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1. See
Section 2, "Operation".
requirements.
2. See
"Quality Assurance
Reports". Run the Production Test Chromatogram as
a system check.
3. See
"Column Care"
for column cleanup and long-term storage
recommendations.
©DIONEX Corporation, 1997, 1998, 2002
PRODUCT MANUAL
AG2 GUARD COLUMN
®
(4 x 50 mm, P/N SP4834)
®
AG3 GUARD COLUMN
(4 x 50 mm, P/N 043182)
AG4 GUARD COLUMN
®
(4 x 50 mm, P/N 035310)
AG5 GUARD COLUMN
®
(4 x 50 mm, P/N 035396)
CG2 GUARD COLUMN
®
(4 x 50 mm, P/N 035370)
AS2 ANALYTICAL COLUMN
®
(4 x 250 mm, P/N SP4831)
AS3 ANALYTICAL COLUMN
®
(4 x 250 mm, P/N 043181)
AS4 ANALYTICAL COLUMN
®
(4 x 250 mm, P/N 035311)
AS5 ANALYTICAL COLUMN
®
(4 x 250 mm, P/N 035395)
CS2 ANALYTICAL COLUMN
®
(4 x 250 mm, P/N 035371)
Note operation precautions and chemical purity
Document No. 032226
Revision 14
12 November 2002
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Related Manuals for Thermo Scientific IONPAC AG2 GUARD COLUMN
Summary of Contents for Thermo Scientific IONPAC AG2 GUARD COLUMN
- Page 1 PRODUCT MANUAL IONPAC AG2 GUARD COLUMN ® (4 x 50 mm, P/N SP4834) IONPAC ® AG3 GUARD COLUMN (4 x 50 mm, P/N 043182) IONPAC AG4 GUARD COLUMN ® (4 x 50 mm, P/N 035310) IONPAC AG5 GUARD COLUMN ® (4 x 50 mm, P/N 035396) IONPAC CG2 GUARD COLUMN...
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Page 2: Table Of Contents
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 2 of 14 TABLE OF CONTENTS SECTION 1 - INTRODUCTION ..................... 3 Overview ................................... 3 Chemicals ................................. 3 Column Storage ................................ 3 Recommended Operating Pressures ........................3 Applications ................................3 SECTION 2 - OPERATION ......................4 General Operation Precautions .......................... -
Page 3: Section 1 - Introduction
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 3 of 14 SECTION 1 - INTRODUCTION Overview This manual describes how to prepare this column for operation. The initial start-up procedure usually consists of installing a column in the Chromatography Module, flushing it with deionized water to remove the storage solution, and equilibrating it with eluent. Chemicals The chemicals used to make eluents, standards, regenerants and post-column reagents must be of very high purity in order to obtain reliable, consistent and accurate analytical results. -
Page 4: Section 2 - Operation
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 4 of 14 SECTION 2 - OPERATION General Operation Precautions CAUTION < 5% Organic Solvent in the Eluent Filter and Degas Eluents Filter Samples Chemicals Required Obtaining reliable, consistent and accurate results requires eluents that are free of ionic and spectrophotometric impurities. Chemicals, solvents and deionized water used to prepare eluents must be of the highest purity available. -
Page 5: Section 3 - Standard Conditions
IonPac AS2, AS3, AS4, AS5, CS2 Manual Document No. 032226-14 Page 5 of 14 SECTION 3 - STANDARD CONDITIONS Standard Reagents Table 1 Storage Solutions, Eluents and Regenerants Column Storage Standard Standard Solution Eluent Regenerant ANION ANALYTICAL AND GUARD COLUMNS IonPac ®... -
Page 6: Standard Flow Rates And Back Pressures
IonPac AS2, AS3, AS4, AS5, CS2 Manual Document No. 032226-14 Page 6 of 14 Standard Flow Rates and Back Pressures Table 2 Standard Flow Rates and Back Pressures Column Standard Expected Maximum Flow Rate Back Pressure Back Pressure Anion Analytical and Guard Columns IonPac AS2/AG2 3.0 mL/min 600 psi/200 psi... -
Page 7: Section 4 - Initial Start-Up Procedures
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 7 of 14 SECTION 4 - INITIAL START-UP PROCEDURES For the initial start-up of both anion and cation analytical columns, prepare the eluent listed on the test chromatogram, set the pump to the proper flow rate and perform the following initial start-up procedures. -
Page 8: Section 5 - Troubleshooting Guide
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 8 of 14 SECTION 5 - TROUBLESHOOTING GUIDE The purpose of the Troubleshooting Guide is to help you solve operating problems that may arise while using DIONEX columns. For more information on problems that originate with the Ion Chromatograph (IC) or the suppressor, refer to the Troubleshooting Guide in the appropriate operator’s manual. -
Page 9: Replacing Column Bed Support Assemblies
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 9 of 14 5.1.2 Replacing Column Bed Support Assemblies If the column inlet bed support is determined to be the cause of the high back pressure, it should be replaced. To change the inlet bed support assembly, refer to the following instructions, using one of the two spare inlet bed support assemblies included in the Ship Kit. -
Page 10: Bed Support Descriptions
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 10 of 14 5.1.3 Bed Support Descriptions The following bed support assemblies are supplied with the different DIONEX Column Lines: O-RING BED SUPPORT FLOW Column Line Assembly Component Description Descriptions ® 4-mm HPIC (Tefzel 035843 Bed Support... - Page 11 IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 11 of 14 BED SUPPORT IonPac P/N 35311 S/N 9835 CHROM AT OGRAPHY FLOW Column Line Assembly Component Description Descriptions 4-mm IonPac (1/4-28 Liquid Line Fitting) 042310 Bed Support Assembly 041375 Bed Support 039835 Seal Washer...
