BIO RAD Experion User Quick Manual
BIO RAD Experion User Quick Manual

BIO RAD Experion User Quick Manual

Automated electrophoresis dna 12k analysis kit

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AUTOMATED ELECTROPHORESIS
Experion
Quick Guide
Turn On Experion Electrophoresis Station and Launch Experion Software
1
Equilibrate Kit Reagents
2
Remove the DNA 12K stain, gel (or gel-stain solution, if available), and loading buffer from storage,
I
and equilibrate to room temperature
Invert each tube several times; vortex the contents and briefly centrifuge
I
Note: Protect stain and gel-stain solution from light at all times.

Prepare the Gel-Stain Solution

3
Transfer 200 µl DNA 12K gel
I
Add 10 µl DNA stain
(blue
I
Transfer gel-stain solution to a spin filter tube and centrifuge at 1,500 x g for 10 min
I
Note: Filtered gel-stain stored at 4°C may be used for up to 1 month. Discard after 1 month; do not refilter.

Prepare the DNA Samples and DNA 12K Ladder

4
Remove the DNA 12K ladder (clear cap) from storage, briefly centrifuge, and then vortex to mix;
I
place the tube of ladder on ice
Prepare dilutions of DNA sample in TE buffer or DNase-free water to concentrations within the
I
linear range of this assay (0.5–50 ng/µl total DNA); make sure the salt concentration of the sample
does not exceed 250 mM KCl (or 250 mM NaCl), 15 mM MgCl

Prime the Chip

5
Remove a DNA chip from its packaging and place it on the chip
I
platform in the Experion priming station
Add 9 µl gel-stain solution into the gel priming well
I
Close the lid of the priming station, set the pressure to C,
I
and set the time to 1
Press the Start button
I
When priming is complete, remove the primed chip from the
I
priming station
Flip the chip over, and visually inspect the microchannels for
I
trapped air bubbles or incomplete priming
Note: Do not touch the glass surface of the chip.
DNA 12K Analysis Kit
(green
cap) to a DNase-free 0.65 ml microcentrifuge tube
cap) to the tube of 200 µl DNA 12K gel; vortex and briefly centrifuge
2
DNA
Gel
priming
well
1K = C3
12K = C1

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Summary of Contents for BIO RAD Experion

  • Page 1 DNA 12K Analysis Kit ™ Quick Guide Turn On Experion Electrophoresis Station and Launch Experion Software Equilibrate Kit Reagents Remove the DNA 12K stain, gel (or gel-stain solution, if available), and loading buffer from storage, and equilibrate to room temperature Invert each tube several times;...
  • Page 2 700-7163 Experion DNA Chips, 10 700-7165 Experion DNA 12K Reagents and Supplies, for 10 chips, includes 650 µl DNA 12K gel, 40 µl DNA stain, 20 µl DNA 12K ladder, 750 µl DNA 12K loading buffer, 3 spin filters 700-7251...

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