Thermo Scientific SimpliAmp Installation And Operational Manual page 5

Prepare samples using MicroAmp
attached caps
1. Separate the blue tray and retainer by squeezing the release
catch as indicated in the graphic.
Release catch
1
™
MicroAmp 96-Well Retainer
2
™
MicroAmp 96-Well Tray
3
2. Place the blue tray on the 96-well base.
3. Load the tube strips on the tray.
4. Pipette the reaction mixture into the tubes.
5. Seal the tube strip using a MicroAmp
tubes strips with cap strips" on page 5 for instructions.
6. Remove the blue tray containing the sealed tube strips from
the 96-well base and place the tray and sealed tube strips into
the instrument.
™
MicroAmp 8-Tube Strip with Attached Caps (0.2-mL)
1
MicroAmp ™ 96-Well Tray
2
MicroAmp ™ Splash Free 96-Well Base
3
SimpliAmp Thermal Cycler Installation and Operation Quick Reference
™
tube strips with
™
1
2
1
3
™
cap strip. See "Seal
Seal tubes strips with cap strips
IMPORTANT!
Apply significant downward pressure on the
sealing tool in all steps to form a complete seal on top of the tubes.
1. Align and place the cap strips on the tubes.
2. Seal the cap strips using the rocking capping tool:
a. Slip your fingers through the handle with the holes in
the tool facing down.
b. Place the holes in the tool over the first eight caps in a
row.
c. Rock the tool back and forth a few times to seal the
caps.
d. Repeat for remaining caps in the row, then for all
remaining rows.
Prepare samples using MicroAmp
1. Set the black tray on a 96-well base.
2. Place the reaction tubes in the black tray.
™
MicroAmp Reaction Tube with Cap (0.2-mL)
1
™
MicroAmp 96-Well Tray for VeriFlex
2
™
MicroAmp Splash Free 96-Well Base
3
3. Pipette the reaction mixture into the reaction tubes.
4. Cap the tubes.
5. Remove the black tray with sealed reaction tubes from the
96-well base and place the tray and sealed tubes into the
instrument.
Reaction Tubes
™
1
2
3
™
Blocks
5