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IonPac AS3, AS4, AS5, CS2 Column Care
Document No. 03226C14
Page 1 of 4
COLUMN CARE
RECOMMENDED OPERATION PRESSURES
Operating a column above its recommended pressure limit can cause irreversible loss of column performance. The maximum
recommended operating pressure for the columns covered by this manual are listed in Table 2, "Standard Flow Rates and Back
Pressures."
COLUMN STORAGE
For short-term storage, the strongest eluent in use can be used as the storage solution. For long-term storage, use the storage
solution listed in Table 1, "Storage Solutions, Eluents and Regenerants." Flush the column for 10 minutes with the storage solution.
Cap both ends securely, using the plugs supplied with the column.
COLUMN CLEANUP
The following column cleanup protocols have been divided into three general isocratic protocols to remove acid-soluble, base-
soluble or organic contaminants. They can be combined into one gradient protocol if desired but the following precautions should
be observed.
Always ensure that the cleanup protocol used does not switch between eluents which may create high pressure eluent interface
bands in the column. High pressure bands can disrupt the uniformity of the packing of the column bed and irreversibly damage
the performance of the column. High pressure bands in the column can be created by pumping successive eluents through the
column that are not miscible or that have eluent components that will precipitate out in the other eluent. The precipitation of the
salts in solvents during column rinses can result in very high pressure bands. High viscosity mixing bands can be created between
two eluents having solvents with a very high energy of mixing.
CAUTION
Eluents used on columns containing low cross-linked resins must contain less than 5% organic solvents.
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