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IonPac AS3, AS4, AS5, CS2 Column Care
ORGANIC CONTAMINANTS
Eluents used on columns containing low cross-linked resins must contain less than 5% organic solvents.
A. Prepare a 500 mL solution of 5% acetonitrile in deionized water having a specific resistance of 18.2 megohm-cm.
B. Disconnect the suppressor from the analytical column. If your system is configured with both a guard column and
an analytical column, reverse the order of the guard and analytical column in the eluent flow path. Double check that
the eluent flows in the direction designated on each of the column labels. Direct the effluent from the outlet line of
the guard column to a separate waste container.
When cleaning an analytical column and a guard column in series, ensure that the guard column is placed after the
analytical column in the eluent flow path. Contaminants that have accumulated on the guard column can be eluted onto
the analytical column and irreversibly damage it. If in doubt, clean each column separately.
C. Set the pump flow rate to that listed in Table 2, "Standard Flow Rates and Back Pressures."
D. If your eluent contains a solvent that is not compatible with 5% acetonitrile, rinse the column for 15 minutes with
deionized water before pumping the 5% acetonitrile over the column.
E. Pump a 5% acetonitrile solution through the column for 30-60 minutes.
F. If your eluent contains a solvent that is not compatible with 5% acetonitrile, rinse the column for 15 minutes with
deionized water before pumping eluent over the column.
G. Reconnect the suppressor column to the analytical column and place the guard column in line between the
injection valve and the analytical column if your system was originally configured with a guard column.
H. Equilibrate the column(s) with eluent before resuming normal operation.
Document No. 03226C14
CAUTION
CAUTION
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