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IonPac AS3, AS4, AS5, CS2 Column Care
ACID-SOLUBLE CONTAMINANTS
This procedure is used to elute cationic contaminants from cation exchange columns. Typical eluents are formulated from
hydrochloric acid.
A. Prepare a 500 mL solution of 10X eluent concentrate. See Table 1, "Storage Solutions, Eluents and Regenerants."
B. Disconnect the suppressor from the analytical column. If your system is configured with both a guard column and
an analytical column, reverse the order of the guard and analytical column in the eluent flow path. Double check that
the eluent flows in the direction designated on each of the column labels. Direct the effluent from the outlet line of
the guard column to a separate waste container.
When cleaning an analytical column and a guard column in series, ensure that the guard column is placed after the
analytical column in the eluent flow path. Contaminants that have accumulated on the guard column can be eluted onto
the analytical column and irreversibly damage it. If in doubt, clean each column separately.
C. Set the pump flow rate to that listed in Table 2, "Standard Flow Rates and Back Pressures."
D. If your eluent contains a solvent that is not compatible with the 10X eluent concentrate, rinse the column for 15 minutes
with deionized water before pumping the 10X eluent concentrate over the column.
E. Pump 10X eluent concentrate solution through the column for 30-60 minutes.
F. Reconnect the suppressor to the analytical column and place the guard column in line between the injection
valve and the analytical column if your system was originally configured with a guard column.
G. Equilibrate the column(s) with eluent before resuming normal operation.
Document No. 03226C14
CAUTION
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