. For the removal of potentially dangerous...

  • Page 25: Disposal

    TriPlus 500 HS for complimentary equipment pick-up and recycling. WARNING The customer has to ensure that TriPlus 500 HS has not been contaminated by any hazardous chemical or biological compounds including (but not limited to) bacteria or viruses.

  • Page 27: Chapter 1 Getting Familiar With Your Triplus 500 Headspace Sampler

    Getting Familiar with Your TriPlus 500 Headspace Sampler This chapter provides information to familiarize users with the TriPlus 500 Headspace Sampler (TriPlus 500 HS or HS sampler). Contents • Introduction • Instrument Basics • Label Location on the Instrument •...

  • Page 28: Introduction

    The TriPlus 500 HS is directly coupled with a TRACE 1300 Series GC and controlled by a Thermo Scientific Chromatography Data System, through the GC touch screen, if available, or the virtual GC user interface.

  • Page 29: Instrument Basics

    (TriPlus 500 HS-12), upgradeable to 120-vial capacity with the addition of a vial loader and a tray holder with three removable 40-vial trays (TriPlus 500 HS-120). The TriPlus 500 HS-120 can be further extended to 240-vial capacity with an additional tray holder and three 40-vial trays on the top of the GC.
  • Page 30 Getting Familiar with Your TriPlus 500 Headspace Sampler Instrument Basics Figure 2. TriPlus 500 HS-120 Coupled with a TRACE 1300 Series GC TriPlus 500 Vial Loader Sample Vial Trays TriPlus 500 HS-120 TRACE 1300 GC TRACE 1310 GC TriPlus 500 HS-120...
  • Page 31 TRACE 1300 Series GC top cover. Each sample tray has its own identification initials according to its position on the TriPlus 500 HS, GC, or on both. This allows for proper management of the vials during the sample sequence. Thermo Scientific...
  • Page 32 Getting Familiar with Your TriPlus 500 Headspace Sampler Instrument Basics ATTENTION DO NOT PLACE a vial into the 12-position rotating carousel when using the Vial Loader and the Vial Trays. The Vial Loader carries one vial at a time into the pre-defined position 1 of the carousel, while the other 11 positions are all potentially used for vial recovering.

  • Page 33: Label Location On The Instrument

    Getting Familiar with Your TriPlus 500 Headspace Sampler Label Location on the Instrument Label Location on the Instrument Figure 4 shows the location of the labels attached to the TriPlus 500 HS. Figure 4. Labels Alert label Instrument model, Serial number, Part...

  • Page 34: Incubation Group

    Getting Familiar with Your TriPlus 500 Headspace Sampler Incubation Group Incubation Group The Incubation Group includes the Sample Vial Carousel and the Incubation Oven. See Figure Figure 5. Incubation Group Sample vial carousel Incubation oven housing Sample Vial Carousel The sample vial carousel consists of a 12-seat rotating carousel, numbered from 1 to 12, for vial housing.

  • Page 35: Incubation Oven

    Getting Familiar with Your TriPlus 500 Headspace Sampler Sampling Path Incubation Oven The incubation oven consists of a heated box including a 12-seat rotating oven carousel. The vials are accurately thermostatted up to 300 °C and a fan provides a constant and uniform temperature.
  • Page 36 Getting Familiar with Your TriPlus 500 Headspace Sampler Sampling Path Figure 6. Sampling Path Sampling system housing Valve-column interface The sampling valve group is protected by a thermally insulated metal box. The valve is heated up to 225 °C with the standard factory-installed valve. An optional high temperature valve is available to heat the valve in range from 150 °C up to 300 °C.

  • Page 37: Pneumatic Connections

    GC through a dedicated adapter. Note For installing and connecting the adapter between the Back SSL injector module on the GC and the TriPlus 500 HS, refer to the TriPlus 500 Headspace Sampler Hardware Manual. • An inlet port marked Aux gas input (550 kPa - 80 PSI Max) for connecting the auxiliary gas supply from the cylinder.

  • Page 38: Electrical Connections

    Getting Familiar with Your TriPlus 500 Headspace Sampler Electrical Connections Electrical Connections The electrical interface includes the power section and the electronic module to make electrical and communication connections among the HS sampler and the external devices. Figure Figure 8.