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Page 12: High Background Or Noise
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 12 of 14 High Background or Noise In a properly working system, the background conductivity level for the standard eluent system is shown in Table 1, “Storage Solutions, Eluents and Regenerants.” If the background conductivity is higher than listed in Table 1, “Storage Solutions, Eluents and Regenerants,”... -
Page 13: A Contaminated Self-Regenerating Suppressor (Srs ) Or The Micromembrane Suppressor (Mms)
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 13 of 14 5.2.5 A Contaminated Self-Regenerating Suppressor (SRS ) or the MicroMembrane Suppressor (MMS) ™ ™ If the above items have been checked and the problem persists, the SRS or the MMS is probably causing the problem. A. -
Page 14: Loss Of Front End Resolution
IonPac AS3, AS4, AS5, CS2 Document No. 032226-14 Page 14 of 14 one of the proportioned eluents is less than 5%. C. Column contamination can lead to a loss of column capacity. This is because all of the ion exchange sites will no longer be available for the sample ions. - Page 15 IonPac ® Analytical (4 x 150 mm) Product No. SP4831 Serial No. : 0019 Pressure (PSI) : 290 Date : 11/19/99 9:46:28 AM 0019 Eluent: 3 mM Na /2 mM NaOH 10.0 Flow Rate: 3.0 mL/min Detection: Suppressed Conductivity at 30 µSFS ®...
- Page 16 IonPac ® Analytical (4 x 250 mm) Product No. 43181 Serial No. : 3649 Pressure (PSI) : 480 Date : 5/18/00 10:04:50 AM 3649 Eluent: 2.8 mM NaHCO / 2.2 mM Na Flow Rate: 3.0 mL/min 14.0 Detection: Suppressed Conductivity at 30 µSFS 12.0 ASRS ®...
- Page 17 IonPac ® Analytical (4 x 250 mm) Product No. 35311 Serial No. : 16 Pressure (PSI) : 710 Date : 9/7/00 8:15:02 AM Eluent: 2.8 mM NaHCO / 2.2 mM Na Flow Rate: 2.0 mL/min 16.0 Detection: Suppressed Conductivity at 30 µSFS 14.0 ASRS ®...
- Page 18 IonPac ® Analytical (4 x 250 mm) Product No. 35395 Serial No. : 8874 Pressure (PSI) : 871 Date : 1/9/01 5:27:47 PM 8874 Eluent: 2.2 mM Na / 2.8 mM NaHCO Flow Rate: 2.0 mL/min 14.0 Detection: Suppressed Conductivity 12.0 ASRS ®...
- Page 19 IonPac ® Analytical (4 x 250 mm) Product No. 35371 Serial No. : 4604 Pressure (PSI) : 570 Date : 4/27/00 11:32:21 AM 4604 Eluent: 10 mM Oxalic acid/7.5 mM Citric 4.50x10 acid, pH 4.35 w/LiOH 4.00x10 Eluent Flow Rate: 1.0 mL/min 3.50x10 Post-Column Reagent: 3x10...
- Page 20 IonPac AS3, AS4, AS5, CS2 Column Care Document No. 03226C14 Page 1 of 4 COLUMN CARE RECOMMENDED OPERATION PRESSURES Operating a column above its recommended pressure limit can cause irreversible loss of column performance. The maximum recommended operating pressure for the columns covered by this manual are listed in Table 2, "Standard Flow Rates and Back Pressures."...
- Page 21 IonPac AS3, AS4, AS5, CS2 Column Care Document No. 03226C14 Page 2 of 4 BASE-SOLUBLE CONTAMINANTS This procedure is used to elute anionic contaminants from anion exchange columns. Typical eluents are formulated from carbonate/bicarbonate mixtures, borate and hydroxide. A. Prepare a 500 mL solution of 10X eluent concentrate. See Table 1, "Storage Solutions, Eluents and Regenerants." B.
- Page 22 IonPac AS3, AS4, AS5, CS2 Column Care Document No. 03226C14 Page 3 of 4 ACID-SOLUBLE CONTAMINANTS This procedure is used to elute cationic contaminants from cation exchange columns. Typical eluents are formulated from hydrochloric acid. A. Prepare a 500 mL solution of 10X eluent concentrate. See Table 1, "Storage Solutions, Eluents and Regenerants." B.
- Page 23 IonPac AS3, AS4, AS5, CS2 Column Care Document No. 03226C14 Page 4 of 4 ORGANIC CONTAMINANTS CAUTION Eluents used on columns containing low cross-linked resins must contain less than 5% organic solvents. A. Prepare a 500 mL solution of 5% acetonitrile in deionized water having a specific resistance of 18.2 megohm-cm. B.