Thermo Scientific Dionex IonPac AS32-Fast-4mm Analytical Product Manual page 6

Contents
5. Troubleshooting ................................................................................. 25
5.1 High Back Pressure ...................................................................................................................................... 27
5.1.1 Finding the Source of High System Pressure............................................................................................... 27
5.1.2 Replacing Column Bed Support Assemblies (4 mm and 2 mm columns only) ........................................ 27
5.1.3 Filter Eluent.................................................................................................................................................... 28
5.1.4 Filter Samples ................................................................................................................................................ 28
5.2 High Background .......................................................................................................................................... 28
5.2.1 Preparation of Eluents ................................................................................................................................... 28
5.2.2 A Contaminated Trap Column ...................................................................................................................... 29
5.2.3 Contaminated Dionex CR-TC Column ........................................................................................................ 29
5.2.4 A Contaminated Guard or Analytical Column ............................................................................................ 29
5.2.5 Contaminated Hardware................................................................................................................................ 29
5.2.6 A Contaminated Suppressor.......................................................................................................................... 29
5.3 Inconsistent Retention Times ..................................................................................................................... 29
5.3.1 Drifting to Shorter Retention Time............................................................................................................... 30
5.3.2 Drifting to Longer Retention Time............................................................................................................... 30
5.3.3 Oscillating Retention Times.......................................................................................................................... 30
5.4 Poor Peak Resolution ................................................................................................................................... 31
5.4.1 Loss of Column Efficiency ........................................................................................................................... 31
5.4.2 Analyte Specific Efficiency Loss ................................................................................................................. 32
5.4.3 Shortened Retention Times ........................................................................................................................... 33
5.4.4 Loss of Front-End Resolution ....................................................................................................................... 34
5.5 Spurious Peaks ............................................................................................................................................... 34
Appendix A - Column Care ................................................................ 35
6.
6.1 Recommended Operati on Pressures......................................................................................................... 35
6.2 Column Start-Up ........................................................................................................................................... 35
6.3 Column Storage ............................................................................................................................................. 35
6.4 Chemical Purity Requirements.................................................................................................................. 35
6.4.1 Inorganic Chemicals ...................................................................................................................................... 36
6.4.2 Deionized Water ............................................................................................................................................ 36
6.4.3 Solvents .......................................................................................................................................................... 36
6.5 Column Cleanup............................................................................................................................................ 37
6.5.1 Choosing the Appropriate Cleanup Solution ............................................................................................... 38
6.5.2 Column Cleanup Procedure .......................................................................................................................... 39
Appendix B - Quality Assurance Reports ............................................ 40
7.
Appendix C - Additional Information ................................................. 42
8.
General Information on PEEK™ Tubing ............................................................................................... 42
8.1
Thermo Scientific
065763-01
Product Manual for Dionex IonPac AS32-Fast-4µm Columns
For Research Use Only. Not for use in diagnostic procedures.
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