  • Page 39: Status Panel

    The electronic module includes: • Two ports marked TFS BUS (IN) and TFS BUS (OUT) for the interconnection with the TriPlus 500 HS units such as the Vial Loader. • A RJ45 connector marked LAN for connecting to the network.
  • Page 40 Getting Familiar with Your TriPlus 500 Headspace Sampler Status Panel Figure 10. Power On 2. Not Ready status When the HS sampler is in Not Ready status, the Ready light blinks orange. Figure Figure 11. Not Ready Status 3. Ready status When the sampler parameters have reached the setpoint values, the Ready light becomes a solid green.

  • Page 41: Trace 1310 Gc User Interface

    TRACE 1310 GC User Interface TRACE 1310 GC User Interface The Human-Machine Interface (HMI) of the TRACE 1310 GC recognizes the connected TriPlus 500 HS. The relevant icon is visualized on the touch screen to open the Headspace Sampler Main Menu. See Figure Figure 15.

  • Page 42: Vial Loader

    Getting Familiar with Your TriPlus 500 Headspace Sampler Vial Loader Vial Loader The Vial Loader is a device for upgrading the sampling capacity up to 120 vials adding up to three 40-position sample trays. The sampling capacity can be further increased up to 240 vials adding other three 40-position sample trays placed on the back part of a TRACE 1300 Series GC top cover.

  • Page 43: Vial Loader Electronic Module

    Getting Familiar with Your TriPlus 500 Headspace Sampler Vial Loader Vial Loader Electronic Module The electronic module on the back of the Vial Loader includes the connectors to make electrical and communication connections. See Figure Figure 17. Vial Loader Electronic Module...
  • Page 44 • Two ports marked TFS BUS (IN) and TFS BUS (OUT) are for the interconnection with the units of the TriPlus 500 HS as the Vial Loader. • A 9-pin connector marked GC to synchronize the HS sampler with the GC (Ready In/Out and Start In/Out signals).

  • Page 45: Sample Trays

    500 HS, TRACE 1300 Series GC, or on both the instruments: • HS Sample Tray A, B, and C — Sample trays installed on the top cover of a TriPlus 500 • GC Sample Tray A, B, and C — Sample trays installed on the top cover of a TRACE...
  • Page 46 Getting Familiar with Your TriPlus 500 Headspace Sampler Sample Trays Figure 21. Sample Trays Positioning GC Sample Tray C GC Sample Tray B GC Sample Tray A HS Sample Tray A HS Sample Tray B HS Sample Tray C IMPORTANT Because the sample trays have all the same vial numbering from 1 to 40, when programming a sequence of samples it is mandatory to specify the trays used and the number of vials placed in each sample tray.

  • Page 47: Vials Sequence Syntax

    Getting Familiar with Your TriPlus 500 Headspace Sampler Sample Trays Vials Sequence Syntax This section provides the correct syntax for identifying the position of the vials when compiling the sample table (column Position) for the sequence of the samples. Syntax for Sample Carousel Sample Carousel: •...

  • Page 48: Positioning The Vials In The Sample Tray

    Getting Familiar with Your TriPlus 500 Headspace Sampler Sample Trays Positioning the Vials in the Sample Tray ATTENTION The sample tray can contain up to forty 10 mL or 20/22 mL vials arranged on four rows. Each sample tray MUST contain vials of the same type. DO NOT place 10 mL and 20/22 mL vials into the same sample tray.

  • Page 49: Barcode Reader

    Getting Familiar with Your TriPlus 500 Headspace Sampler Barcode Reader Barcode Reader This device allows the instrument to read barcodes on labels. Stickers with the barcode containing the codified data of the samples are glued on the wall of the relevant sample vials.

