AUTOMATED ELECTROPHORESIS
Experion
™
Quick Guide
Turn On Experion Electrophoresis Station and Launch Experion Software
1
Equilibrate Kit Reagents
2
Remove the DNA 12K stain, gel (or gel-stain solution, if available), and loading buffer from storage,
I
and equilibrate to room temperature
Invert each tube several times; vortex the contents and briefly centrifuge
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Note: Protect stain and gel-stain solution from light at all times.
Prepare the Gel-Stain Solution
3
Transfer 200 µl DNA 12K gel
I
Add 10 µl DNA stain
(blue
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Transfer gel-stain solution to a spin filter tube and centrifuge at 1,500 x g for 10 min
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Note: Filtered gel-stain stored at 4°C may be used for up to 1 month. Discard after 1 month; do not refilter.
Prepare the DNA Samples and DNA 12K Ladder
4
Remove the DNA 12K ladder (clear cap) from storage, briefly centrifuge, and then vortex to mix;
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place the tube of ladder on ice
Prepare dilutions of DNA sample in TE buffer or DNase-free water to concentrations within the
I
linear range of this assay (0.5–50 ng/µl total DNA); make sure the salt concentration of the sample
does not exceed 250 mM KCl (or 250 mM NaCl), 15 mM MgCl
Prime the Chip
5
Remove a DNA chip from its packaging and place it on the chip
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platform in the Experion priming station
Add 9 µl gel-stain solution into the gel priming well
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Close the lid of the priming station, set the pressure to C,
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and set the time to 1
Press the Start button
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When priming is complete, remove the primed chip from the
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priming station
Flip the chip over, and visually inspect the microchannels for
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trapped air bubbles or incomplete priming
Note: Do not touch the glass surface of the chip.
DNA 12K Analysis Kit
(green
cap) to a DNase-free 0.65 ml microcentrifuge tube
cap) to the tube of 200 µl DNA 12K gel; vortex and briefly centrifuge
2
DNA
Gel
priming
well
1K = C3
12K = C1
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