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Leica ES2
Leica EZ4
Leica EZ4 W
User Manual

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Summary of Contents for Leica ES2

  • Page 1 Leica ES2 Leica EZ4 Leica EZ4 W User Manual...
  • Page 2 Congratulations! You have just purchased a Leica E Series stereo- In developing our stereomicroscopes, we have microscope – an excellent choice! Viewing placed great emphasis on simple, self-explan- entire objects under magnification imparts atory operation. However, please take the time...
  • Page 3: Table Of Contents

    Chapter Overview Safety and Use Leica ES2 Leica EZ4 and Leica EZ4 W Eyepieces (only for Leica EZ4) Photography Using the Leica EZ4 W Get Set! The Camera Remote Control (Optional) Care, Transport, Contact Persons Specifications Dimensions Leica E Series User Manual...
  • Page 4 Eyepieces (Only for Leica EZ4) Regulatory Compliance Information Changing Eyepieces (Only for Leica EZ4, Open Version) Instructions for Use Eyepieces and Eyecups (Only for Leica EZ4, Open Version) Leica ES2 Dioptric Correction (Only for Leica EZ4, Open Version) Overview: Leica ES2...
  • Page 5 SETUP CAMERA (Camera Settings) SETUP ETHERNET SETUP WiFi Pairing Cameras With Remotes Care, Transport, Contact Persons Care, Transport, Contact Persons Specifications Specifications Dimensions Leica ES2 Leica EZ4 W Leica EZ4, 10× Eyepieces Leica EZ4 without Eyepieces Leica E Series User Manual...
  • Page 6: Safety And Use

    Safety and Use Leica E Series User Manual Safety and Use...
  • Page 7: Symbols Used

    Functional disturbances or damaged instruments Warning of hazardous electrical voltage   This symbol indicates especially impor- tant information that, if not observed, can cause the following: Hazards to personnel Functional disturbances or damaged instruments Leica E Series User Manual Safety and Use...
  • Page 8: Safety Instructions

    The "Safety Concept" brochure contains addi- instrument. Technical Safety tional safety information regarding service To connect the Leica E Series microscopes to work, requirements and the handling of the power, use a power cable that meets the power microscope, accessories as well as general cable specifications in your country.
  • Page 9 Radio interference suppression in compli- directive. ance with EN 55011 class B Noise immunity in compliance with DIN EN 61326-1. Intended for indoor use only in all EU member states, EFTA states and Switzerland. Leica E Series User Manual Safety and Use...
  • Page 10: Regulatory Compliance Information

    Reorient or relocate the receiving antenna. This transmitter must not be co-located or oper- Increase the separation between the equip- ating in conjunction with any other antenna or ment and receiver. transmitter. Leica E Series User Manual Safety and Use...
  • Page 11 (1) l'appareil ne doit pas produire de brouillage, (2) l'utilisateur de l'appareil doit accepter tout brouillage radioélectrique subi, même si le brouillage est susceptible d'en compromettre le fonctionnement. Leica E Series User Manual Safety and Use...
  • Page 12: Instructions For Use

    The power cable is provided Use in clean rooms as the power disconnect device. Leica E Series microscopes can be used in clean rooms without any problems. Leica E Series User Manual Safety and Use...
  • Page 13 – as this manual, repair and service work may mended). increases the risk class. Failure to observe this be carried out by authorized Leica technicians notice poses a risk of eye damage. only.   Accessories...
  • Page 14: Leica Es2

    Leica ES2 Leica E Series User Manual Leica ES2...
  • Page 15: Overview: Leica Es2

    10× fixed eyepieces for eyeglass wearers 2. Carrying handle 3. Two-level magnification changer 10× and 30× 4. Focusing drive 5. Integrated LED incident light illumination 6. Integrated LED transmitted illumination 7. Switch for incident and transmitted light Leica E Series User Manual Leica ES2...
  • Page 16: Transport, Placement And Storage