  • Page 50: Barcode Reader Description

    Getting Familiar with Your TriPlus 500 Headspace Sampler Barcode Reader Barcode Reader Description The Barcode Reader consists of the following main parts. See Figure Figure 24. Barcode Reader Optical scanner Rotating plate for Rotation 10 mL and 20/22 mL mechanism...
  • Page 51 7 mm Dimension QR-code: 7x7mm Barcode application window 1D o QR-code. • The operator can test reading the barcode label on the vial through the TriPlus 500 Web Interface. • For details see Chapter 7, “Using the TriPlus 500 Web Interface.”...
  • Page 52 Getting Familiar with Your TriPlus 500 Headspace Sampler Barcode Reader Supported Types of Barcode Symbols Table 1 lists a series of barcode types that can be decoded. Table 1. Barcode Symbology (Sheet 1 of 2) Minimum Maximum Barcode Type Description...
  • Page 53 Getting Familiar with Your TriPlus 500 Headspace Sampler Barcode Reader Table 1. Barcode Symbology (Sheet 2 of 2) Minimum Maximum Barcode Type Description Barcode Symbology Length Length 2 of 5 Interleaved Numerical characters. Unlimited ISBT 128 Used for labeling of human blood.

  • Page 54: Heated/Cooled Tray

    Getting Familiar with Your TriPlus 500 Headspace Sampler Heated/Cooled Tray Heated/Cooled Tray The Heated/Cooled Tray is an optional accessory for heating/cooling all the vials on the sample tray through the circulation of a fluid underneath the dedicated heated/cooled tray holder plate. See Figure Figure 26.

  • Page 55: Triplus 500 Web Interface

    Headspace Sampler Hardware Manual: TriPlus 500 Web Interface The TriPlus 500 Web Interface is a web-based application used for instrument control of the TriPlus 500 HS. The operator can check the network configuration and the status of the instrument. Also, it is possible to perform calibration of the tray holder, leak check and a reading test of the barcode label on the vial.

  • Page 57: Chapter 2 Operating Principles

    Operating Principles This chapter describes the operating principles of the TriPlus 500 HS. Contents • Introduction to the Headspace Technique • Pneumatics • Analytical Cycle Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 58: Introduction To The Headspace Technique

    • The sample is not vaporized in the GC, therefore its partial decomposition is avoided and interference of other components eliminated • Taking advantage of its intrinsically high modularity, TriPlus 500 HS expands the capabilities of the TRACE 1300 Series GC...

  • Page 59: Pneumatics

    Operating Principles Pneumatics Pneumatics The pneumatic diagram of TriPlus 500 HS is schematically shown in Figure Figure 29. Pneumatic Diagram • The carrier gas is regulated by the GC. The carrier gas flows into the HS sampler through the adapter gas tubing block installed on the Back SSL injector. The carrier gas used with the GC can be helium, nitrogen, hydrogen, air or argon.

  • Page 60: Analytical Cycle

    MANIFOLD Restrictor Valve Charcoal Trap VALVE OVEN Carrier Flow from SSL 1 mL Split Flow to SSL NEEDLE HEATER Sample LOADING Loop Needle Splitter Column 6-port Sampling Valve To Detector INCUBATION OVEN TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 61 Splitter Column 6-port Sampling Valve To Detector INCUBATION OVEN Note The flow rate used during Stand-by, if active, is the same as set for the Purge level in the method. See Table Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 62: Incubation

    During the incubation phase the vial remains in the incubation oven at a constant temperature. Note During the incubation phase it is possible to stir the sample in the vials choosing the appropriate vial shaking mode Slow, Medium, or Fast. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 63: Pressurization

    (Pressure set-Pressure initial)/Time, where the time is set by the operator, until P2 reaches the pressurization pressure set. Wait the Pressure Equilibration Time set by the operator. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 64: Leak Check

    Valve Charcoal Trap VALVE OVEN Carrier Flow from SSL 1 mL Split Flow to SSL NEEDLE HEATER Sample LOADING Loop Needle Splitter Column 6-port Sampling Valve To Detector Sample Vial INCUBATION OVEN TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 65: Loop Filling

    Restrictor Charcoal Trap VALVE OVEN Carrier Flow from SSL 1 mL Split Flow to SSL NEEDLE HEATER Sample LOADING Loop Needle Splitter Column 6-port Sampling Valve To Detector Sample Vial INCUBATION OVEN Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 66: Injection

