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Turner Designs Opti-Trace User Manual

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Fluorescence Solutions for Water Monitoring
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Version 1.2
December 14, 2018
P/N 998-6700
TURNER DESIGNS
st
1995 N. 1
Street
San Jose, CA 95112
Phone: (877) 316-8049
(408) 749-0994
FAX:
(408) 749-0998
Turner Designs
Opti-Trace
Fluorometer
User's Manual

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  • Page 1 Fluorescence Solutions for Water Monitoring ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Turner Designs Opti-Trace Fluorometer User’s Manual Version 1.2 December 14, 2018 P/N 998-6700 TURNER DESIGNS 1995 N. 1 Street San Jose, CA 95112 Phone: (877) 316-8049 (408) 749-0994 FAX: (408) 749-0998...

  • Page 2: Table Of Contents

    Table of Contents TABLE OF CONTENTS ........................2 INTRODUCTION ..........................3 ............................3 ESCRIPTION ........................3 NSPECTION AND ETUP INSTALLATION ..........................4 ....................4 NSTALLATION NSTALLATION ...................... 4 OWER AND TILITIES EQUIRED ................4 OCATION OF RACE AND AMPLING OINT ......................

  • Page 3: Introduction

    By combining the Opti-Trace with a data collection system, the exact level of a tracer dye can be measured, which in turn can be used to control the addition of treatment chemicals assuming the tracer dye is tagged to the chemical of interest.

  • Page 4: Installation

    (Note: Higher flow rates may require more frequent cleaning of the basket strainer and sample cell). Drain: Sample output from the Opti-Trace should be piped to a drain with no back pressure, i.e. drain pipes must be below the unit.

  • Page 5: Mechanical Connections

    The Opti-Trace is rated for light industrial environments. Do not install within 10 feet/3 meters of devices such as large generators that generate a strong electromagnetic field. It is extremely important to eliminate air entrapment in the sample line. The best way to accomplish this is to sample from the center of the pipe or from the side of the pipe.

  • Page 6: Section 1 - Calibrate Your Data Collection System To The Opti-Trace

    2. Section 2 will describe how to adjust your Opti-Trace if you want to use different settings from the factory standard. The adjustment will consist of setting upper (20 mA) and lower (4mA) output currents that correspond to known concentration levels. These two levels will be chosen to enable a controller to vary the strength of a chemical.
  • Page 7 Measure the blanking current 1. Connect the Opti-Trace fluorometer to the Power Supply and Data Collection System as shown in Fig 2 below. 2. Using a syringe, inject a sample of the de-ionized water through the Luer-Lock valve. Set your data collection system to read “0’ for this sample.

  • Page 8: Section 2 - Adjusting The Output Of The Opti-Trace

    Section 2 – Adjusting the Output of the Opti-Trace The Opti-trace is shipped from the factory calibrated to a DI water "Blank" and a known concentration of "fluorescent dye" specific to the optical configuration ordered. (See Test Data Results Report included with Sensor documentation).

  • Page 9: Section 3 - Characterizing For Standalone Use

    6. Remove the DVM temporarily installed for calibration purposes. Return the Opti-Trace to service by turning on the process water flow. 7. Note that if your sample is out of the range of the Opti-Trace, it may not be possible to adjust the output to 12 mA.
  • Page 10 Procedure Measure the blanking current 1. Connect the Opti-Trace fluorometer to the Power Supply and Digital Multimeter as shown below. 2. Using a syringe, inject a sample of the de-ionized water through the Luer-Lock. Measure the output current of the sensor (equal to the blank current). Note the reading in Table 1 as indicated.
  • Page 11 Solution Measured Sensor Output Current Blank Solution Blank Current Sample Solution Opti-Trace User’s Manual Page...
  • Page 12 “x” = sensor output value in mA, “m” is the slope of the response = (sample concentration – blank concentration)/(sample output current – blank output current), “c” is a constant equal to the value of “y” when “x” = 0 Opti-Trace User’s Manual Page...

  • Page 13: Recommended Measurement Practices

    Measure a sample of water with dye using the Opti-Trace b) Clean the Opti-Trace flowcell using the brush included in the accessories c) Re-measure the sample used in step (a). d) Calculate the difference in readings and determine whether the difference is outside acceptable limits as defined in your application.

  • Page 14: Removing Unwanted Bubbles In The Syringe

    5. Linear Range and Quenching The linear range is the concentration range in which the Opti-Trace output is directly proportional to the concentration of the fluorophore. The linear range begins with the smallest detectable concentration, and spans...

  • Page 15: Temperature Considerations

    (See following table 2 for examples of dye temperature fluorescent dyes compensation coefficients). Information on how to apply these corrections is included in the Turner Designs Application Note: A Practical Guide to Flow Measurements, see the following URL: http://docs.turnerdesigns.com/t2/doc/appnotes/998-5000.pdf Correction (/...

  • Page 16: Terms

    Warranty Terms Turner Designs warrants the Opti-Trace and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions: ...

  • Page 17: Warranty Service

    Repair service will be billed on a fixed price basis, plus any applicable duties and/or taxes. Shipment to Turner Designs should be prepaid. Your bill will include return shipment freight charges.

  • Page 18: Opti-Trace Specifications

    5 – 30 VDC Input Voltage 4 – 20 mA Signal Output Light Source Light Emitting Diode Detector Photodiode Warm up time 5 seconds Length: 5.75” Dimensions, (length excludes Width: 3.5” connector) Depth: 3.0” Weight 5.0 ozs, 160 gm Opti-Trace User’s Manual Page...

  • Page 19: Opti-Trace Wiring Table

    Appendix B Opti-Trace Wiring Table Opti-Trace Function Connection Wire PSU – Positive Connection Supply Voltage 5 – 30 VDC PSU – Ground Connection Black Supply Ground, 0VDC Multimeter “A” Connection Orange Signal Out to data logger, “A”, 4 – 20 mA DC Multimeter “Common”...

  • Page 20: Appendix C - Pre-Installation/Installation Checklist

    (no back pressure, max. 10 ft/3 m rise). Customer Requirements - Electrician 1. ________ ________ Provide 5-30 VDC, 1.5 Watts electrical service to the Opti-Trace. 2. ________ ________ Terminate flow switch wiring to your alarm system. Continued on next page. Opti-Trace User’s Manual Page...

  • Page 21: Final Requirements - Customer

    Customer’s Responsibilities: This checklist outlines the work that is required prior to start-up. Work is necessary to ensure quality and proper operation. However, if any of these requirements cannot be met, contact Turner Designs Technical Services. In some cases, alternative procedures will still provide reliable results.
  • Page 22 Appendix D Figure 7 – Inlet Plumbing Kit Details TO OPTI-TRACE UNIT OPTI-TRACE INLET PLUMBING – PVC PLUMBING Opti-Trace User’s Manual Page...
  • Page 23 Figure 8 – Drain Plumbing Kit Details Sample Outlet From Opti-Trace Unit Opti-Trace User’s Manual Page...
  • Page 24 Figure 9 - Opti-Trace Fluorometer, Showing Identification of Key Parts Opti-Trace User’s Manual Page...
  • Page 25 Turner Designs 1991 N 1 Street. San Jose, CA 95112 Phone: (408) 749-0994 Toll Free (877) 316-8049 © Copyright 2004, Turner Designs P/No. 998-6700 Opti-Trace User’s Manual Page...

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