    Built-in carrying device Setting up the microscope Packing the device away   All Leica E Series microscopes are 1. Place the microscope on a flat tabletop. 1. After use, wind up the cable as shown equipped with a carrying handle for in the illustration.
  • Page 17: The Correct Interpupillary Distance

      You may need a short time to become familiar with using the instrument. Not to worry – after a little while, it will become automatic. Leica E Series User Manual Leica ES2...
  • Page 18: Using The Led Illuminator

    Use transmitted light for transmitted light desired. specimens or transparent objects. Use the combination of both LED illumina- tors for partially transparent objects.   After switching off, the LEDs remain lit for about another 4 seconds. Leica E Series User Manual Leica ES2...
  • Page 19: Focusing

    After you have adjusted the image or right-handed. sharpness at high magnification, it is maintained even if you switch to a lower magnification (parfocal). 2. Set the working distance to approx. 100 mm for coarse focusing. Focusing drive torque Leica E Series User Manual Leica ES2...
  • Page 20: Magnification Display

    Magnification Display   You can read the configured magnifica- tion on the right-hand rotary knob of the Leica ES2. The additional magnification provided by the eyepieces is already incorpo- rated in the scale. Leica E Series User Manual Leica ES2...
  • Page 21: Adjusting The Resistance Of The Focus Drive

    No problem – the resistance can be adjusted individually depending on your personal preferences: 1. Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing. Leica E Series User Manual Leica ES2...
  • Page 22: Leica Ez4 And Leica Ez4 W

    Leica EZ4 and Leica EZ4 W Leica E Series User Manual Leica EZ4 and Leica EZ4 W...
  • Page 23: Overview: Leica Ez4

    10× fixed eyepieces for eyeglass wearers 2. Carrying handle 3. Magnification changer 4. Focusing drive 5. Integrated LED incident light illumination 6. Illumination control 7. Integrated LED transmitted illumination Leica E Series User Manual Leica EZ4 and Leica EZ4 W...
  • Page 24: Instrument Variant: Leica Ez4 W

    4. Carrying handle 5. Magnification changer 6. IR receiver for optional remote control 7. Focusing drive 8. Integrated LED incident light illumination 9. Illumination control 10. Integrated LED transmitted illumination Leica E Series User Manual Leica EZ4 and Leica EZ4 W...
  • Page 25: Transport, Placement And Storage

    Built-in carrying device Setting up the microscope Packing the device away   All Leica E Series microscopes are 1. Place the microscope on a flat tabletop. 1. After use, wind up the cable as shown equipped with a holder for convenient in the illustration.
  • Page 26: The Correct Interpupillary Distance

      You may need a short time to become familiar with using the instrument. Not to worry – after a little while, it will become automatic. Leica E Series User Manual Leica EZ4 and Leica EZ4 W...
  • Page 27: Switching On The Led Illuminator

    Use incident light for plastic, opaque baseplate. objects Use transmitted light for transmitted light specimens or transparent objects. Use the combination of both LED illumina- tors for partially transparent objects. Leica E Series User Manual Leica EZ4 and Leica EZ4 W...
  • Page 28: Controlling The Automatic Shutoff

    The LEDs flash twice slowly and then remain The LEDs flash three times slowly and then switched on. The automatic shutoff is now remain switched on. The automatic shutoff is disabled. now re-enabled. Leica E Series User Manual Leica EZ4 and Leica EZ4 W...
  • Page 29: Illumination Types

    Switch on transmitted light brightness for incident less uniform lighting for emphasizes structures and for transparent objects light objects objects with highly uneven increases the contrast and transmitted light surfaces specimens Leica E Series User Manual Leica EZ4 and Leica EZ4 W...
  • Page 30: Focusing

    (parfocal). 2. Set the working distance to approx. 100 mm for coarse focusing. Focusing drive torque Leica E Series User Manual Leica EZ4 and Leica EZ4 W...
  • Page 31: Magnification Display