    The On/Off valve is open to vent the residual pressure of the vial. – The sampling valve is set to load position when injection time is expired. – The needle is removed from the vial. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 67 The sampling valve is in a position depending on the injection time. – The needle is removed from the vial. • MHE (Multiple Headspace Extraction) – Same as single injection, but venting is always selected. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 68: Purging

    Note The purge level set for the post-injection purging is also applied to the Stand-by purge, when selected. See Table 2. If purging is not necessary, consider not checking this option to reduce gas consumption during Stand-by. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 69: Chapter 3 Setting Up Through The Trace 1310 User Interface

    Setting Up Through the TRACE 1310 User Interface This chapter contains the instructions to configure your TriPlus 500 HS and to edit the parameters through the TRACE 1310 GC user interface (touch screen) or through the TRACE 1310 Virtual Touch Screen software.

  • Page 70: User Interface Overview

    User Interface Overview The HMI (Human-Machine Interface) of the TRACE 1310 GC recognizes the connected TriPlus 500 Headspace sampler. The relevant icon is visualized on the touch screen and must be selected to open the Headspace Sampler Main Menu. See Figure Figure 37.

  • Page 71: Data Entry Keys

    1. Press the name of parameter to enter or modify it, for example Incubator temperature. The value in the setpoint field highlights. 2. Type the desired value, for example 250, and then press Enter to confirm the modification. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 72: Shortcut Key

    These keys indicating when not-currently visible menu items exist. They appear in two ways: • Down arrow key — indicates that you can scroll downward. • Up arrow key — indicates that you can scroll upward. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 73: Configuring The Instrument

    Setting Up Through the TRACE 1310 User Interface Configuring the Instrument Configuring the Instrument Press the Configuration icon on the touch screen Headspace Sampler Main Menu to configure the TriPlus 500 Headspace Sampler. See Figure Figure 39. Touch Screen Main Menu: Configuration TriPlus 500 HS The Configuration menu appears.
  • Page 74 • HS Description — Type a name you want to assign to your instrument. • IP Address — Visualizes the IP address to allow the LAN control of the TriPlus 500 HS through the Thermo Scientific™ Chromeleon™ or TraceFinder™ Chromatography Data System (CDS).

  • Page 75: Editing The Instrument Method

    See Figure Figure 41. Touch Screen Main Menu: Instrument Control The Instrument Control menu appears. See Figure 42 Figure 43 Figure 42. Instrument Control Menu (1) Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 76 Pressure, Time, and Rate. The selected mode activates the associated parameters. • Vial Pressure — Defines the target pressure level at which the vial is pressurized. Set a value in the range 0.00–500.00 kPa. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 77 (equilibration time or GC time). At each sampling an analysis is performed. • Injection time — Defines the time of the sample transfer to the GC. Set a value in the range 0.00–999.00 minutes. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 78 • Needle purge time — Defines the duration of the purging phase. Set a value in the range 0.00–999.00 minutes. • Constant purge — The purge gas flows continuously in the system. Choose On to enable the function, or Off to disable it. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 79: Performing Samples Sequence

    Figure 45. Inject Menu • Tray Holder — Define the tray holder in use. – Select HS when the vials are inserted in the 12-seal rotating carousel of the TriPlus 500 Headspace Sampler. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 80 – Select Loader when the vials are inserted in the 40-position sample trays installed on the top cover of the TriPlus 500 Headspace Sampler. • Sample tray — Enabled only when Tray Holder is selected to Loader. Select among the sample trays A, B, or C.

  • Page 81: Monitoring The Instrument Status

    Monitoring the Instrument Status Monitoring the Instrument Status Press the Status icon on the touch screen Headspace Sampler Main Menu to monitor the status of the TriPlus 500 Headspace Sampler. See the example in Figure Figure 46. Touch Screen Main Menu: Status The Status menu appears.

  • Page 82: Information Page

    Software — Indicates the software and firmware version of the TriPlus 500 HS and the Vial Loader when present. Hardware — Indicates the serial number of the TriPlus 500 HS and the serial number of the Vial Loader when present.

  • Page 83: Chapter 4 Setting Up Through Chromeleon Cds

    Setting Up Through Chromeleon CDS This chapter contains the instructions to configure your TriPlus 500 HS and to edit the parameters through the Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS). Contents • Configuring the TriPlus 500 HS Through Chromeleon CDS •...

