Table Of Contents - Leica SP5 confocal User Manual

Table o f C ontents
System S tartup . ............................................................................................................................... 3
System S hutdown . .......................................................................................................................... 4
Mounting a s ample . ....................................................................................................................... 5
Finding t he s ample b y e ye ........................................................................................................... 5
Configuration M enu . ..................................................................................................................... 6
Turning t he L asers o n ................................................................................................................... 6
Setting I mage B it D epth . .............................................................................................................. 6
Selecting a b eam p ath s etting . ................................................................................................... 6
Scanning a l ive i mage .................................................................................................................... 8
Creating a n ew b eam p ath s etting . ........................................................................................... 8
Simultaneous v s S equential s canning m odes . ...................................................................... 8
Setting u p S equential S canning ( Recommended) . .............................................................. 9
Image O ptimisation P rocedure . .............................................................................................. 1 0
Capturing a M ulti-­-Wavelength 2 D I mage ( Optical S lice) . ............................................... 1 1
Saving I mages . ............................................................................................................................... 1 1
Capturing a M ulti-­-Wavelength 3 D I mage ( Z S tack) . ......................................................... 1 2
Acquiring I mages a t d ifferent s tage p ositions ( Mark a nd F ind) . ................................. 1 4
Acquiring a T ime-­-Lapse . ............................................................................................................ 1 4
Appendix . ........................................................................................................................................ 1 5
Exporting I mages . ........................................................................................................................ 1 5
FRAP . ................................................................................................................................................ 1 6
FRET A cceptor P hotobleaching . .............................................................................................. 2 5
FRET S ensitised E mission . ........................................................................................................ 3 2
Spectral I maging ........................................................................................................................... 4 2
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