    1, 1.25, 1.6, 2, 2.5, 3, 3.5.   Different total magnifications are possi- ble depending on which eyepieces are used with the Leica EZ4. The magnification of the eyepieces is indicated on their outer surfaces, e.g. 10×/20, 16×/16 or 20×/12. Leica E Series User Manual...
  • Page 32: Adjusting The Resistance Of The Focus Drive

    1. Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focus- ing. Leica E Series User Manual Leica EZ4 and Leica EZ4 W...
  • Page 33: Eyepieces (Only For Leica Ez4)

    Eyepieces (Only for Leica EZ4) Leica E Series User Manual Eyepieces (Only for Leica EZ4)
  • Page 34: Changing Eyepieces (Only For Leica Ez4, Open Version)

    Changing Eyepieces (Only for Leica EZ4, Open Version) Changing eyepieces Various eyepieces are available for the Leica EZ4, allowing the stereomicroscope to be adapted even better to the specific require- ments. The following eyepieces are available for selec- tion: Eyepieces replaceable, fixed or adjustable: 10×/20, 16×/16 or 20×/12...
  • Page 35: Eyepieces And Eyecups (Only For Leica Ez4, Open Version)

    Eyepieces and Eyecups (Only for Leica EZ4, Open Version) Eyecups The eyecups can be replaced. Risk of infection   Direct contact with eyepieces is a poten- tial transmission method for bacterial and viral infections of the eye. The risk can be minimized by the use of individual eyecups.
  • Page 36: Dioptric Correction (Only For Leica Ez4, Open Version)

    Dioptric Correction (Only for Leica EZ4, Open Version) Dioptric correction Users with normal vision do not need adjust- Adjusting   The adjustable eyepieces for the Leica able eyepieces. An adjustable eyepiece is suffi- 1. Set the dioptric correction for both EZ4 are also available with built-in diop- cient for mild shortsightedness.
  • Page 37 9. Select the highest magnification and refocus if necessary. Now, if you adjust the magnification from the highest to the lowest level, the specimen is always brought into sharp focus. If not, repeat the process. Leica E Series User Manual Eyepieces (Only for Leica EZ4)
  • Page 38: Graticules (Only For Leica Ez4, Open Version)

    Graticules (Only for Leica EZ4, Open Version) Graticules Available eyepiece inserts   The transparent graticules can be Graticules 10 mm/ 0.1 mm inserted in the adjustable eyepieces of 5 mm/ 0.1 mm the Leica EZ4. They enable easy measurements 5 mm/ 0.05 mm at the specimen.
  • Page 39: Graticules: Preparation

    Always hold the graticule by the edges! 1. Gently press the two small tabs on the snap 1. Screw the insert out counterclockwise. mount together and pull out the insert. Leica E Series User Manual Eyepieces (Only for Leica EZ4)
  • Page 40: Inserting The Graticules

    3. Carefully take hold of the edge of the grati- cule and push it sideways into the holder in the insert. Make sure that both plastic holders snap into place. 5. Insert the eyepiece into the tube. Leica E Series User Manual Eyepieces (Only for Leica EZ4)
  • Page 41: Photography Using The Leica Ez4 W

    Photography Using the Leica EZ4 W Leica E Series User Manual Photography Using the Leica EZ4 W...
  • Page 42: Introduction

    Introduction Thank you for purchasing the Leica EZ4 W by Leica Microsystems. The special design of the Leica EZ4 W makes it a universal, highly versa- tile tool for viewing microscopic specimens and capturing still images or video. Leica E Series User Manual...
  • Page 43: The Basics

    The Basics   Your Leica HD camera has many possibili- Modes of use ties for various applications and condi- tions of use. Below are some basic concepts to 1. SD mode help you get the most performance for your capture onto SD card application.
  • Page 44: Leica Ez4 W: Overview