  • Page 84: Configuring The Triplus 500 Hs Through Chromeleon Cds

    Run your Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS), then open the TriPlus 500 HS Configuration window. Figure 50 shows the Configuration dialog window of Chromeleon™ CDS for the TriPlus 500 Figure 50. Chromeleon™ Configuration Dialog Window for the TriPlus 500 HS TriPlus 500 Headspace Sampler User Guide...

  • Page 85: Hardware Configuration

    Hardware Configuration In case of off-line editing, in this area you set the hardware configuration of your TriPlus 500 Carrier Control Board — Select the check box in case the instrument is equipped with the optional local carrier gas control.

  • Page 86: Error Handling

    Setting Up Through Chromeleon CDS Configuring the TriPlus 500 HS Through Chromeleon CDS Loop Volume — Type the volume of the sample loop installed on the sampling valve. The range is from 0.25 to 3.00 mL. The default loop volume is 1.0 mL.

  • Page 87: Editing Method Parameters Through Chromeleon Cds

    Figure 51. Chromeleon™ Headspace Setting Page The Headspace Settings page contains the following areas: • “Incubation” page 62 • “Pressurization” page 62 • “Loop Filling” page 62 • “Injection” page 63 • “Venting and Purging” page 63 Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 88: Incubation

    Set a value in the range 0.00–5.00 minutes. Loop Filling Enable loop/sample path temperature control — Select this check box to enable the temperature control of the loop/sample path. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 89: Injection

    Vial enrichment time — Enabled when Injection Mode is set to Enrichment. Specifies the time between one enrichment of and the next. Set a value in the range 0.50–999.99 minutes. Venting and Purging In this area set the venting and purging parameters. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 90: Chromeleon Hs Sampler Control Panel

    • Needle purge time — Defines the duration of the purging phase after sampling. Set a value in the range 0.00–999.99 minutes. Chromeleon HS Sampler Control Panel This section describes how to check on the current Control Panel of the TriPlus 500 HS while working in Chromeleon CDS. Figure 52 shows an example of the Status window for the HS Sampler.
  • Page 91 – Clear Error — Clears a possible error happened. – Force Standby — Forces TriPlus 500 HS in standby condition. – Close — Exits Offline Commands window. • Diagnostics — By selecting this button the following page appears and allows you to find diagnostics information.
  • Page 92 – Firmware — Indicates Serial number, Firmware version, and other information of the HS sampler and of the Vial Loader when present. See the example of Figure Figure 56. Firmware Info Tab TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 93 Chromeleon HS Sampler Control Panel – Errors — Indicates the diagnostic of the possible errors. See the example in Figure Figure 57. Errors Tab Note For details please refer to the Chromeleon documentation. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 94: Vials Sequence

    “Vials Sequence Syntax” page 21. This section shows an example of vials sequences according to the configuration of TriPlus 500 HS: TriPlus 500 HS-12, TriPlus 500 HS-120, and TriPlus 500 HS-120+120. Figure Figure 59, and Figure Figure 58.
  • Page 95 Setting Up Through Chromeleon CDS Vials Sequence Figure 60. TriPlus 500 HS-120+120 Vials Sequence Page Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 96 Setting Up Through Chromeleon CDS Vials Sequence TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 97: Chapter 5 Setting Up Through Tracefinder Cds

    Setting Up Through TraceFinder CDS This chapter contains the instructions to configure your TriPlus 500 HS and to edit the parameters through the Thermo Scientific™ TraceFinder™ Chromatography Data System (CDS). Contents • Configuring TriPlus 500 HS Through TraceFinder CDS •...

  • Page 98: Configuring Triplus 500 Hs Through Tracefinder Cds

    Run your Thermo Scientific™ TraceFinder™ Chromatography Data System (CDS), then open TriPlus 500 HS Configuration window. Figure 61 shows the Configuration dialog window of TraceFinder CDS for TriPlus 500 HS. Figure 61. TraceFinder™ Configuration Dialog Window for TriPlus 500 HS The Configuration dialog window is subdivided into the following areas: •...

