Table Of Contents - Thermo Scientific IONPAC AG2 GUARD COLUMN Product Manual

IonPac AS3, AS4, AS5, CS2
SECTION 1 - INTRODUCTION ..................................................................................................... 3
1.1 Overview ................................................................................................................................................................... 3
1.2 Chemicals ................................................................................................................................................................. 3
1.3 Column Storage ........................................................................................................................................................ 3
1.4 Recommended Operating Pressures ....................................................................................................................... 3
1.5 Applications .............................................................................................................................................................. 3
SECTION 2 - OPERATION ............................................................................................................. 4
2.1 General Operation Precautions .............................................................................................................................. 4
2.2 Chemicals Required ................................................................................................................................................. 4
2.2.1 Inorganic Chemicals .................................................................................................................................................. 4
2.2.2 Solvents .................................................................................................................................................................... 4
2.2.3 Deionized Water ........................................................................................................................................................ 4
SECTION 3 - STANDARD CONDITIONS .................................................................................... 5
3.1 Standard Reagents ................................................................................................................................................... 5
3.2 Standard Flow Rates and Back Pressures .............................................................................................................. 6
SECTION 4 - INITIAL START-UP PROCEDURES ...................................................................... 7
SECTION 5 - TROUBLESHOOTING GUIDE ............................................................................... 8
5.1 High Back Pressure ................................................................................................................................................ 8
5.1.1 Finding the Source of High System Pressure ............................................................................................................ 8
5.1.2 Replacing Column Bed Support Assemblies ............................................................................................................. 9
5.1.3 Bed Support Descriptions ....................................................................................................................................... 10
5.2 High Background or Noise .................................................................................................................................... 12
5.2.1 Preparation of Eluents ............................................................................................................................................. 12
5.2.2 A Contaminated Anion Trap Column ...................................................................................................................... 12
5.2.3 A Contaminated Guard or Analytical Column ......................................................................................................... 12
5.2.4 Contaminated Hardware .......................................................................................................................................... 12
5.2.5 A Contaminated Self-Regenerating Suppressor (SRS™) or the MicroMembrane™ Suppressor (MMS) ............... 13
5.3 Poor Peak Resolution ............................................................................................................................................ 13
5.3.1 Loss of Column Efficiency ...................................................................................................................................... 13
5.3.2 Poor Resolution Due to Shortened Retention Times .............................................................................................. 13
5.3.3 Loss of Front End Resolution ................................................................................................................................. 14
5.4 Spurious Peaks ...................................................................................................................................................... 14
Document No. 032226-14

TABLE OF CONTENTS

Page 2 of 14