    Leica EZ4 W: Overview 1. LED status indicator Blinking red means starting up Green means ready 2. Capture button for SD card capture 3. Mode selection panel 4. SD card slot Leica E Series User Manual Photography Using the Leica EZ4 W...
  • Page 45 2. Ethernet socket for connecting an ethernet cable (cable not provided) 3. USB for connecting the provided USB cable Front Infrared window: Receives the signal from the optional remote control. Leica E Series User Manual Photography Using the Leica EZ4 W...
  • Page 46: Unpacking

    The contents include CD with imaging software, If you ordered the handheld remote, you received HDMI cable, and USB cable. the remote with batteries. Leica E Series User Manual Photography Using the Leica EZ4 W...
  • Page 47: Get Set

    Get Set! Leica E Series User Manual Get Set!
  • Page 48: Usb Mode - Capturing And Adjusting Images Using A Computer

    USB port, and attach the other the status light is green and one of the Leica EZ4 W’s field of view, however, is rectan- end of the cable to a USB 2.0 port of your mode button's is also green. This may take gular with a diagonal of 16.4 mm to ensure an...
  • Page 49 4:3 using the controls of the projector or monitor. 6. You are ready to launch the Leica software. Follow the software instructions to adjust and capture an image.
  • Page 50: Sd Mode - Capturing Images Without A Computer Or Without A Wireless Device

    This may take approximately 30 seconds. 4. Insert the SD memory card into the slot on the side of the Leica EZ4 W until the card clicks into place. Leica E Series User Manual Get Set!
  • Page 51 SD Mode – Capturing Images Without a Computer or Without a Wireless Device (Continued) The capture button LED will turn green. The Leica 5. To capture an image onto the SD The image is now saved on the SD card. Images...
  • Page 52: Adjusting Settings While In Sd Mode

    Press and hold the capture button for 5 seconds. Wait for the beep. A new white balance is set. Leica E Series User Manual Get Set!
  • Page 53: Wifi Mode - Capturing And Adjusting Images Using A Wireless Device

    WiFi Mode – Capturing and Adjusting Images Using a Wireless Device 1. Switch on the power to the Leica EZ4 W 3. If a mode button other than WiFi mode is microscope. lit green, then press the WiFi button and wait until both the status LED and the WiFi 2.
  • Page 54: Ethernet Mode - Capturing And Adjusting Images Using A Network

    PC for viewing, capturing, and adjusting the camera image. 2. Switch on the power to the Leica EZ4 W 4. If a mode button other than ethernet mode microscope. is lit green, then press the ethernet button and wait until both the status LED and the ethernet LED mode button is green.
  • Page 55: The Camera Remote Control (Optional)

    HD display. The only function which works in any mode is the "Info" function which shows details about the camera. Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 56: Optional Remote Control

    7. Brightness adjustment up or down 8. Pairing button 9. Show/hide information box 10. White balance set button 11. Call up the camera menu 12. Arrow keys for navigation 13. OK to confirm Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 57: Viewing Images And Movies With The Optional Remote Control

    Viewing Images and Movies With the Optional Remote Control   If you have purchased the optional remote, the Leica HD camera shows images and movie clips directly on an HD moni- tor. 1. Thumbnail view (first press) up/down/left/right  select thumbnail...
  • Page 58: Calling Up The Camera Menu

    3. Press the    and  buttons to select a menu item. 4. Press the button to confirm a menu item. 5. Press the button again to hide the camera menus. Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 59: Color (Automatic White Balance)

    The values for "RED" and "BLUE" cannot be adjusted if the "WB MODE" setting is set to "AUTO".   The "COLOR" function makes it possible to adapt the camera chip to the ambient light so that color-neutral images can be acquired. Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 60: Color (Manual White Balance)

    The "COLOR" function makes it possible to adapt the camera chip 6. Press the button to exit the menu. to the ambient light so that color-neutral images can be acquired. Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 61: Exposure

    3. Set the value for "EXP MODE" to "MANUAL". RESOLUTION GAIN SETUP CAMERA BRIGHTNESS 4. Correct the values for "EXPOSURE", "GAIN" and "GAMMA" until you SETUP ETHERNET GAMMA obtain the desired results for the image. SETUP WIFI Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 62: Resolution