  • Page 99: Connection

    Connection status is shown to the right of the button (e.g: disconnected, connected). Hardware Configuration In case of off-line edit, in this area you set the hardware configuration of your TriPlus 500 HS. Headspace Unit — Select this check box to choice your instrument. The following sub-selections are available: HS Carrier/X-Line control board —...

  • Page 100: User Configuration

    Setting Up Through TraceFinder CDS Configuring TriPlus 500 HS Through TraceFinder CDS User Configuration In this area you specify the instrument name and enable/disable the reading of the barcode. Instrument name — Type a name you want to assign to your HS sampler.

  • Page 101: Editing Method Parameters Through Tracefinder Cds

    Figure 62. TraceFinder HS Sampler Instrument Method Setup Page The Method Setup page contains the following areas: • “Incubation” page 76 • “Pressurization” page 76 • “Loop Filling” page 76 • “Injection” page 77 Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 102: Incubation

    Vial Pressurization Rate (kPa/Min) — Visualized when Vial Pressurization Mode is set to Rate. Defines the rate of the pressure change in the range 30.00–3000.00 kPa/min. Loop Filling In this area set the following parameters: TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 103: Injection

    Set a value in the range 0.50–999.99 minutes. Venting and Purging In this area set the venting and purging parameters. • Disable Vial Venting — Select this check box to disable the vial venting performed after sampling. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 104: Advanced

    In this area set the temperature of the optional object when present. Heated/Cooled Tray Temperature (°C) — Check this box to enable the control of the temperature. In the adjacent area type a value in the range 4–70 °C. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 105: Tracefinder Road Map Home Page Status Tabs

    TraceFinder Road Map Home Page Status Tabs TraceFinder Road Map Home Page Status Tabs This section describes how to check on the current status of the TriPlus 500 HS while working in TraceFinder CDS. Status pages are located on TraceFinder Roadmap-Home page. Just highlight TriPlus 500 HS from the Roadmap Status tab scroll list and see the TriPlus 500 HS sampling system tab.
  • Page 106 TraceFinder Road Map Home Page Status Tabs Actuals — Visualizes the status of temperatures, pressures, vials and valve currently in the system. Note For details please refer to the Thermo Xcalibur User Guide -TriPlus 500 Headspace Sampler Setup P/N 31716109. TriPlus 500 Headspace Sampler User Guide...

  • Page 107: Method Development

    Method Development This chapter provides information for developing a method with the TriPlus 500 HS. Contents • Usage Notes • Processing a Sample Vial • Method Developing Workflow • Analytical Troubleshooting General Guidelines Usage Notes This section provides some advice about the headspace technique for optimizing parameters that can affect the sensitivity, precision, and accuracy of an analysis.

  • Page 108: Temperatures

    To optimize your selection of vial pressurization, run a series of vials using different pressures, and interpret the optimum condition from peak areas versus vial pressurization. Note Optimization is valid only for analysis of a specific sample at a specific oven temperature. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 109: Shaking Conditioning

    K. Sample Vials TriPlus 500 HS can use 10 mL and 20/22 mL vials. The vial size may change with each method in use in a sequence. The vial can have crimp or screw caps with flat or rounded bottoms and can have clear or amber glass.

  • Page 110: Vial Filling

    • The vial requires the use of a metallic cap. • If the cap is loose the vial may leak. • Adjust the crimper and close the vial again. • The cap should be flat. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 111: Missing And Wrong Size Vials

    HS sampler performs the action selected in Error Handling. IMPORTANT While the TriPlus 500 HS recognizes the type of the vial by its height, the instrument does not actually distinguish between 20 mL and 22 mL vials. There are commercial 22 mL vials that are not compatible with the TriPlus 500 HS.

