Freezing Specimens Onto Specimen; Optional Accessories For Freezing - Leica CM3050 S Instruction Manual

5. Operation

5.5.2 Freezing specimens onto specimen

discs with 90 ° prism
(Instruments w/specimen cooling only)
In instruments with specimen cooling, the 90 °
prism, which is inserted into the specimen head,
is an additional option to freeze specimens at
maximum temperature .
4
Installing the 90 ° prism
• Loosen screw (3) at specimen head.
• Insert pin (1) of prism completely into recep-
tacle (2) in the specimen head.
Important: the entire rear surface of the
prism must fit tightly against the specimen
head surface!
• Retighten screw (3) at specimen head.
The prism now forms a horizontal surface for
quick specimen freezing.
Freezing specimens with the 90 ° prism
• Apply sufficient amount of embedding medi-
um onto ambient temperature specimen disc,
add specimen and orient.
• Insert specimen disc with specimen into the
receptacle (4) of the prism.
• Press to activate menu item 'Fast Object
Freezing' and activate function by pressing
(see chapter 5.3.4 'Menu functions' item 4).
Leica CM 3050 S – Cryostat
The specimen head is now automatically cooled
down to the lowest temperature (-50 °C).
• Once the specimen is frozen, remove the
disc from the prism and store it in the quick-
freeze shelf until inserting it into the speci-
men head.
• Press again to activate menu item 'Fast
Object Freezing' and deactivate the function
by pressing
5.3.4 'Menu functions' item 4).
The specimen head temperature now automati-
cally returns to the previously selected set val-
ue.
• Loosen screw (3) at specimen head and re-
move prism (1).
• Store prism inside the cryochamber.

5.5.3 Optional accessories for freezing

specimens onto specimen discs
and for removing frozen specimens
Mobile heat extractor
In addition to the stationary heat extractor (part
of standard delivery), a mobile heat extractor is
available as an optional accessory - see Ap-
pendix 'Ordering information - accessories ):
• Store heat extractor in chamber to precool.
• Place onto specimen in quick-freeze shelf.
• Remove as soon as specimen is frozen.
once again (see chapter
41