    SETUP WIFI   The "RESOLUTION" menu enables you to define the resolution for the live image, still image capture and video capture individually. This provides optimum results in every situation. Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 63: Setup Camera (Camera Settings)

    This command sets the date and time as well as the format for display- capture. ing them. The following formats are available for selection: YYMMDD – DDMMYY – MMDDYY Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 64: Setup Ethernet Setup Wifi

    Main menu Sub menu Ethernet COLOR IP ADDRESS STATIC EXPOSURE IP ADDRESS (STATIC) 192.168.1.200 RESOLUTION IP SUBNET MASK 255.255.255.0 SETUP CAMERA GATEWAY IP ADDRESS 192.168.1.1 SETUP ETHERNET SETUP WIFI Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 65 Your network provides the access point and the camera connects to your network by WiFi. NAME (SSID) This is the camera name which will appear. You can change the name using the onscreen keyboard. Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 66 If "OK" is selected, you will see a list of WiFi access points nearby. COLOR INTERFACE NET WORK EXPOSURE NAME (SSID) ICC50 W_2345 RESOLUTION SECURITY OPTIONS NONE SETUP CAMERA PASSWORD (8 CHR) SETUP ETHERNET SCAN SSID SETUP WIFI Leica E Series User Manual The Camera Remote Control (Optional)
  • Page 67: Pairing Cameras With Remotes

    Pairing Cameras With Remotes Pairing 3. The microscope only responds to the   The Leica EZ4 W and the remote control remote control commands from this can be paired and then only respond remote control. to each other. This can be helpful when using Resetting to factory setting multiple cameras and remote controls.
  • Page 68: Care, Transport, Contact Persons

    Care, Transport, Contact Persons Leica E Series User Manual Care, Transport, Contact Persons...
  • Page 69: Care, Transport, Contact Persons

    Leica representative. place. Protect the microscope from oil and grease. can find information on worldwide Leica Never grease or oil mechanical parts or representatives Leica website: If any optical surface becomes badly coated sliding surfaces.
  • Page 70: Specifications

    Specifications Due to the technology of the camera, the speci- fications are changing regularly. Please visit website www.leica-microsystems.com/ education for the latest brochure downloads to find the up to date specifications. Leica E Series User Manual Specifications...
  • Page 71: Specifications

    32 GB Integrated power 100 V – 240 V ~ 50 / 60 Hz supply Automatic adjustment to local voltage Power consumption max. 30 W (EZ4 W), 15 W (ES2, EZ4) Leica E Series User Manual Specifications...
  • Page 72: Dimensions

    Dimensions Leica E Series User Manual Dimensions...
  • Page 73: Leica Es2

    Leica ES2 Leica E Series User Manual Dimensions...
  • Page 74 Leica ES2 (Continued) Leica E Series User Manual Dimensions...
  • Page 75: Leica Ez4 W

    Leica EZ4 W Leica E Series User Manual Dimensions...
  • Page 76 Leica EZ4 W (Continued) Leica E Series User Manual Dimensions...
  • Page 77: Leica Ez4, 10× Eyepieces

    Leica EZ4, 10× Eyepieces Leica E Series User Manual Dimensions...
  • Page 78 Leica EZ4, 10× Eyepieces (Continued) Leica E Series User Manual Dimensions...
  • Page 79: Leica Ez4 Without Eyepieces

    Leica EZ4 without Eyepieces Leica E Series User Manual Dimensions...
  • Page 80 Leica EZ4, without Eyepieces (Continued) Leica E Series User Manual Dimensions...
  • Page 81 The Leica Microsystems Industry Division’s focus is to support customers’ pursuit Denmark ∙ Ballerup 4454 0101 4454 0111 of the highest quality end result. Leica Microsystems provide the best and most France ∙ Nanterre Cedex 811 000 664 1 56 05 23 23 innovative imaging systems to see, measure, and analyze the microstructures in Germany ∙...

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