  • Page 112: Principles Of Multiple Headspace Extraction (Mhe)

    In a normal single extraction, the following steps are employed in order to sample an aliquot of the headspace gas: • The vial is thermostatted in order for equilibrium to be established between the phases in the headspace vial. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 113 A(ref ) — Area we would measure if all of the analyte were in the headspace (corresponding to the measured signal from a similar gas standard). A(0) — Measured area of the analyte peak from the first extraction. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 114 In this case, you also arrive at the correct value by determining the total amount of analyte by comparing the measured sum of analyte areas to a calibration measurement using data from a single determination of a gas standard. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 115 A(tot) = 2 * A(ref ) In this case A(tot) will be over-determined by a factor of two relative to A(ref ). In other words, the content of analyte will be over-determined by a factor of two. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 116 MHE technique. Title: ‘Static Headspace-Gas Chromatography – Theory and Practice’ Authors: Bruno Kolb and Leslie S. Ettre, Second Edition. Published by: John Wiley & Sons, Inc. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 117: Processing A Sample Vial

    Processing a Sample Vial Processing a Sample Vial Figure 65 Figure 66 show how the TriPlus 500 HS processes a sample vial according to the configuration HS-12 and HS-120. Figure 65. TriPlus 500 HS-12 - Vial Processed Workflow User Actions...

  • Page 118: User Actions

    5. Repeat the previous steps for all the required sample vials to analyze. Instrument Actions Note At this point it is assumed that GC and TriPlus 500 HS are properly installed, all the required gas supplies are open, the method parameters for the GC (oven, injector and detector) and the sampler have been properly set, and the sequence of samples has been defined.
  • Page 119 3. At the end of the Incubation phase the sampler lifts the sample vial in the injection position. The sample needle penetrates into the sample vial and the system starts the Pressurization phase. See Figure Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 120 The duration of the Leak check is 12 seconds. – If a leak is detected, the instrument behaves according to the Error handling configured (for example Ignore and Inject, Fake Injection, or Abort). TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 121 AUX Gas Vent Flow On/Off Restrictor Valve Charcoal Trap Carrier Flow from SSL 1 mL Split Flow to SSL Sample SAMPLING Loop Needle Splitter Column 6-port Sampling Valve To Detector Sample Vial Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 122 Valve Charcoal Trap Carrier Flow from SSL 1 mL Split Flow to SSL Sample LOADING Loop Needle Splitter Column 6-port Sampling Valve To Detector 9. The Purging phase ends the analytical sequence. TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 123: Method Developing Workflow

    Note It is assumed that TRACE 1300 Series GC and TriPlus 500 HS are properly installed. The Chromeleon or TraceFinder Chromatography Data System (CDS) is installed on your PC. For details refer to Chapter 2 and Chapter 3 of the TriPlus 500 Headspace Sampler Hardware Manual.
  • Page 124 Open the gas supplies for GC and TriPlus 500 HS-12. b. Power on the GC by placing the power switch in the On (up) position marked I. c. Power on the TriPlus 500 HS-12 by placing the power switch in the On (up) position marked I.
  • Page 125 (or screws according to the vial type). d. Place the vial into the 12-seat rotating carousel or into a 40-position sample tray according to version of your TriPlus 500 HS-12 or HS-120. e. Repeat the previous steps for all the required sample vials to analyze.
  • Page 126 Open the Sample Table of the CDS in use. b. Compile the Sample Table using the correct syntax for identifying the position of the vials into the 12-seat rotating carousel or into the sample trays. See Figure TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 127 Method Developing Workflow Figure 76. Vial Sequence Syntax Syntax for Sample Carousel (without Vial Loader) Position: 1....12 Syntax for Sample Trays on TriPlus 500 HS Position on Sample Tray A: HS:A1....HS:A40 Position on Sample Tray B HS:B1....HS:B40 Position on Sample Tray C: HS:C1....HS:C40...

  • Page 128: Analytical Troubleshooting General Guidelines

    Method parameters Check recommended Method parameters Ghost peaks Sample carryover from previous Purge adequately the sampling valve and analysis the loop Increase loop/sample path temperature Vial contaminated Use pre-cleaned vials and proper septa TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 129 Any parameter such as the injector, oven or detector temperature can contribute to bad peak shape Check the column type and connections Unexplained chromatographic Dirty purge gas Check the purge gas supply for impurities peaks Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 130 Method Development Analytical Troubleshooting General Guidelines TriPlus 500 Headspace Sampler User Guide Thermo Scientific...

