Configuration M Enu; Turning T He L Asers O N; Setting I Mage B It D Epth; Selecting A B Eam P Ath S Etting - Leica SP5 confocal User Manual

zero. Pressing the lower Z set button drops the lens to its lowest position. This
is useful when you want to change to a different cover glass on the same slide if
you're using an oil immersion lens. Dropping the lens is always good practice
when changing slides too.
3. To view brightfield select an empty filter cube position using the buttons on the
front of the microscope.
4. Switch on the transmitted light using the TL/IL button on the left side of the
microscope. Adjust the intensity up/down with the INT buttons.
5. At this point you can either change to a higher magnification lens if required or
begin the scanning process
6. Lenses 60x and above always require a drop of oil between the lens and the
coverglass. 40x lenses can be either a dry or oil type. The software menu used
to select the lenses tells you if the lens requires oil.
Configuration M enu
Turning t he L asers o n
1. Select Configuration tab>Laser
2. Tick the boxes next to the lasers you want to use
3. If using the Argon laser (458/488/514 lines) set the percentage output to
50% in the text box next to the power slide bar then press enter
Setting I mage B it D epth
1. Select Configuration tab>Settings
2. From the Image Resolution drop down list select 12bit
3. The detectors have a 12bit analogue to digital converter so if you select
12bit image resolution this ensures the full dynamic range of the
detector can be utilised
Selecting a b eam p ath s etting
1. Under the Beam Path Settings heading select the drop down list
and scroll down to the heading "User Settings", here you will find
the personal beam path settings you have already configured.
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