Leica CM3050 S Instruction Manual page 50

Problem
Anti-roll plate damaged after
adjustment.
Thick/thin sections.
Tissue sticks to the anti-roll
plate.
Flattened sections curl up as
soon as anti-roll plate is lifted
from the knife.
50
6.
Causes
- Anti-roll plate protrudes too far
beyond the knife edge. Adjust-
ment was done in the direction
of the knife.
- Wrong temperature for the
tissue to be sectioned.
- Knife profile inappropriate for
the specimen to be sectioned.
- Ice build-up on the knife back.
- Handwheel rotation not uni-
form or inappropriate rotation
speed.
- Knife not clamped tightly
enough.
- Specimen disc not clamped
correctly.
- Embedding medium poured
onto cold specimen disc; spec-
imen comes off the disc once
frozen.
- Blunt knife.
- Incorrect clearance angle.
- Specimen dried out.
- Anti-roll plate too warm or
incorrectly adjusted.
- Static electricity.
- Fat on corner or edge of anti-
roll plate.
- Knife rusty.
- Static electricity or draft.
- Anti-roll plate too warm.
Troubleshooting, applications tips
Remedies
- Replace anti-roll plate; in
future, lift away from the knife
when aligning.
- Handle anti-roll plate more
carefully.
- Select correct temperature.
- Wait until correct temperature
has been reached.
- Use knife with different profile
(c or d) or possible switch to
disposable blade system.
- Remove ice.
- Adapt speed.
- Check knife clamping.
- Check disc clamping.
- Place embedding medium on
luke-warm specimen disc,
inside the cryochamber place
the specimen onto the disc and
freeze in quick-freeze shelf.
- Use different part of knife or
replace knife.
- Set correct clearance angle.
- Prepare new specimen.
- Cool or readjust anti-roll plate.
- Eliminate cause.
- Remove fat (alcohol).
- Remove rust.
- Eliminate cause.
- Cool anti-roll plate.
Instruction Manual V1.1 02/2000