  • Page 131: Chapter 7 Using The Triplus 500 Web Interface

    Using the TriPlus 500 Web Interface This chapter provides the instructions for using the TriPlus 500 Web Interface. Contents • Introduction • Menu Bar • Logging Out of the TriPlus 500 Web Interface Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

  • Page 132: Introduction

    To access the TriPlus 500 Web Interface 1. Take note of the Actual IP address of the TriPlus 500 HS to control. 2. Open your Internet browser and type the actual IP address of the TriPlus 500 HS to check: http://actual IP address for example: http://169.254.250.4...

  • Page 133: Menu Bar

    Menu Bar Menu Bar The menu bar of TriPlus 500 Web Interface includes the following six menus. Each menu lists a series of functions available. All the functions are always visualized but the access is allowed only to a limited number, according to the level access.

  • Page 134: Login Menu

    Using the TriPlus 500 Web Interface Menu Bar Login Menu In the menu bar select Login and perform the User Login. The operator must type the following User name and Password: – User name = user – Password = ThermoFisher Press Login.

  • Page 135: Status Menu

    Using the TriPlus 500 Web Interface Menu Bar • Network Configuration — Check and /or reconfigures the network parameters of the TriPlus 500 HS. In case of re-configuration of the network parameters, save and apply the changes selecting Save&Apply, then Reboot HS and Reboot Loader when the Vial Loader is present.
  • Page 136 • Alarms — Lists the HS sampler and Vial Loader active alarms that happened in the system. The operator can clear the list selecting Clear Alarms. • HS Live Logs — Shows the operations the TriPlus 500 HS is performing. To clear the list select Clear log.
  • Page 137 Using the TriPlus 500 Web Interface Menu Bar • Loader Logs — Shows the operations the Vial Loader is performing. To clear the list select Clear log. • Loader Selftest — This function is unauthorized for the operator. Thermo Scientific...

  • Page 138: Installation/Tools Menu

    Using the TriPlus 500 Web Interface Menu Bar Installation/Tools Menu This menu includes the following functions: • Tray Holder Calibration —The purpose of this operation is to calibrate the motion of the Vial Loader on the holder plate without the sample trays installed.
  • Page 139 When ready select Start. The Vial Loader performs the automated calibration routine at the end of which the Vial Loader goes back in home position and the routine is stopped. Note See also the section Calibrating Tray Holder in the TriPlus 500 Headspace Sampler Hardware Manual.
  • Page 140 Using the TriPlus 500 Web Interface Menu Bar Before starting the leak check, the system asks users to load an empty vial into the position 1 of the HS carousel. The operator can use the default parameters visualized the first time this function is selected, or modify them.
  • Page 141 Using the TriPlus 500 Web Interface Menu Bar The operator can perform the test in sequential or manual mode. • Sequential mode — Performs automatically the reading of the barcode labels on the vials placed into a sample tray. –...

  • Page 142: Service Menu

    Manufacturing Menu Shows the menu page but the functions listed are unauthorized for the operator. Logging Out of the TriPlus 500 Web Interface To log out from the TriPlus 500 Web Interface, select Hello from the bar menu then select Logout.

  • Page 143: Chapter 8 Ordering Parts

    Ordering Parts To order parts for the TriPlus 500 HS instrument, refer to the TriPlus 500 Headspace Spare Parts Guide. Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 144 Ordering Parts TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 145 FSE Field Service Engineer CIP Carriage and Insurance Paid To ft foot cm centimeter CPU central processing unit (of a computer) g gram <Ctrl> control key of the keyboard GC gas chromatography- gas chromatograph Thermo Scientific TriPlus 500 Headspace Sampler User Guide...
  • Page 146 Ω ohm K Kelvin kg kilogram p pico (10 kPa kilopascal Pa pascal PCB printed circuit board l length PN part number L liter psi pounds per square inch LAN Local Area Network TriPlus 500 Headspace Sampler User Guide Thermo Scientific...
  • Page 147 A brief voltage surge of up to several thousand volts, with a duration of less than 50 μs. V volt Vac volts, alternating current Vdc volts, direct current VGA Video Graphics Array VL Vial Loader Thermo Scientific TriPlus 500 Headspace Sampler User Guide...

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