Leica CM3050 S Instruction Manual page 49

6. Troubleshooting, applications tips
Problem
Sections curl on anti-roll plate.
Scraping noise during section-
ing stroke and specimen return
stroke.
Ondulated sections.
Chatter marks form during
sectioning.
Condensation forms on anti-roll
plate and knife during cleaning.
Leica CM3050 S – Cryostat
Causes
- Anti-roll plate does not pro-
trude far enough beyond the
knife edge.
- Anti-roll plate protrudes too far
beyond the knife edge and is
scraping against the specimen.
- Knife damaged.
- Edge of anti-roll plate damaged.
- Specimen insufficiently frozen
onto specimen disc.
- Specimen disc not clamped
tightly enough.
- Specimen holder ball joint not
clamped.
- Knife not clamped tightly
enough.
- Selected section thickness too
thick - specimen has come off
the specimen disc.
- Specimen very hard and inho-
mogeneous.
- Blunt knife.
- Knife profile inappropriate for
the type of specimen to be
sectioned.
- Wrong clearance angle.
- Brush, forceps, cloth or other
cleaning item too warm.
Remedies
- Readjust correctly.
- Readjust correctly.
- Use different part of the knife
or replace knife.
- Replace anti-roll plate.
- Refreeze specimen onto disc.
- Check disc clamping.
- Check ball joint clamping.
- Check knife clamping.
- Refreeze specimen onto disc.
- Increase section thickness; if
possible, reduce specimen
surface.
- Use different part of knife or
replace knife.
- Use knife with a different pro-
file.
- Readjust clearance angle set-
ting.
- Accessories and cleaning
material to be used pre-cooled
only. - Store on storage shelves
inside the cryochamber.
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